Determination of carcinoembryonic antigen as a tumor marker using a novel graphene-based label-free electrochemical immunosensor

Jozghorbani, Maryam; Fathi, Mojtaba; Kazemi, Sayed Habib; Alinejadian, Navid

doi:10.1016/j.ab.2020.114017

Cited by 79 publications

(33 citation statements)

References 33 publications

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“…The linear detection range of this biosensor was 0.1-5 ng mL −1 , and the LOD was 0.05 ng mL −1 , to detect carcinoembryonic antigen. In addition, the developed sensor was examined in human blood serum for CEA detection, and the results correlated well with those obtained using the standard enzyme-linked immunosorbent assay (ELISA) [122].…”

Section: Graphene-based Nanomaterialsmentioning

confidence: 56%

“…Transferrin-linked polymeric nanocarriers DOX release in the intravacuolar compartments following endocytosis, favoring better targeting efficiency against leukemia [122] Polydopamine-layered zein nanocarriers Increased cellular uptake, ROS production, and induction of apoptosis in the glioma cells, approaching efficient therapy against GBM. [123] Poly-L-lysine-based SEDDS Proficient targeting with eradication of Salmonella typhi and 100% survival.…”

Section: Glutathione-targeted Nanocarriersmentioning

confidence: 99%

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Amino Acids, Peptides, and Proteins: Implications for Nanotechnological Applications in Biosensing and Drug/Gene Delivery

Laraib

Arshad

et al. 2021

Nanomaterials

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Over various scientific fields in biochemistry, amino acids have been highlighted in research works. Protein, peptide- and amino acid-based drug delivery systems have proficiently transformed nanotechnology via immense flexibility in their features for attaching various drug molecules and biodegradable polymers. In this regard, novel nanostructures including carbon nanotubes, electrospun carbon nanofibers, gold nanoislands, and metal-based nanoparticles have been introduced as nanosensors for accurate detection of these organic compounds. These nanostructures can bind the biological receptor to the sensor surface and increase the surface area of the working electrode, significantly enhancing the biosensor performance. Interestingly, protein-based nanocarriers have also emerged as useful drug and gene delivery platforms. This is important since, despite recent advancements, there are still biological barriers and other obstacles limiting gene and drug delivery efficacy. Currently available strategies for gene therapy are not cost-effective, and they do not deliver the genetic cargo effectively to target sites. With rapid advancements in nanotechnology, novel gene delivery systems are introduced as nonviral vectors such as protein, peptide, and amino acid-based nanostructures. These nano-based delivery platforms can be tailored into functional transformation using proteins and peptides ligands based nanocarriers, usually overexpressed in the specified diseases. The purpose of this review is to shed light on traditional and nanotechnology-based methods to detect amino acids, peptides, and proteins. Furthermore, new insights into the potential of amino protein-based nanoassemblies for targeted drug delivery or gene transfer are presented.

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Section: Graphene-based Nanomaterialsmentioning

confidence: 56%

Section: Glutathione-targeted Nanocarriersmentioning

confidence: 99%

Amino Acids, Peptides, and Proteins: Implications for Nanotechnological Applications in Biosensing and Drug/Gene Delivery

Laraib

Arshad

et al. 2021

Nanomaterials

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“…Usually, the CEA content in biological samples is very low, and the threshold value of CEA in human serum is 5.0 ng/ml. When the CEA content in serum is greater than 5.0 ng/ml, it may be a precursor of lung cancer ( Gu et al, 2018 ; Jozghorbani et al, 2021 ). Therefore, the detection of CEA is particularly important.…”

Section: Introductionmentioning

confidence: 99%

Label-free Electrochemical Impedance Spectroscopy Aptasensor for Ultrasensitive Detection of Lung Cancer Biomarker Carcinoembryonic Antigen

et al. 2021

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In this work, an integrated electrode system consisting of a graphene working electrode, a carbon counter electrode and an Ag/AgCl reference electrode was fabricated on an FR-4 glass fiber plate by a polyethylene self-adhesive mask stencil method combined with a manual screen printing technique. The integrated graphene electrode was used as the base electrode, and AuNPs were deposited on the working electrode surface by cyclic voltammetry. Then, the carcinoembryonic antigen aptamer was immobilized using the sulfhydryl self-assembly technique. The sensor uses [Fe(CN)6]3−/4− as a redox probe for label free detection of carcinoembryonic antigen based on the impedance change caused by the difference in electron transfer rate before and after the binding of carcinoembryonic antigen aptamer and the target carcinoembryonic antigen. The results showed a good linear relationship when the CEA concentration is in the range of 0.2–15.0 ng/ml. The detection limit was calculated to be 0.085 ng/ml (S/N = 3).

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“…Graphene is now replacing carbon nanotubes in the development of many sensors. For example, Jozghorbani et al (2021) developed a label-free electrochemical immunosensor for the detection of CEA. They modified reduced graphene oxide (rGO) on the surface of a glassy carbon electrode to form an interface for binding antibodies.…”

Section: Introductionmentioning

confidence: 99%

Carbon Material Based Electrochemical Immunosensor for Gastric Cancer Markers Detection

Zhang

Peng

et al. 2021

Front. Chem.

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Gastric cancer is one of the most common malignant tumors, and early diagnosis will be of great significance to improve the survival quality and overall treatment outcome evaluation of patients. Nanoelectrochemical immunosensor is an emerging biosensor combining nanotechnology, electrochemical analysis method and immunological technology, which has simple operation, fast analysis speed, high sensitivity, and good selectivity. This mini-review summarized immunoassay techniques, nanotechnology and electrochemical sensing for the early detection of gastric cancer. In particular, we focus on the tension of carbon nanomaterials in this field, including the functionalized preparation of materials, signal enhancement and the construction of novel sensing interfaces. Currently, various tumor markers are being developed, but the more recognized gastric cancer tumor markers are carcinoembryonic antigen (CEA), carbohydrate antigen (CA), CD44V9, miRNAs, and programmed death ligand 1. Among them, the electrochemical immunosensor allows the detection of CEA, CA, and miRNAs. The mini-review focused on the development of using carbon based materials, especially carbon nanotubes and graphene for immunosensor fabrication and gastric cancer markers detection.

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Determination of carcinoembryonic antigen as a tumor marker using a novel graphene-based label-free electrochemical immunosensor

Cited by 79 publications

References 33 publications

Amino Acids, Peptides, and Proteins: Implications for Nanotechnological Applications in Biosensing and Drug/Gene Delivery

Amino Acids, Peptides, and Proteins: Implications for Nanotechnological Applications in Biosensing and Drug/Gene Delivery

Label-free Electrochemical Impedance Spectroscopy Aptasensor for Ultrasensitive Detection of Lung Cancer Biomarker Carcinoembryonic Antigen

Carbon Material Based Electrochemical Immunosensor for Gastric Cancer Markers Detection

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