Determination of biological markers for alcohol abuse

Mußhoff, Frank; Daldrup, Th.

doi:10.1016/s0378-4347(97)00503-3

Cited by 60 publications

(36 citation statements)

References 247 publications

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“…6B) and apparent half lifes were estimated to be 7.24 and 6.74 days, respectively. These values compare well with those reported in the literature [1][2][3][4]31].…”

Section: Long-term Cdt Monitoringsupporting

confidence: 91%

“…Therefore, laboratory markers are strongly required as diagnostic tools for an early and more reliable detection of excessive alcohol consumption and alcoholism. A big variety of compounds have been investigated and some of them like g-glutamyl transferase, aspartate aminotransferase, alanine aminotransferase, and mean corpuscular volume are widely used in the clinical practice, however, with questionable sensitivities and/or specificities [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

“…In the last two decades, different analytical approaches for the quantitative determination of CDT in human serum have been developed, including isoelectric focusing, anion-exchange chromatography followed by immunochemical determinations, high-performance liquid chromatography (HPLC), and capillary zone electrophoresis (CZE) [1,2,4,[10][11][12]. With the adoption of dynamic capillary coatings, preventing proteins from adsorbing to the capillary surface, CZE in fused-silica capillaries turned out to be an effective analytical approach for the separation and quantitative determination of the Tf isoforms and thus CDT [2,[13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Improved capillary electrophoresis method for the determination of carbohydrate‐deficient transferrin in patient sera

et al. 2004

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Capillary zone electrophoresis (CZE) with a dynamic double coating formed by charged polymeric reagents represents an effective tool for the separation of iron-saturated transferrin (Tf) isoforms and thus the determination of carbohydrate-deficient transferrin (CDT, sum of asialo-, monosialo- and disialo-Tf in relation to total Tf) in human serum. Using the CEofix-CDT reagents, a 50 microm inner diameter (ID) capillary of 60 cm total length and the P/ACE MDQ under optimized instrumental conditions (20 kV and 30 degrees C) is demonstrated to provide outstanding assay precision for the determination of CDT in human serum. For CDT levels of 1.0% and 4.5%, precision relative standard deviation (RSD) values (n = 8) were determined to be < 3.0% and < 1.5%, respectively. During the first year of operation under routine conditions, more than 600 patient samples were analyzed in a total of 62 sets of runs. Except for selected samples of patients with severe liver diseases, interference-free Tf patterns were detected. Asialo-Tf was not detected in control sera and in patient sera with a CDT level < 1.70%, but became detectable in 89.6% of sera with > 2.3% disialo-Tf. Monosialo-Tf was only detected in two sera containing > 13.3% CDT. The optimized CZE assay was applied to confirm positive CDT results produced by an immunoassay during long-term monitoring of a patient which led to the determination of the elimination kinetics of asialo-Tf, disialo-Tf, and CDT after an episode of high alcohol consumption (estimated apparent half lifes of 4.86, 7.24, and 6.74 days, respectively). The optimized CZE assay with an upper reference limit for CDT of 1.70% represents an attractive alternative to high-performance liquid chromatography (HPLC). It features simpler sample preparation, faster analysis time, and higher isoform resolution compared to the most recent HPLC approach and can thus be regarded as a new candidate of a reference method for CDT.

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“…6B) and apparent half lifes were estimated to be 7.24 and 6.74 days, respectively. These values compare well with those reported in the literature [1][2][3][4]31].…”

Section: Long-term Cdt Monitoringsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improved capillary electrophoresis method for the determination of carbohydrate‐deficient transferrin in patient sera

et al. 2004

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“…Un modèle plus complexe inclut 10 paramètres : chlore, sodium, rapport bilirubine directe/totale, urée, HDL, monocytes, phosphore plaquette, ASAT et hémoglobi-ne (34). Ce modèle permet de distinguer 98 % des sujets ayant une forte consommation et 95 % des sujets à consommation plus faible (33).…”

Section: La Transferrine Désialyléeunclassified

“…Ce modèle permet de distinguer 98 % des sujets ayant une forte consommation et 95 % des sujets à consommation plus faible (33). D'autres marqueurs ont également été décrits, comme les adduits à l'acétaldéhyde, les esters éthyliques des acides gras, les congénères, mais ils ne sont pas encore utilisés en routine (34). D'autre part, l'analyse d'une autre matrice, comme les cheveux peut aussi permettre d'apprécier dans le temps les consommations en alcool, en mesurant l'éthylglucuronide ou encore les esters éthyliques des acides gras (35).…”

Section: La Transferrine Désialyléeunclassified