Determination of Carbohydrates, Sugar Alcohols, and Glycols in Cell Cultures and Fermentation Broths Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

Hanko, Valoran P.; Rohrer, Jeffrey S.

doi:10.1006/abio.2000.4653

Cited by 93 publications

(48 citation statements)

References 13 publications

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“…LB medium is known to contain little glucose, which disappears after 2 h of growth of E. coli (1). However, it offers several sugars (next to alcohols and organic acids), i.e., trehalose, arabinose, lactose, and maltose, in significant levels (22). In fact, the level of the major sugar component of LB medium (trehalose) has been reported to be as high as 17% (1,22,40).…”

Section: Resultsmentioning

confidence: 99%

“…However, it offers several sugars (next to alcohols and organic acids), i.e., trehalose, arabinose, lactose, and maltose, in significant levels (22). In fact, the level of the major sugar component of LB medium (trehalose) has been reported to be as high as 17% (1,22,40). Trehalose (with an ␣,␣-1,1-glucoside bond between two ␣-glucose units) may thus offer the major entrance to the glycolytic pathway for organisms growing in LB medium.…”

Section: Resultsmentioning

confidence: 99%

“…It has a rich content of amino acids, peptides, water-soluble vitamins, growth factors, trace elements, and diverse carbohydrates. A major carbohydrate of LB medium is the disaccharide trehalose (2,22).…”

Section: Methodsmentioning

confidence: 99%

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Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Puentes-Téllez

Hansen

Sørensen

et al. 2013

Appl Environ Microbiol

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bIn a study aiming to assess bacterial evolution in complex growth media, we evaluated the long-term adaptive response of Escherichia coli MC1000 in Luria-Bertani (LB) medium. Seven parallel populations were founded and followed over 150 days in sequential batch cultures under three different oxygen conditions (defined environments), and 19 evolved forms were isolated. The emergence of forms with enhanced fitness was evident in competition experiments of all evolved forms versus the ancestral strain. The evolved forms were then subjected to phenotypic and genomic analyses relative to the ancestor. Profound changes were found in their phenotypes as well as whole-genome sequences. Interestingly, considerable heterogeneity was found at the intrapopulational level. However, consistently occurring parallel adaptive responses were found across all populations. The evolved forms all contained a mutation in galR, a repressor of the galactose operon. Concomitantly, the new forms revealed enhanced growth on galactose as well as galactose-containing disaccharides. This response was likely driven by the LB medium.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Puentes-Téllez

Hansen

Sørensen

et al. 2013

Appl Environ Microbiol

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“…Thus, it has been found that carbohydrates such as glucose are vital sources for microbial cell growth and biosurfactant production (Casas et al 1997). Hence, it is important to characterize the key role of the carbon source in the fermentative bioprocess for surfactin production through optimization of fermentation media (Hanko & Rohrer 2000). Therefore, the development of a fermentation process model for up scaling and bioreactor design is necessary.…”

Section: Model Kinetik Pertumbuhan Bakteria Dan Penghasilan Metabolitmentioning

confidence: 99%

Kinetics of Surfactin Production by Bacillus subtilis in a 5 L Stirred-tank Bioreactor

Mubarak

Jufri

Zahar

et al. 2017

JSM

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“…Weather conditions including high temperatures and humidity also have major impact on the cane deterioration. In sugarcane, it has been observed that quality losses in cane is primarily due to chemical (acid) and enzymatic inversion wherein the sucrose could be hydrolyzed to the respective reducing sugars (glucose and fructose) by the acid invertase enzyme (acid inversion of sucrose) which is secreted by few yeast species like Saccharomyces (Hanko and Rohrer 2000). Juice quality losses may also be due to microbial contamination, mainly by the Leuconostoc spp.…”

Section: Losses Due To Delayed Harvestmentioning

confidence: 98%

Feasibility of Sustaining Sugars in Sweet Sorghum Stalks During Post-Harvest Stage by Exploring Cultivars and Chemicals: A Desk Study

et al. 2012

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In the recent years sweet sorghum is emerging as an important feedstock for bioethanol production. It was observed that total soluble sugar yield (TSSY) increases with time in the post-anthesis phase depending on the length of crop cycle. The qualitative and quantitative sugar loss of up to 50% or more occurs due to delay in harvest during post-physiological maturity stage depending on the genotype, weather and soil conditions, and the time lag between harvest and crushing of the stalks. Hence, a desk study was conducted to identify suitable cultivars and/or explore the use of chemicals that sustain sugars in the postharvest phase. In case of delayed harvest beyond physiological maturity stage, growing of cultivars such as SPSSV 30, ICSV 25275, ICSV 25280 and SPV 422 that sustain sugar yield at post-physiological maturity, is recommended. As there are no published reports on sweet sorghum, the literature from sugarcane and wine industries were analyzed and inferences drawn from these industries suggest the evaluation of chemicals like sodium benzoate, potassium metabisulphate, sodium metabisulphite, ammonia, SO 2 , vanillin and acetic acid (vinegar) which may arrest the post-harvest deterioration of sweet sorghum stalks before juice extraction.

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Determination of Carbohydrates, Sugar Alcohols, and Glycols in Cell Cultures and Fermentation Broths Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

Cited by 93 publications

References 13 publications

Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Adaptation and Heterogeneity of Escherichia coli MC1000 Growing in Complex Environments

Kinetics of Surfactin Production by Bacillus subtilis in a 5 L Stirred-tank Bioreactor

Feasibility of Sustaining Sugars in Sweet Sorghum Stalks During Post-Harvest Stage by Exploring Cultivars and Chemicals: A Desk Study

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