Determination of Carbachol in Aqueous Solution

Puckett, Ramona; Poe, R. D.

doi:10.1002/jps.2600580520

Cited by 8 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, analytical methods are needed to ensure that the concentration of carbachol in pharmaceutical preparations remain at therapeutically active levels. A review of the literature shows that several methods have been developed for the determination of carbachol, including fluorescence, proton magnetic resonance spectroscopy, potentiometry, indirect UV-VIS spectrophotometry, IR spectroscopy, ion chromatography, and the official colorimetric method [26,27,28,29,30,31,32].…”

Section: Introductionmentioning

confidence: 99%

Ecofriendly Long Life Nanocomposite Sensors for Determination of Carbachol in Presence of Choline: Application in Ophthalmic Solutions and Biological Fluids

Al-Harbi

Abdelrahman

El-Kosasy

2019

Sensors

View full text Add to dashboard Cite

Several emerging nano scale forms of carbon are showing great promise in electrochemical sensing such as graphene and multi-walled carbon nanotubes (MWCNTs). Herein we present an ecofriendly method to fabricate long life and sensitive ion selective sensors based on graphene and MWCNTs nanocomposites with no need for volatile organic solvents. Both sensors were fabricated, for the analysis of carbachol in ophthalmic solutions, plasma and urine where ion- association complex was formed between cationic carbachol and anionic Sodium tetra phenyl borate (NaTBP) in a ratio 1:1. Both sensors were evaluated according to the IUPAC recommendation data, revealing linear response in the concentration range 10−7 M to 10−2 M with near Nernstian slopes 50.80 ± 5 and 58.14 ± 3 mV/decade and correlation coefficients 0.9992 and 0.9998 for graphene and MWCNTs based sensors, respectively. Both sensors were successfully applied as stability indicating method for the analysis of carbachol in presence of its metabolite choline, in ophthalmic preparations, in plasma and urine showing good recovery percentage values. MWCNTs based sensor showed some advantages over graphene sensor regarding lower limit of detection (LOD), longer life time and higher selectivity towards carbachol. Statistical comparison of the proposed sensors with the official method showed no significant difference for accuracy and precision.

show abstract

Section: Introductionmentioning

confidence: 99%

Ecofriendly Long Life Nanocomposite Sensors for Determination of Carbachol in Presence of Choline: Application in Ophthalmic Solutions and Biological Fluids

Al-Harbi

Abdelrahman

El-Kosasy

2019

Sensors

View full text Add to dashboard Cite

show abstract

“…Several methods have been developed and utilized toward the detection of carbachol, including IR spectroscopy, 1 H NMR, indirect UV-VIS, fluorescence, and ion-chromatography. [7][8][9][10][11] The indirect UV-VIS has been adopted as the United States Pharmacopeia (USP) monograph (USP 29-NF 24) as the official colorimetric method for the determination of carbachol in ophthalmic solutions. 12 This method relies first on the chlorination of the free N-H group which is then able to oxidize iodide to iodine.…”

mentioning

confidence: 99%

Electrochemical Sensing of Carbachol in Ophthalmic Solutions

Bell

Mousavi

El-Rahman

2018

J. Electrochem. Soc.

View full text Add to dashboard Cite

This paper presents the design and fabrication of a potentiometric sensor for the determination of carbachol in ophthalmic solutions. Carbachol is the active ingredient in certain ophthalmic solutions and its concentration must be maintained to provide its expected therapeutic result during the treatment of glaucoma. Carbachol is an analytically challenging molecule to measure because it lacks a detectable chromophore and is redox-inactive. This work demonstrates the first use of potentiometry to measure carbachol using an ion-selective electrode (ISE). Incorporating calix[4]resorcinarene as an ionophore resulted in a lower limit-of-detection (0.12 μM), larger linear response range (1 μM-10 mM), and enhanced selectivity when compared to the ionophore-free ISE. The ISE was used for direct detection of carbachol in a commercially available ophthalmic solution (without sample separation and pre-treatment steps), and the results were validated using the official US pharmacopeia benchmark method. To expand the application of this sensor, we performed real-time monitoring of carbachol's degradation, allowing for the calculation of the activation energy of its hydrolysis, an important parameter when estimating a drug's shelf-life.

show abstract

High Performance Ion Chromatographic Analysis of Cholinergic Compounds: Carbachol and Bethanechol, and Associated Degradation Products

Trowbridge

2012

Applications of Ion Chromatography for Pharmaceutical and Biological Products

View full text Add to dashboard Cite

Determination of Carbachol in Aqueous Solution

Cited by 8 publications

References 6 publications

Ecofriendly Long Life Nanocomposite Sensors for Determination of Carbachol in Presence of Choline: Application in Ophthalmic Solutions and Biological Fluids

Ecofriendly Long Life Nanocomposite Sensors for Determination of Carbachol in Presence of Choline: Application in Ophthalmic Solutions and Biological Fluids

Electrochemical Sensing of Carbachol in Ophthalmic Solutions

High Performance Ion Chromatographic Analysis of Cholinergic Compounds: Carbachol and Bethanechol, and Associated Degradation Products

Contact Info

Product

Resources

About