Determination of Blood Total, Reduced, and Oxidized Glutathione in Pediatric Subjects

Pastore, Anna; Piemonte, Fiorella; Locatelli, Mattia; Russo, Anna Lo; Gaeta, Laura; Tozzi, Giulia; Federici, Giorgio

doi:10.1093/clinchem/47.8.1467

Cited by 183 publications

(85 citation statements)

References 7 publications

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“…Pastore et al [21] reported SOD catalyzes the dismutation of superoxide to hydrogen peroxide and oxygen, thereby reducing the likelihood of superoxide anion reacting with nitric oxide to form reactive peroxynitrite [59]. The increased serum activities of catalase and SOD as observed in this study suggest that the Aframomum melegueta [Roscoe] K. Schum extract has an in vivo antioxidant activity and is capable of ameliorating the effect of ROS in biologic system [59].…”

Section: Glutathione Peroxidise (Gpx)supporting

confidence: 49%

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Assessment of in-vitro Antioxidant/Enzymes Inhibitory Potentials of Aframomum melegueta [Roscoe] K. Schum (Grains of Paradise) Leaf, Stem Bark, Seed Bark and Seed Extracts

Osuntokun

Olumekun

Ajayi

et al. 2020

ACRI

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The purpose of this research work is to evaluates the in-vitro antioxidant and antioxidant enzymes inhibition potentials of Aframomum melegueta [Roscoe] K. Schum (Grains of Paradise) Leaf, Stem Bark, Seed Bark and Seed Extracts. Aframomum melegueta is a spice with a similar composition as Ginger, that belongs to the same Zingiberaceae family, used in Nigeria, West Africa and it is used for the treatment of infectious diseases such as urinary tract infections, cancer and diabetes. Antioxidants neutralize the effect of free radicals through different ways and may prevent the body from various diseases. The plant extract were collected from a rain forest in Akugba Akoko, Ondo state, Nigeria. The plant sample was dried, pulverized, filtered with Whatman No 1 filtered paper and the filtrates was concentrated in-vacuum using vacuum rotary evaporator at 40°C and was later concentrated to dryness in a hot-air oven at 40°C. Assessment of antioxidant potentials were performed using Singleton method Total flavonoids (mg/g), Phenol (mg/g), Ferric reducing (FRAP) potentials (mg/g), Free radical scavenging ability (DPPH) (1, 1- diphenyl-2-picryhydrazyl) potentials (%),2,2’-azino-bis(3-ethylbenthiazoline-6-sulphonic acid), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging potentials (mMol/g), Fe2+ chelation potentials (%) and OH Radical Scavenging potentials (%) were evaluated. Antioxidant enzymes of plant were assessed using Bentller method, Superoxide Dismutase (SOD) (%), Reduced Glutathione (GSH) (%), Catalase (CAT) and Glutathione Peroxidise (GPX) were evaluated. Seed extract among other extracts of Aframomum melegueta has the highest quantity of flavonoids, Phenol, FRAP, DPPH, ABTS, Fe2+ chelation and OH radical scavenging potential. The leaf extract of A. meleguata has the highest percentage of Superoxide Dismutase (SOD) and Catalase (CAT) while stem bark of Aframomum melegueta has the highest percentage of Reduced Glutathione (GSH) and Glutathione Peroxidise (GPX).it can be deduced from this research work that all parts of the A. melegueta has pharmacological and therapeutic activities judging from literature that A. melegueta can be used to prevent oxidative damage by ROS (Reactive oxygen species) reacting with free radical chelating and catalytic metals which can be used to reduce and prevent various diseases like heart disease, cancer, DNA degeneration, pulmonary and neurological disorder. SOD, GPX, GSH help to control cytokins induced peroxidise level and mediate signal transduction in mammalian cells. It can be deduced that Aframomum melegueta should be the focal point in human diet, natural herbal drug supplement and even in addition to conventional drug to improve the potency and quality of natural drug system. The use of Aframomum melegueta should be encouraged. These results show the potential of Aframomum melegueta as a source of bioactive compounds to be used for pharmaceutical, nutraceutical, and cosmeceutical applications.

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Section: Glutathione Peroxidise (Gpx)supporting

confidence: 49%

“…Glyoxalase I (EC 4.4.1.5) catalyzes the conversion of methylglyoxal and reduced glutathione to S-Dlactoyl-glutathione. Glyoxalase II (EC 3.1.2.6) catalyzes the hydrolysis of S-D-lactoylglutathione to glutathione and D-lactic acid [21].…”

Section: Introductionmentioning

confidence: 99%

Assessment of in-vitro Antioxidant/Enzymes Inhibitory Potentials of Aframomum melegueta [Roscoe] K. Schum (Grains of Paradise) Leaf, Stem Bark, Seed Bark and Seed Extracts

Osuntokun

Olumekun

Ajayi

et al. 2020

ACRI

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“…Glutathione (g-L-glutamyl-L-cysteinylglycine) is one of the major soluble, low molecular weight antioxidants in plant cells (Airaki et al, 2011). The intracellular concentration of glutathione is an indicator of oxidative stress in cells (Pastore et al, 2001). Within cells, glutathione exists in reduced (GSH) and oxidized (GSSG) states.…”

Section: Erastin Triggers Ros and Fe 3+ Accumulation And Glutathione mentioning

confidence: 99%

Iron- and Reactive Oxygen Species-Dependent Ferroptotic Cell Death in Rice-Magnaporthe oryzae Interactions

et al. 2018

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Hypersensitive response (HR) cell death is the most effective plant immune response restricting fungal pathogen invasion. Here, we report that incompatible rice (Oryza sativa) Magnaporthe oryzae interactions induce iron-and reactive oxygen species (ROS)-dependent ferroptotic cell death in rice cells. Ferric ions and ROS (i.e., H 2 O 2 ) accumulated in tissues undergoing HR cell death of rice leaf sheath tissues during avirulent M. oryzae infection. By contrast, iron did not accumulate in rice cells during virulent M. oryzae infection or treatment with the fungal elicitor chitin. Avirulent M. oryzae infection in DOsnadp-me2-3 mutant rice did not trigger iron and ROS accumulation and suppressed HR cell death, suggesting that NADPmalic enzyme2 is required for ferroptotic cell death in rice. The small-molecule ferroptosis inhibitors deferoxamine, ferrostatin-1, and cytochalasin E and the NADPH oxidase inhibitor diphenyleneiodonium suppressed iron-dependent ROS accumulation and lipid peroxidation to completely attenuate HR cell death in rice sheaths during avirulent M. oryzae infection. By contrast, the small-molecule inducer erastin triggered iron-dependent ROS accumulation and glutathione depletion, which ultimately led to HR cell death in rice in response to virulent M. oryzae. These combined results demonstrate that iron-and ROS-dependent signaling cascades are involved in the ferroptotic cell death pathway in rice to disrupt M. oryzae infection.

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“…GSH is one of the most important members of the antioxidant defense mechanism. It provides reducing capacity for several reactions and plays an important role in intraperoxisomal detoxification of ROS, hydrogen peroxide and other peroxides (Pastore et al 2001). In the present study, VPA treatment did not change GSH levels significantly.…”

Section: Discussionmentioning

confidence: 99%

Effects of Chard (Beta Vulgaris L. Var. Cicla) on Cardiac Damage in Valproic Acid-Induced Toxicity

Üstündağ

Tunalı

Alev

et al. 2015

Journal of Food Biochemistry

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The aim of this study was to examine the effects of chard on valproic acid (VPA)-induced cardiac damage. Female Sprague-Dawley rats were grouped as control, chard given control (100 mg/kg/day, by gavage), VPA (500 mg/kg/day, intraperitoneally) and chard given VPA (100 mg/kg/day chard by gavage, 500 mg/kg/day VPA, intraperitoneally). The aqueous extracts of chard leaves were given 1 h prior to administration of VPA for 7 days. Malondialdehyde (MDA), total sialic acid (SA) levels and catalase (CAT) activity significantly increased in the VPA group compared with the control group (P < 0.05). Chard administration significantly decreased MDA and SA levels in the control and in the VPA groups (P < 0.05). Chard administration also significantly increased CAT activities and glutathione levels both in the control and in the VPA groups (P < 0.05). As a conclusion, chard consumption may prevent cardiac tissue from oxidative stress and inflammation in VPA-induced toxicity.

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Determination of Blood Total, Reduced, and Oxidized Glutathione in Pediatric Subjects

Cited by 183 publications

References 7 publications

Assessment of in-vitro Antioxidant/Enzymes Inhibitory Potentials of Aframomum melegueta [Roscoe] K. Schum (Grains of Paradise) Leaf, Stem Bark, Seed Bark and Seed Extracts

Assessment of in-vitro Antioxidant/Enzymes Inhibitory Potentials of Aframomum melegueta [Roscoe] K. Schum (Grains of Paradise) Leaf, Stem Bark, Seed Bark and Seed Extracts

Iron- and Reactive Oxygen Species-Dependent Ferroptotic Cell Death in Rice-Magnaporthe oryzae Interactions

Effects of Chard (Beta Vulgaris L. Var. Cicla) on Cardiac Damage in Valproic Acid-Induced Toxicity

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