Determination of carnitine and acylcarnitines in biological samples by capillary electrophoresis–mass spectrometry

Heinig, Katja; Henion, Jack D.

doi:10.1016/s0378-4347(99)00417-x

Cited by 82 publications

(71 citation statements)

References 37 publications

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“…This can be nicely seen in the analysis of acylcarnitines with acylchainlength from n = 0 to n = 16 by CE-MS [58]. Separation was possible with aqueous, nonaqueous, and mixed buffers.…”

Section: Quantitative Aspects Of Ms Instrumentationmentioning

confidence: 80%

Quantitation in capillary electrophoresis-mass spectrometry

2005

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Quantitation in capillary electrophoresis-mass spectrometryCE-MS has evolved into a strong alternative to LC-MS. Most of CE-MS applications deal with characterization and identification. However, quantitative aspects have gained importance in, e.g., pharmaceutical and biotechnological applications. Here we summarize and evaluate various methodological aspects in order to achieve sensitive and reproducible results. Similar to LC-MS, aspects of matrix influence on the electrospray process need to be carefully addressed when quantitative results are intended by CE-MS. Due to a more complicated coupling special emphasis needs to be put on the CE-MS interface. Generally linearity over more than three orders of magnitude can be achieved by CE-ESI-MS. Furthermore, a literature survey has been performed in order to give an overview over quantitative measurements performed by CE-MS. The precision can be doubled when changing from a structural related to an isotopically labeled internal standard. Thus a level of precision better than 5% RSD can be achieved.

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“…This can be nicely seen in the analysis of acylcarnitines with acylchainlength from n = 0 to n = 16 by CE-MS [58]. Separation was possible with aqueous, nonaqueous, and mixed buffers.…”

Section: Quantitative Aspects Of Ms Instrumentationmentioning

confidence: 80%

Quantitation in capillary electrophoresis-mass spectrometry

2005

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“…Clearly, for CE-MS, the electrolyte has to be optimized with respect to both separation selectivity and compatibility with the MS interface or process [11,[148][149][150][151].…”

Section: Quantitative Ce-msmentioning

confidence: 99%

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

et al. 2005

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This review is focused on the capillary electrophoresis-mass spectrometric (CE-MS) analysis of nucleic acid constituents in the broadest sense, going from nucleotides and adducted nucleotides over nucleoside analogues to oligonucleotides. These nucleic acid constituents play an important role in a variety of biochemical processes. Hence, their isolation, identification, and quantification will undoubtedly help reveal the process of life and disease mechanisms, such as carcinogenesis, and can also be useful for antitumor and antiviral drug research to provide valuable information about mechanism of action, pharmacokinetics, pharmacodynamics, toxicity, therapeutic drug level monitoring, and quality control related to this substance class. Fundamental investigations into their structure, the search for modifications, the occurrence and biochemical impact of structural variation amongst others, are therefore of great value. In view of the related bioanalytical procedures, the coupling of CE to MS has emerged as a powerful tool for the analysis of the complex mixtures of nucleic acid constituents: CE confers rapid analysis and efficient resolution, while MS provides high selectivity and sensitivity with structural characterization of minute amounts of compound. After an introduction about the biochemical and analytical perspectives on the nucleic acid constituents, the different modes of CE used in this field of research as well as the relevant CE-MS interfaces and the difficulties associated with quantitative CE-MS are briefly discussed. A large section is finally devoted to field-oriented applications.

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“…The separated analytes are commonly detected by an on-column UV-absorbance or laser-induced fluorescence (LIF) detectors. Several protocols have been developed for determination of AAs [32][33][34] and organic acids [35][36][37][38][39][40] such as orotic acid [41][42][43] in urine or blood samples using CE. Direct measurements can often be performed on samples with minimum initial pretreatment -i.e., centrifugation and dilution, if needed.…”

Section: Capillary Electrophoresismentioning

confidence: 99%

“…The increased interest in the analysis of aminothiols in body fluids during the last years resulted in the development of the CE protocol for the simultaneous analysis of homocysteine, cysteine, glutathione, cysteinylglycine, g-glutamylcysteine, and of penicillamine, mercaptopropionylglycine, and cysteamine, three compounds used to treat dis- Due to the lack of a suitable commercial instrumentation the on-line coupling of CE with MS has not yet reached the level of LC-MS; however, the separation efficiency, speed and little or no need for the sample pretreatment make such a combination attractive for a number of applications [33][34][35]49]. Similarly to HPLC, the CE separation serves mainly as a sample desalting and preseparation technique eliminating the interferences of the sample matrix.…”

Section: Capillary Electrophoresismentioning

confidence: 99%

Capillary electrophoresis and mass spectrometry for screening of metabolic disorders in newborns

2004

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Clinical analyses always represent a challenge for the sensitivity and selectivity of the analytical techniques. Of the most critical are the techniques required for the quick determination of the disease state and application of the proper treatment in newborns. This short critical review overviews the present state of the art of the use of mass spectrometry and capillary electrophoresis for screening of metabolic disorders in newborns.

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Determination of carnitine and acylcarnitines in biological samples by capillary electrophoresis–mass spectrometry

Cited by 82 publications

References 37 publications

Quantitation in capillary electrophoresis-mass spectrometry

Quantitation in capillary electrophoresis-mass spectrometry

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

Capillary electrophoresis and mass spectrometry for screening of metabolic disorders in newborns

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