Determination of camptothecins in DMSO extracts of Nothapodytes foetida by direct injection capillary electrophoresis

Abstract

A rapid capillary electrophoresis procedure was developed for determining the anti-cancer components, camptothecins, in Nothapodytes foetida. The hydrophobic compound was extracted from plant tissue (ca. 1 mL of DMSO for 100 mg of dried plant tissue) with a water-miscible organic solvent, DMSO, at elevated temperature (60 degrees C). The extract was directly injected into the separation capillary (untreated fused silica, 34 cm in length, 75 microm i.d.) and analysed in MEKC mode (369 nm). Within 5 min of migration, camptothecins were successfully separated and quantified by adding organic modifiers to the running buffer (20% DMSO, 90 mm SDS in 10 mm borate buffer, pH 8.60). The linear dynamic range for camptothecin was from 5 to 400 microg/mL. This method was proven to be very suitable for monitoring the amount of camptothecins during the cultivation of the medicinal plant.

Combined use of ionic liquid and sulfobutylether‐β‐cyclodextrin as electrolyte additives for separation and determination of camptothecin alkaloids by CZE

“…The analytes can be separated in citric buffer, but the resolution was unsatisfactory. Thus, in this paper, borate buffer was employed as the running buffer because boron ions can ionize CPT molecule by shifting the intramolecular esterification equilibrium to the carboxylate form . The optimum pH and concentration of the buffer were selected below.…”

CE and CEC analysis of phytochemicals in herbal medicines

“…Ultrasonic extraction was the most frequently used method due to its efficiency and easy manipulation. Indeed, it was employed in about two‐thirds (180 out of 271 articles) of cited references (Supporting Information Tables 1–7) 4–271. Other conventional extraction methods included reflux 6, 14, 15, 21, 45, 74, 84, 87, 97, 117, 121, 123, 131, 134, 157, 162, 164, 169, 171, 172, 176, 179, 182–184, 205, 212, 227, 235, 236, 259, Soxhlet extraction 23, 89, 91, 128, 131, 244, heating extraction 22, 39, 51...…”

“…The influence of several parameters, including buffer type and concentration, pH, additives, voltage, and temperature, has been discussed in our previous reviews 1, 2. Besides, micelle and microemulsion composition for MEKC and MEEKC (Supporting Information Tables 5 and 6) 187–262, as well as sample matrix and injection parameters for online preconcentration 5, 7, 8, 25, 34, 37, 52, 112, 187, 196–198, 207, 209, 220, 238–240, 244, 247 should also be considered.…”

In Vitro Evaluation of Cytotoxic and Genotoxic Effects of Plant Extracts From Nothapodytes Foetida (Wight) Sleumer (Family: Icacinaceae)

“…Earlier reports on phytochemicals and their pharmacological activities of Nothapodytes nimmoniana showed the presence of camptothecin as an alkaloid in different plant parts [8]. 9-methoxy camptothecin is also characterized from Nothapodytes foetida [9] which showed anticancer activity [10]. Previously, a report on camptothecin derived from leaves, bark, and stem bark showed effective action against breast cancer cell line [11].…”