Determination of Biochemical Composition and Pigment Content in Legume and Cereal Microgreens

Abstract

Background: Legumes and cereals microgreens are consumed both in healthy nutrition and as a natural food against many degenerative diseases due to their rich bioactive content. The research was carried out to determine the biochemical contents of 5 legumes and 7 cereals. Methods: The research was conducted in Van Yuzuncu Yıl University Field Crops Department Laboratory in 2020, in a controlled growing environment, according to the completely randomized experimental design. Total antioxidant activity, total phenolic, total flavonoid and total ascorbic acid contents and total Chlorophyll, Chlorophyll a, Chlorophyll b and Carotenoid contents and correlation between them were determined. Result: The differences between the means of all traits were found to be significant. The biochemical contents of cereals were 1.6-7 times higher than legumes. There was no big difference between the pigment values. In legumes, Bilensoy alfalfa had the highest total antioxidat activity and total flavonoid content, Uzbek lentil total phenolic content and Goynuk bean had the highest Total ascorbic acid. In cereals, Kirklar oat had the highest total antioxidat activity, total phenolic, total flavonoid and total ascorbic acid contents, while Larende barley had the lowest values (except total ascorbic acid content). While total Chlorophyll and Chlorophyll a amounts were highest in Kirklar barley and lowest in Uzbek lentils, the opposite situation occurred in Chlorophyll b and Carotenoid amounts. There was no significant correlation between biochemical parameters in legumes. There was a positive and significant correlation between total phenolic content with total antioxidant activity and between total ascorbic acid with total fenolic content in cereals. There were positive and significant correlations between all pigment parameters in both legumes and cereals.

Morphological, Molecular, and Biochemical Characterization of a Unique Lentil (Lens culinaris Medik.) Genotype Showing Seed-Coat Color Anomalies Due to Altered Anthocyanin Pathway

“…Similarly, in the 6-day old lentil sprouts, nearly similar TPC (19.4 mg/g DW) was reported by Swieca et al [ 52 ]. Furthermore, in the lentil seeds, Fouad et al [ 53 ] and Altuner [ 54 ] reported TPC to the tune of 1510.10 and 385.44 mg/100 g DW, respectively. A similar range of TPC was also reported for mustard (1889.76 mg/100 g FW) and radish (211.19 mg/100 g FW) seedlings [ 55 ].…”

Prospects of microgreens as budding living functional food: Breeding and biofortification through OMICS and other approaches for nutritional security

“…Thus, it was concluded that total phenolic contents (TPC) was considerable good in red clover (46%) and maize (73%) as compared to cowpea and other studied legumes ( Altuner et al, 2022 ). Although, biochemical parameters have not been correlated in legumes but pigment parameters were positively associated in legumes and cereals ( Altuner, 2021 ). Further, positive correlation was found for total antioxidant activity, total phenolic content and total ascorbic acid in cereals ( Altuner, 2021 ).…”

“…Although, biochemical parameters have not been correlated in legumes but pigment parameters were positively associated in legumes and cereals ( Altuner, 2021 ). Further, positive correlation was found for total antioxidant activity, total phenolic content and total ascorbic acid in cereals ( Altuner, 2021 ).…”