Determination of Cattle Foot-and-Mouth Disease Virus by Micro-ELISA Method

Dong, Yiyang; Xu, Yan; Liu, Zaixin; Fu, Yuanfang; Ohashi, T.; Mawatari, Kazuma; Kitamori, Takehiko

doi:10.2116/analsci.30.359

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…By extension, the use of tandem repeats of 3B peptides to effectively multiply the number of potential antibody binding sites on the antigen has been used successfully to differentiate infected and vaccinated animals (18,48). In addition, others have developed DIVA tests by expressing the entire 3B protein individually or as part of a larger FMDV NSP protein, typically 3AB, 2C3AB, or 3ABC (for recent examples, see references 31,45,[49][50][51]. In each case, however, the sequence used was derived from a single FMDV strain.…”

Section: Discussionmentioning

confidence: 99%

Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B

et al. 2015

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bAn amino acid consensus sequence for the seven serotypes of foot-and-mouth disease virus (FMDV) nonstructural protein 3B, including all three contiguous repeats, and its use in the development of a pan-serotype diagnostic test for all seven FMDV serotypes are described. The amino acid consensus sequence of the 3B protein was determined from a multiple-sequence alignment of 125 sequences of 3B. The consensus 3B (c3B) protein was expressed as a soluble recombinant fusion protein with maltosebinding protein (MBP) using a bacterial expression system and was affinity purified using amylose resin. The MBP-c3B protein was used as the antigen in the development of a competition enzyme-linked immunosorbent assay (cELISA) for detection of anti-3B antibodies in bovine sera. The comparative diagnostic sensitivity and specificity at 47% inhibition were estimated to be 87.22% and 93.15%, respectively. Reactivity of c3B with bovine sera representing the seven FMDV serotypes demonstrated the pan-serotype diagnostic capability of this bioreagent. The consensus antigen and competition ELISA are described here as candidates for a pan-serotype diagnostic test for FMDV infection. Foot-and-mouth disease (FMD) is the most contagious viral vesicular disease that affects cloven-hoofed livestock species. FMD has significant global socioeconomic consequences, from national livestock industries suffering because of international trade restrictions to subsistence farmers suffering because of losses of stock productivity and livelihoods. Western European, North American, and Far East Asian/Pacific regions and most South American countries have official recognition of freedom-from-FMD status, with or without vaccination, by the World Organization for Animal Health (OIE). Regions in which FMD remains endemic tend to be those of lesser economic capacity, and this limits their ability to control or to eradicate the disease (1, 2). As a result, FMD remains an ongoing problem in regions in which it is endemic, and it is a persistent threat to regions that are free of the disease. Seven serotypes have been described for foot-and-mouth disease virus (FMDV), i.e., O, A, C, Asia 1, and South African Territories 1 (SAT1), SAT2, and SAT3. The disease forms produced by each serotype are clinically indistinguishable (3); similarly, FMD is clinically indistinguishable from other vesicular lesion-causing diseases, such as vesicular stomatitis, swine vesicular exanthema, and swine vesicular disease, and laboratory-based clinical diagnosis is required (4, 5). FMD infections may be mild or subclinical in ovine or caprine species, further complicating clinical diagnosis (6, 7). Exposure to one serotype does not confer cross-serotype immunity, potentially complicating diagnosis when multiple serotypes are circulating during an outbreak (8). A definitive diagnosis of FMD is possible only with laboratory testing.Conventional serological assays for FMD, including the virus neutralization test, liquid-phase blocking enzyme-linked immunosorbent assay (ELISA), and so...

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Section: Discussionmentioning

confidence: 99%

Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B

et al. 2015

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“…Conventional ELISA tests for identification of infections are performed on micro-titer plates and it is exhausting assay [43]. A microchip based ELISA (micro ELISA) has also been developed recently by introducing micro beads with immobilized antibodies into a micro channel.…”

Section: Serological Methodsmentioning

confidence: 99%

An Overview of Experimental Rodent Pathogens: Health Monitoring and Latest Diagnostic Techniques

Shaik

Bobby²,

Syed

2022

JASR

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The system of Rodent health monitoring is critically and crucially dependent upon diagnostics. There are two most important reasons to keep research rodents healthy and free from infections those are; to protect the health and welfare of research rodents and detection and subsequent elimination of disease factors at early stages by using ideal methods can prevent infections from negatively impacting research. Sub clinical infections in rodents modify or alteration in research outcome. In India, over and above 1500 facilities are using laboratory animals for biomedical research. However, majority of them have not implemented the comprehensive disease diagnostic programme or health monitoring due to prohibitive cost of the diagnostic kits. Few facilities in India have implemented international guidelines in health monitoring/disease diagnosis and this includes conventional methods, ELISA and PCR for rapid diagnosis. The expansion of various molecular techniques has developed diagnostic procedures by providing specific diagnosis or detailed characterization of any pathogen or host pathogen interactions. Some of the current immunological and molecular techniques in addition to the conventional ones for the diagnostic technique are discussed in brief. These techniques have been advanced and recognized elsewhere needs to be implemented for rapid and accurate diagnosis of rodent pathogens.

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“…This method was demonstrated to be capable of detecting anti-3ABC antibodies in infected swine and cattle sera with good sensitivity and reproducibility. This assay is much faster (within 25 min) and requires lower serum volume (83,84). Apart from the microchip-based ELISA, a chemiluminescence immunoassay (CLIA) was also developed for rapid identification of the anti-NSP antibodies.…”

Section: Differentiation/discrimination Of Infected From Vaccinated Amentioning

confidence: 99%

Advances in the Diagnosis of Foot-and-Mouth Disease

et al. 2020

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Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposition of trade embargo. Rapid and accurate diagnoses are imperative to control this infectious virus. In the current review, enzyme-linked immunosorbent assay (ELISA)-based methods used in FMD diagnosis are extensively reviewed, particularly the sandwich, liquid-phase blocking, and solid-phase competition ELISA. The differentiation of infected animals from vaccinated animals using ELISA-based methods is also highlighted, in which the role of 3ABC polyprotein as a marker is reviewed intensively. Recently, more studies are focusing on the molecular diagnostic methods, which detect the viral nucleic acids based on reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP). These methods are generally more sensitive because of their ability to amplify a minute amount of the viral nucleic acids. In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. Apart from RT-PCR and RT-LAMP, another diagnostic assay specifically designed for on-site diagnosis is the lateral flow immunochromatographic test strips. These test strips have some distinct advantages over other diagnostic methods, whereby the assay often does not require the aid of an external device, which greatly lowers the cost per test. In addition, the on-site diagnostic test can be easily performed by untrained personnel including farmers, and the results can be obtained in a few minutes. Lastly, the use of FMDV diagnostic assays for progressive control of the disease is also discussed critically.

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Determination of Cattle Foot-and-Mouth Disease Virus by Micro-ELISA Method

Cited by 6 publications

References 15 publications

Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B

Pan-Serotype Diagnostic for Foot-and-Mouth Disease Using the Consensus Antigen of Nonstructural Protein 3B

An Overview of Experimental Rodent Pathogens: Health Monitoring and Latest Diagnostic Techniques

Advances in the Diagnosis of Foot-and-Mouth Disease

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