Determination of by-products formed during the ethanolic fermentation, using batch and immobilized cell systems of Zymomonas mobilis and Saccharomyces bayanus

Amin, G.; Eynde, Erik Van; Verachtert, Hubert

doi:10.1007/bf00508121

Cited by 64 publications

(22 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CSL contains lactic acid, and it is known that lactic acid is produced, depending on fermentation conditions, as a byproduct by Zymomonas mobilis in fermentation broths. 17,20,29 Carboxylic acids dissociate into their ionic species and H + in aqueous solutions, and the degree of dissociation of carboxylic acids depends on the pH of the bulk solution. 30 Therefore, the adsorption behaviour of lactic acid on the membrane material, silicalite powder, was investigated.…”

Section: Effects Of Components In Fermentation Broths On Pervaporatiomentioning

confidence: 99%

“…13 For practical use, the deterioration in pervaporation performance in the separation of ethanol fermentation broths should be resolved. 14 We previously reported that the pervaporation performance of silicalite membranes is greatly affected by compounds in aqueous ethanol solutions, 15,16 and that the adsorption of succinic acid (a byproduct of a yeast fermentation) 17 onto silicalite crystals causes the drastic deterioration in pervaporation performance. 16 In order to avoid the deterioration in pervaporation performance caused by succinic acid, it is necessary both to coat silicalite membranes with silicone rubber, and to adjust the pH of ternary ethanol/water/succinic acid mixtures to above 5.…”

Section: Introductionmentioning

confidence: 99%

“…Fortunately, Zymomonas mobilis does not produce succinic acid as a byproduct during ethanol fermentation. 17 This indicates that fermentation broths by Zymomonas mobilis would be more suitable than those by yeasts for pervaporation of bioethanol using silicalite membranes.…”

mentioning

confidence: 97%

See 2 more Smart Citations

Stabilized production of highly concentrated bioethanol from fermentation broths by Zymomonas mobilis by pervaporation using silicone rubber‐coated silicalite membranes

Ikegami

Negishi

Yanase

et al. 2007

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

Since pervaporation performance of ethanol-permselective silicalite membrane, which is an aluminumfree hydrophobic zeolite, in the separation of fermentation broths by yeast are negatively affected by succinic acid, the potential of pervaporation using silicone rubber-coated silicalite membranes of ethanol fermentation broths, not containing succinic acid, by Zymomonas mobilis was investigated for the reliable production of concentrated bioethanol. In the separation of fermentation broths, the pervaporation performance was influenced by nutrients used for the preparation of fermentation broths. In the separation of a broth prepared with yeast extract, pervaporation performance was greatly compromised by accumulation of a substance(s) having an ultraviolet absorption maximum at approximately 260 nm not only in total flux, but also in permeate ethanol concentration compared to the separation of binary ethanol/water mixtures. When supplying a prepared broth with corn steep liquor without the accumulation of a substance(s) having an ultraviolet absorption maximum at approximately 260 nm, the permeate ethanol concentration did not decrease. Treating the prepared broth with activated carbon was effective in restraining the decrease in total flux. Pervaporation performance is also deteriorated by the adsorption of lactic acid contained in corn steep liquor onto the silicalite crystals. In the separation of ternary mixtures of ethanol/water/lactic acid, accomplished by adjusting the ternary mixtures to pH > 5, more than 90% of the permeation flux in the separation of binary ethanol/water mixtures was obtained, and the permeate ethanol concentration was comparable to that obtained in the separation of binary mixtures. For stably performing pervaporation, it is important to prepare ethanol fermentation broths by Zymomonas mobilis in which lactic acid concentration is as low as possible.

show abstract

Section: Effects Of Components In Fermentation Broths On Pervaporatiomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Stabilized production of highly concentrated bioethanol from fermentation broths by Zymomonas mobilis by pervaporation using silicone rubber‐coated silicalite membranes

Ikegami

Negishi

Yanase

et al. 2007

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…The productivity, expressed as grams of ethanol produced per hour per unit of fermentation volume, can be increased by optimizing the composition of the culture medium, by the selection of an appropriate microorganism strain or through the adaptation of the design of reactors [7]. One challenge today is to reduce ethanol production costs, and an alternative is to reduce the cost of the culture media, which can represent about 30% of the inal production costs of ethanol [8].…”

Section: Tecnología De Inmovilizaciónmentioning

confidence: 99%

“…Other studies have been developed with immobilized algal cells to remove nutrients (N and P) from wastewater, phenol and hexavalent chromium [14][15][16][17]. Similarly, the immobilization of Zymomonas and Saccharomyces have been used for the bio-ethanol production from waste materials [7][8][9][10][18][19][20].…”

Section: Tecnología De Inmovilizaciónmentioning

confidence: 99%

Potential Production of Ethanol by Saccharomyces cerevisiae Immobilized and Coimmobilized with Zymomonas mobilis: Alternative for the Reuse of a Waste Organic

Ruiz-Marín¹,

Lopez²,

Narváez-García³

et al. 2017

Yeast - Industrial Applications

View full text Add to dashboard Cite

Fermentation technologies have been developed to improve the production of ethanol and an alternative is the immobilization technology, which ofers the possibility of eiciently incorporating symbiotic bacteria in the same matrix. This study analyzes the potential use of immobilized and coinmobilized systems on beads of calcium alginate for ethanol production used mango waste (Mangifera indica) by Zymomonas mobilis and Saccharomyces cerevisiae compared with free cells culture and evaluate the efect of glucose concentration on productivity in coimmobilized system using a Chemostat reactor Ommi Culture Plus. For free cell culture, the productivity was higher for Z. mobilis ). The conversion of glucose to ethanol for coimmobilized system was higher (6.91 mol ethanol) with 50 g L -1 of glucose compared to 200 g L -1 of glucose (5.82 mol ethanol); suggesting the immobilized and coimmobilized cultures compared with free cells ofer an opportunity for the reuse of organic residues and high alcohol production.

show abstract

Gas chromatographic analysis of free fatty acids. Part 1. Principles and methodic variants

Pörschmann¹,

Welsch

Pörschmann

1987

Acta Biotechnologica

View full text Add to dashboard Cite

Proceeding from a literature search the problems faced by the today's gas chromatographer concerned with analysis of free fatty acids are summarized. In terms of column technology selfmade OV-l/FFAP mixed phase capillary columns are well suited for adequate tailing -free elution of these polar molecules. Whereas fatty acids having two and more carbon atoms can be analyzed in an underivatized state on acidic capillary columns, the involving of formic acid and dicarboxylic acids calls for deactivation procedures.

show abstract

Determination of by-products formed during the ethanolic fermentation, using batch and immobilized cell systems of Zymomonas mobilis and Saccharomyces bayanus

Cited by 64 publications

References 7 publications

Stabilized production of highly concentrated bioethanol from fermentation broths by Zymomonas mobilis by pervaporation using silicone rubber‐coated silicalite membranes

Stabilized production of highly concentrated bioethanol from fermentation broths by Zymomonas mobilis by pervaporation using silicone rubber‐coated silicalite membranes

Potential Production of Ethanol by Saccharomyces cerevisiae Immobilized and Coimmobilized with Zymomonas mobilis: Alternative for the Reuse of a Waste Organic

Gas chromatographic analysis of free fatty acids. Part 1. Principles and methodic variants

Contact Info

Product

Resources

About