Determination of Cadmium–Metallothioneins in Zebra Mussels Exposed to Subchronic Concentrations of Cd2+

Tessier, Catherine; Blais, Jean-Simon

doi:10.1006/eesa.1996.0031

Cited by 16 publications

(8 citation statements)

References 4 publications

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“…Zebra mussels are capable of concentrating Cd in their tissues under laboratory exposure conditions [9, 24‐26]. Various freshwater locations in Québec and Ontario have been sampled for free Cd 2+ concentrations in overlying waters, with measured values ranging from 2 ng/L to 1.58 μg/L Cd [27], which are similar to values obtained in laboratory exposures of these mussels [24–26].…”

Section: Discussionmentioning

confidence: 80%

“…It may also represent a MT dimer. Dimers of MT have been observed by gel‐filtration chromatography of oyster MT [14] and suggested in crude extracts of zebra mussels [9] and other freshwater bivalves [15]. Amino acid compositions characteristic of MTs have been determined for three major anion‐exchange isoforms of MT dimers purified from the marine mussel Mytilus edulis [16].…”

Section: Discussionmentioning

confidence: 99%

“…Approximately 1,000 mussels were transferred to an aquarium tank containing 10 L of continuously aerated tap water (containing <0.1 μg/L Cd) maintained at 12°C. Water was changed every day, and 0.36 g Chlorella vulgaris algae (ACTA International, Sunnyvale, CA, USA) was added to the fresh, aerated water as a source of food for the mussels [9]. After an initial acclimatization period of 1 month, mussels were exposed to 500 μg/L Cd (CdCl 2 ) (J.T.…”

Section: Methodsmentioning

confidence: 99%

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Characterization of metallothionein‐like proteins from zebra mussels (Dreissena polymorpha)

High

Barthet

McLaren

et al. 1997

Enviro Toxic and Chemistry

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Abstract-Zebra mussels (Dreissena polymorpha) are freshwater mollusks that have recently infested the Great Lakes ecosystem. Possessing a large capacity for filtration, these mussel populations act as bioconcentrators for contaminants, such as heavy metals, found in the Great Lakes ecosystem. Metallothionein is a low-molecular-weight, heavy metal-binding protein found in most living organisms. Characterization and partial purification of metallothionein-like Cd-binding proteins from zebra mussels were performed. Zebra mussels were exposed to 500 g/L Cd for 14 d. During the exposure period, two mussels were removed on alternate days for analysis of Cd-binding proteins. Gel-filtration high-performance liquid chromatography-microatomization-atomic absorption spectrophotometry results showed a single Cd-binding molecular weight protein fraction after 2 d of Cd exposure. After 10 d of Cd exposure, however, mussels exhibited an additional, higher molecular weight, Cd-binding protein fraction. The lower molecular weight metallothionein-like Cd-binding protein was further isolated and purified by acetone fractionation, Sephadex G75, and diethylaminoethyl anion-exchange chromatography. The quantities of Zn, Cu, and Cd in the anion-exchange metallothionein-like protein isoforms were determined by inductively coupled plasma-mass spectrometry. The ability to bioconcentrate heavy metals in a metallothionein-like form coupled with their large population in the Great Lakes make zebra mussels suitable for use in a freshwater biomonitoring program for aquatic metal contamination.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Characterization of metallothionein‐like proteins from zebra mussels (Dreissena polymorpha)

High

Barthet

McLaren

et al. 1997

Enviro Toxic and Chemistry

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“…Metallothionens generally have a molecular weight of about 6 to 7 kDa, but the 23‐kDa protein may represent a polymer. Dimers of MT have been inferred from GPC results in several other studies [30,31]. The fact that the 23 kDa estimate obtained by GPC is higher than the 17 kDa estimate obtained by SDS‐PAGE and Western blot may be a consequence of the rod shape of metallothioneins (causing a metallothionein to migrate in gelpermeation as would be expected from a larger‐sized spherical protein).…”

Section: Discussionmentioning

confidence: 94%

Metallothionein‐like protein in the least killifish Heterandria formosa and its role in cadmium resistance

Xie¹,

Klerks²

2004

Enviro Toxic and Chemistry

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Metallothioneins (MTs) are a class of low molecular weight, cysteine-rich, metal-binding proteins ubiquitous in animals. They function in metal regulation and detoxification. An MT-like protein was separated by gel-permeation high-performance liquid chromatography (HPLC) in the least killifish Heterandria formosa exposed to 6 mg/L of Cd for 26 h. Western blot analysis showed that this protein cross-reacted with a cod-MT antibody. We quantified MT-like protein levels by the Cd-hemoglobin saturation assay in fish from two Cd-resistant and two control laboratory populations. Fish from the resistant populations had higher MT-like protein levels than those from the control populations; this difference was found for basal levels and for levels after 26 h of exposure to Cd. Our results suggest that the MT-like protein detoxified Cd in the least killifish and accounted for at least part of the increased resistance in the Cd-resistant laboratory populations.

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“…Following the development of methods for MT quanti"cation (Bebianno and Langston, 1989;Scheuhammer and Cherian, 1991;Tessier and Blais, 1996;Viarengo et al, 1997), induction of MT or MT-like proteins has been reported extensively in many marine invertebrates, especially in common biomonitors such as Mytilus galloprovincialis (Viarengo et al, 1985;Bebianno and Machado, 1997), Mytilus edulis (Bebianno and Langston, 1991),¸ittorina littorea (Bebianno et al, 1992), Ostrea edulis (Alonso and Maetin-Mateo, 1996), Dreissena polymorpha (Tessier and Blais, 1996), and Macoma balthica (Bordin et al, 1997). A recent "eld study using M. galloprovincialis as biomonitor has also demonstrated that measurements of MT can provide an accurate indication of subtle environment increases in metal contamination (Bebianno and Machado, 1997).…”

Section: Introductionmentioning

confidence: 99%

Induction of Metallothionein in Dogwhelk Nucella lapillus during and after Exposure to Cadmium

Leung

Furness

1999

Ecotoxicology and Environmental Safety

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Determination of Cadmium–Metallothioneins in Zebra Mussels Exposed to Subchronic Concentrations of Cd2+

Cited by 16 publications

References 4 publications

Characterization of metallothionein‐like proteins from zebra mussels (Dreissena polymorpha)

Characterization of metallothionein‐like proteins from zebra mussels (Dreissena polymorpha)

Metallothionein‐like protein in the least killifish Heterandria formosa and its role in cadmium resistance

Induction of Metallothionein in Dogwhelk Nucella lapillus during and after Exposure to Cadmium

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