Determination of Cefpiramide in Plasma by High-Performance Liquid Chromatographic with Internal Surface Reversed-Phase Silica Column

Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis

“…ISRP supports allowed to withstand several hundred plasma or serum injections (total volume of 6-7 ml) without losing performance [26,27]. GFF material has been recognized as a suitable approach for the direct injection of drugs and metabolites in biological matrices [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43]. This support also shows a real potential for the direct analysis of endogenous substances in serum [44,45] and peptides from complex extracts [46,47].…”

“…In order to evaluate their potential, first commercialized RAM supports (ISRP and SHP) for the direct analysis of compounds in biological fluids, were integrated in direct mode with UV detection [30,34,36,37,40,44,135,141,143]. These extraction supports packed in columns with a conventional dimension (i.e.…”

Determination of warfarin-human serum albumin protein binding parameters by an improved Hummel-Dreyer high-performance liquid chromatographic method using internal surface reversed-phase columns

“…Studies have demonstrated that plasma protein can be recovered from ISRP columns on the order of 99 • 3% at neutral pH (19). The ISRP columns have been used for HPLC assays with direct injection of serum or plasma in a variety of clinical applications (20)(21)(22)(23)(24)(25).…”

Protein binding of cefpiramide in the plasma of various species

“…Adsorption of cefpiramide to the device was negligible. The total and free (ultrafiltrate) concentrations of cefpiramide were measured by HPLC (Ohshima et al 1988). An 1 Correspondence and present address: T. Ohshima, The Research Laboratory for Development of Medicine, School of Pharmacy, Hokuriku University, Kanazawa 920-1 I, Japan.…”