Determination of binding parameters of 2-(Ferrocenylmethylamino) Benzonitrile and 3-(Ferrocenylmethylamino)Benzonitrile WITH 1,1-Diphenyl-2-Picrylhydrazyl free radical

Abstract

Spectrophotometric technics based assay were developed for the determination of binding parameters like binding constant and binding free energy of two potential antioxidants ferrocene derivatives: 2-(ferrocenylmethylamino)benzonitrile (2FMAB), 3-(ferrocenylmethylamino)benzonitrile (3FMAB), and a standard ascorbic acid (AA) with 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH). Obtained resultants indicated that the adduct 3FMBA-DPPH shows highest binding constant and highest binding free energy (2.28×10-5 M -1 ) and (-30.59 KJmol -1 ) respectively. Binding free energy of 3FMAB-DPPH is almost twice greater than that of the adducts 2FMAB-DPPH (-14.47 KJmol -1 ) and also is greater than the adduct formed between the standard antioxidant ascorbic acid and 1,1-diphenyl-2-picrylhydrazyl radicals (AA-DPPH) ).

Solvent extraction effects on phytochemical profiles, antioxidant activities and superoxide anion radical interactions of whole fruit, pulp and skin of Cucurbita pepo L.

“…The free radical scavenging capacity of extracts were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), as described earlier with some modifications [14,15,30]. 1,5 ml of different concentration (1, 2 and 3 mg/ml) of sample solutions was mixed with 1.5 ml of DPPH solution (2 mg of DPPH were dissolved in 50 ml of methanol).…”

“…the tubes were incubated in the dark. After 30 minutes, the absorbance was read at 517 nm [14,15,31]. The percentage of scavenged DPPH was calculated using the following equation 2:…”

“…Scavenging activity of DPPH is based on one-electron reduction which represents the free radical reducing activity of antioxidants [14,15,35]. Lower IC50 values thus indicate a higher DPPH free radical scavenging activity.…”

“…the present work was aimed to evaluate, for the first time the effects of extraction solvents on total phenol content, total flavonoid content, and antioxidant activity of whole fruit, pulp and skin of Cucurbita pepo L. Two different antioxidant assays were chosen for the measurement of antioxidant function in samples and compared : DPPH• radical scavenging and total antioxidant activity [14,15]. For the second time cyclic voltammetry method was used to evaluate the interaction between extracts of whole fruit, pulp, skin and superoxide anion radical (…”

A Molecular Docking Study of N-Ferrocenylmethylnitroanilines as Potential Anticancer Drugs

“…Ferrocene derivatives has drawn interest due to their stable chemical structure and large variety of biological properties [3][4][5][6][7][8][9][10][11][12][13][14], ferrocenes incorporated heterocyclic ring systems have a huge potential to become successful drug candidates which was proven in the recent past few years [15][16]. Ferrocenylmethylnitroanilines exhibit diverse biological activities such as antioxidant activities [17]. Molecular Docking is the computational modelling of the structure of complexes formed by two or more interacting molecules.…”