Determination of Calpastatin Gene Polymorphism in Kivircik Crossbred Ewes by PCR-RFLP Method

Kaplan, Selçuk; Atalay, Sertaç

doi:10.15316/sjafs.2017.47

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…Three genotypes were identified in the study of the Synthetic population Bulgarian Milk sheep breed with frequencies 0.84, 0.15 and 0.01 for MM, MN and NN, respectively [13]. Similarly, three genotypes with frequencies of 0.82, 0.12 and 0.06 for MM, MN and NN, respectively, were identified at the Soviet merino breed [19], and at Turkish Kıvırcık crossbred ewes with frequencies of 0.82, 0.16 and 0.02, respectively [21]. At Tuvan short fat-tailed sheep breed three genotypes -MM, MN and NN, with similar frequency distribution have been identified (80.39%, 17.65% and 1.96% in steppe type and 77.00%, 22.00% and 1.00% in the mountain type, respectively) [5].…”

Section: Resultsmentioning

confidence: 99%

Polymorphism of CAST gene in five sheep breeds in Bulgaria

et al. 2021

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CAST gene is a candidate marker that influences the intensity of growth and meat quality. The aim of this study was to investigate the genetic variability of CAST gene in five Bulgarian sheep breeds – two merino, two local and one for milk. A total of 150 ewes, belonging to these breeds were investigated for polymorphisms of CAST gene by using PCR-RFLP method. A 622 bp fragment of Ovis aries CAST gene was amplified using PCR. After restriction with endonuclease MspI two alleles were observed in Ascanian, Caucasian, Breznik and Pleven Blackhead breeds. One allele (M) and one genotype (MM) were detected in Cooper-Red Shumen breed. The highest frequency of the allele N was established in the Ascanian merino breed (0.27) followed by Caucasian merino (0.13), Breznik (0.12) and Pleven Blackhead (0.07). Two genotypes - MM and MN, were observed in Caucasian, Breznik and Pleven Blackhead breeds. The three possible genotypes were found only in Ascanian merino ewes - MM, MN and NN. The lowest frequency of the homozygous genotype MM (0.50) and the highest frequency of the heterozygous genotype MN was established in the Ascanian merino breed (0.47). The obtained results confirm that the PCR-RFLP method can be used to identify different genotypic variation of CAST gene in Bulgarian sheep breeds. The established genetic diversity in the calpastatin gene indicates that, after further associative studies, this gene may be included in the breeding programs of certain sheep breeds.

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Section: Resultsmentioning

confidence: 99%

Polymorphism of CAST gene in five sheep breeds in Bulgaria

et al. 2021

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Relationship of Calpastatin Gene Polymorphism with Growth Traits in Some Locally Reared Awassi and Akkaraman Sheep Breeds

Dokgöz,

Özcan

2024

Osmaniye Korkut Ata University Journal of The Institute of Scie

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In this study, it was aimed to determine the polymorphism of Calpastatin gene (CAST) in Awassi and Akkaraman sheep which were reared in Osmaniye province within the scope of National Sheep Breeding Project in the Hand of the Public. The study was carried out on a total of 39 blood samples taken from 20 head Awassi sheep and 19 head Akkaraman sheep reared in 5 different farms. In the study in which PCR-RFLP method was used, 622 bp long PCR amplicons obtained from genomic DNA samples of Awassi and Akkaraman sheep breeds for CAST gene were digested with MspI restriction enzyme; Two bands of 336 and 286 bp in length for homozygous MM allele, a single band of 622 bp in length for homozygous NN allele and three bands of 622, 336 and 286 bp in length for heterozygous MN alleles were observed. The frequencies of M and N alleles in Akkaraman breed were 0.82 and 0.18, respectively, while the frequencies of M and N in Awassi breed were 0.75 and 0.25, respectively. The frequencies of MM, MN and NN genotypes were 0.60, 0.30 and 0.10, respectively. Contrary to Awassi breed, only MM and MN genotypes were observed in Akkaraman sheep and their frequencies were calculated as 0.63 and 0.37, respectively. While deviation in Hardy-Weinberg equilibrium was found to be insignificant in Akkaraman population, it was found to be significant in Awassi sheep. The association analysis revealed that there was a significant association (P

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Determination of Calpastatin Gene Polymorphism in Kivircik Crossbred Ewes by PCR-RFLP Method

Cited by 2 publications

References 16 publications

Polymorphism of CAST gene in five sheep breeds in Bulgaria

Polymorphism of CAST gene in five sheep breeds in Bulgaria

Relationship of Calpastatin Gene Polymorphism with Growth Traits in Some Locally Reared Awassi and Akkaraman Sheep Breeds

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