Determination of Bisphenol a in Beverages by Rp-HPLC

Abstract

Bisphenol A (BPA) is a monomer widely used in the production of polycarbonate, epoxy resins, diacrylates and phenolic resins. A small quantity of BPA can migrate into the food and thus it can be potential hazard for human health and environment. Therefore, quantitative determination of BPA is of a great importance. A fast, simple, precise and economic RP-HPLC method with UV-DAD detection for quantitative determination of BPA in beverages was developed. Three different analytical columns were tested: Hypersil ODS (250 mm x 4.6 mm; 5 µm), LiChrospher 60 RP-Select B (125 mm x 4 mm; 5 µm) and Purospher ® STAR RP-18 endcapped (30 mm x 4 mm; 3 µm). Analyzed beverages were packed in plastic bottles and small glass bottles closed with a cops coated with epoxy resin on inside. For quantitative determination of BPA following experimental conditions were established: mobile phase consisted of acetonitrile/water 50/50 (v/v), flow rate of 1 mL/min, column temperature of 25 o С, injection volume of 5 µL and UV detection at 200 nm. The method was developed in an isocratic manner and with a reversed phase column. Prior the analyses the samples were filtrated through syringe filters Spartan-T with pore size 0.45 µm. The following parameters were determined: retention time, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, selectivity and sensitivity. The RP-HPLC method with UV-DAD detection can be successfully used for quantitative determination of BPA in nonalcoholic beverages without pre-treatment. The BPA was not detected in the analyzed beverages.

Analysis of Bisphenol A migration from microwaveable polycarbonate cups into coffee during microwave heating

“…All the samples were filtered through syringe filters with 0.45 μm pore size before high‐performance liquid chromatography (HPLC) analysis (Cao & Ji, 2013; Petanovska‐Ilievska et al ., 2019). Polytetrafluoroethylene (PTFE) syringe filters adsorb the least amount of BPA, and pore size of 0.2 μm gives around 95% detection rate of BPA in the filtrate (Godby & Conklin, 2017).…”

“…There are different techniques that are frequently reported in the literature to determine BPA migration. Due to the complexity of food matrix and presence of BPA in low concentrations, BPA can only be eluted accurately with advanced chromatographic methods (Petanovska‐Ilievska et al ., 2019). The major analytical methods extensively reported in the literature to detect BPA include are high‐performance liquid chromatography (HPLC), ultra‐performance liquid chromatography (UPLC), liquid chromatography with tandem mass spectrometry (LC‐MS‐MS) and gas chromatography (GC) (Szymański et al ., 2006; Maragou et al ., 2008; Lim et al ., 2009, Cao & Ji, 2013; Benecyo, 2016).…”

Bisphenol A analysis and quantification inconsistencies via HPLC-UV: a systematic review with technical notes

“…12.73% of the analyzed papers studied the leaching of BPA from consumer products, mostly dentistry resins [31,[46][47][48][49][50][51][52], but also from plastic packaging and bottles [53,54]. The migration of BPA from food packaging could lead to food contamination, but only a few studies (3.64%) directly investigated this phenomenon [55,56]. In addition, 5.45% of the studies were mainly directed to health risks of BPA in humans, quantifying the pollutant from human biological specimens (e.g., saliva, blood, breast milk) [47,[57][58][59], and only a few papers were designed for the investigation of BPA effects on plants or animal models [60,61], (Fig.…”

“…B Overlapped main purposes with other secondary objectives of the analyzed papers [64] or by air pollution near polycarbonate molding plants [65], bee pollen [66], mussels [67] and brine shrimp [68]. Food contamination was determined in meat samples [53], beverages [55,69,70], milk [59,63,71,72], oils [56,63], and canned food products [59,71,73] with most studies revealing that BPA could be leached from the plastic can linings, packaging, and bottles [53,55,56,71,[74][75][76].…”