Determination of Bioaccumulation Ratios and Antioxidant Activity of Lead and (+)-Catechin in Some Rat Tissues After Co-Administration

Abstract

Investigate the effects of the (+)-Catechin (CTH) against the toxic Lead (Pb) accumulationrelated oxidative stress in rat liver and also of bioaccumulation ratios in some rat tissues Sprague-Dawley female rats were used. I. Control group: received (0.5 ml/kg 0.9% saline) II. Pb intoxicated group: received (20 mg/kg Pb) III. CTH supplemented group: received (20 mg/kg Pb) + (50 mg/kg CTH). All doses were given intragastrically (i.g) and daily for 15 days. All animals were dissected after two weeks of treatment. Pb, levels were determined in the colon, liver, stomach, pancreas, bladder, heart, kidney, and brain tissue by inductively coupled plasma-optical emission spectrophotometer (ICP-OES 720 ES), after acid digestion in all samples. The superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) levels were determined spectrophotometrically. Results were evaluated statistically. In the study, the most lead accumulation was determined in the bladder and the least accumulation, respectively, colon, stomach, liver, kidney, brain, pancreas, and heart. We found that CTH treatment increase liver CAT levels. We concluded that CTH may be partially useful in Pb removal in some of the rat tissues.