The design and construction of anaerobic reactors for a wide range of substrates from one or more sources require a good understanding of each substrate's physical and biochemical characteristics. Tests of biological or biochemical methane potential (BMP), i.e., anaerobic biodegradability analyses, are used to determine anaerobic biodegradability and total methane potential of waste or biomass, as well as the velocity of the process in laboratory conditions. This paper presents the use of an automated methane production potential testing system (AMPTS). AMPTS was developed for online measurements of ultra-low flows of biogas and bio-methane produced from the anaerobic fermentation of any biodegradable substrate (both solid and liquid form) at the laboratory level. Specific measurements and analysis of the results were performed for different raw materials: sludge from treated wastewater, animal manure, corn silage, straw, and their combinations.