2020
DOI: 10.1002/bio.3971
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Determination of cefixime in pure form, in pharmaceutical products and biological samples through fluorescence quenching of eosin Y

Abstract: A simple, economic and validated spectrofluorimetric method was developed to assay cefixime (CFX). The technique relies on the quenching effect of CFX on the fluorescence intensity of eosin Y in the presence of acetate buffer pH 3.4 to produce an ion‐pair complex that is measured at 549 nm using an excitation wavelength of 300 nm. Reaction‐influencing factors were carefully investigated and optimized. The fluorescence quenching value was linear to the CFX concentration in the range 0.2–40 μg/ml with a correlat… Show more

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Cited by 5 publications
(3 citation statements)
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“…The MFX standard solution (100 μg/ml) was combined with 1.0 ml of plasma or urine. After that, the sample was deproteinized using 6.0 ml of acetonitrile [24][25][26]. The mixture was centrifuged for 15 min at 3000 rpm, and the clear liquid volume was then adjusted with distilled water to 50 ml.…”
Section: Evaluation Of Spiked Biological Samplesmentioning
confidence: 99%
See 1 more Smart Citation
“…The MFX standard solution (100 μg/ml) was combined with 1.0 ml of plasma or urine. After that, the sample was deproteinized using 6.0 ml of acetonitrile [24][25][26]. The mixture was centrifuged for 15 min at 3000 rpm, and the clear liquid volume was then adjusted with distilled water to 50 ml.…”
Section: Evaluation Of Spiked Biological Samplesmentioning
confidence: 99%
“…In our previous projects, eosin and L-tyrosine were used as a fluorescence probe for the determination of drugs [24,25]. However, in this project, we studied the interaction of L-tryp with MFX to develop a simple, cost-effective and interference-free spectrofluorimetric method for determining MFX in pharmaceutical preparations and biological samples.…”
Section: Mfx Exhibits Strong and Improved Antibacterial Action Againstmentioning
confidence: 99%
“…Mechanisms of quenching involve either the interaction of the drug with EY through electron transfer, 26,27 or more commonly through ion-pair complexation between the drug and EY. [28][29][30] The use of EY as a sensing material has been demonstrated for the analysis of many drugs such as antibiotics, 31,32 beta-blockers, 28 and psychoactive drugs. 33 The advantages of using EY for the drug determination include high sensitivity, simplicity, cost effectiveness, and low detection limits.…”
Section: Introductionmentioning
confidence: 99%