Determination of Capsaicinoids in Habanero Peppers by Chemometric Analysis of UV Spectral Data

Abstract

A novel spectrophotometric method for the determination of capsaicinoids in habanero pepper extracts is described that does not require prior analyte separation. The method uses partial-least-squares (PLS-1) multivariate regression modeling techniques in conjunction with ordinary UV absorption spectral data obtained on alcoholic extracts of habanero peppers (Capsicum chinese). The PLS-1 regression models were developed by correlating the known total concentration of the two major capsaicinoids (capsaicin and dihydrocapsaicin) in the extracts as determined by high-performance liquid chromatography with the spectral data. The regression models were subsequently validated with laboratory-prepared test sets. The validation studies revealed that the root-mean-square error of prediction varied from 4 to 8 ppm, based on the results obtained from models prepared from nine test sets. Once a regression model has been developed and validated, analyses of the extracts can be accomplished rapidly by ordinary spectrophotometric procedures without any prior separation steps.

Physicochemical characterization and bioactive compounds of stalk from hot fruits of Capsicum annuum L.

“…a mixture of seven homologous branched-chain alkyl vanillylamides. Capsaicin and dihydrocapsaicin are the most abundant, while norcapsaicin, nordihydrocapsaicin, nomordihydrocapsaicin, homodihydrocapsaicin and homocapsaicin are found in relative low quantities [4]. Interest in determining and confirming the activities of capsaicin has increased in the last twenty years.…”

Antioxidative effect of Capsicum oleoresins compared with pure capsaicin

“…As a method for measuring the total antioxidant capacity (TAC) of the pure capsaicin or Capsicum extracts was used the in vitro spectroscopic method, FRAP (Ferric Reducing Antioxidant Power) assay [27]. This method is based on the reduction of the Fe 3+ ions to Fe .…”

Metabolomics and Chemometrics as Tools for Chemo(bio)diversity Analysis - Maize Landraces and Propolis

“…This analytical strategy revealed to be fruitful for discrimination of habanero peppers according to their content of capsaicinoids, substances responsible for the pungency of their fruits (Davis et al, 2007).…”