Determination of carbon‐14 and/or tritium in the methyl group of organic compounds. Isotopic C‐methyl analysis

Abstract

The determination of the millimolar radioactivity of C‐methyl groups in selected carbon‐14 and tritium labelled compounds by modification of the Kuhn‐Roth method is presented. Oxidation of the radioactive sample in an open vessel using a solution of sodium dichromate and sulfuric acid allows radioassay of the C‐methyl group as acetic acid. This procedure has been tested with a representative group of labelled compounds and found to be isotopically reliable.

Small‐scale degradation of carbon‐14 labelled carboxylic acids. II. Complete degradation of carbon‐14 labelled propionic and n‐butyric acids

“…The degradation procedure consists of a combination of two reactions, the first being the decarboxylation of the silver salt of a carboxylic acid by the modified Hunsdiecker reaction-Method A (13) and the second being the oxidation of C-methyl compounds to acetic acid by the modification of the Kuhn-Roth process-Method B (14). These reactions are illustrated in Figures 1 and 2 and the radiochemical results are given in Tables I and 11.…”