Determination of cefsulodin, cefotiam, cephalexin, cefotaxime, desacetyl-cefotaxime, cefuroxime and cefroxadin in plasma and urine by high-performance liquid chromatography

Lecaillon, J. B.; Rouan, Marie-Claude; Souppart, C.; Febvre, N.; Juge, F.

doi:10.1016/s0378-4347(00)80438-7

Cited by 89 publications

(30 citation statements)

References 8 publications

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“…Levels of cefotaxime and its derivative desacetyl cefotaxime were assayed by high-pressure liquid chromatography as described by Lecaillon et al (15) with minor modifications. The sensitivity of the method was 0.05 ,ug/ml, and the coefficient of variation was 1 to 3%.…”

mentioning

confidence: 99%

Cefotaxime diffusion into cerebrospinal fluid of children with meningitis

Asmar¹,

Thirumoorthi²,

Buckley³

et al. 1985

Antimicrob Agents Chemother

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Cefotaxime diffused consistently and in therapeutic levels into the cerebrospinal fluid (CSF) of 13 children successfully treated for bacterial meningitis. CSF cefotaxime levels early (6.0 ,ug/mI) and late (1.2 ,ug/ml) in treatment were severalfold the MBCs for the infecting organisms. After a single 40-mg/kg dose to each of five infants with ventriculostomies, mean CSF levels of cefotaxime were 6.4, 5.7, and 4.5 ,ug/mI at 2, 4, and 6 h, respectively.

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mentioning

confidence: 99%

Cefotaxime diffusion into cerebrospinal fluid of children with meningitis

Asmar¹,

Thirumoorthi²,

Buckley³

et al. 1985

Antimicrob Agents Chemother

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“…Cefroxadine. Two HPLC methods appeared for quantifying cefroxadine (11,44). Both methods required separation and quantitation using C8 RP columns and phosphoric acidmethanol mobile phases.…”

Section: Methodsmentioning

confidence: 99%

Recent analytical methods for cephalosporins in biological fluids

Toothaker¹,

Wright²,

Pachla³

1987

Antimicrob Agents Chemother

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Since 1980, RP chromatography has been the principal analytical technique used for cephalosporins. This technology offers selectivity, accuracy, and ease of use. Most of the methods rely on protein precipitation and, to a lesser extent, solid-phase isolation or extraction procedures. The proper selection of a method depends on the analytical constraints imposed by the overall objective of the study. For example, pharmacokinetic datum interpretation mandates that the method be validated and provide specific and accurate results. LC is the preferred technique, since it not only meets these specifications but may also distinguish between the drug and metabolites. Those chromatographic methods which quantify several different cephalosporins are not desirable for pharmacokinetic datum interpretation, since accuracy and precision are usually compromised in order that many different drugs may be quantified in a single analysis. The proper selection of sample preparation method is dependent on the presence of potential interferences and the acceptable lower limit of quantitation. Protein precipitation methods offer ease of sample preparation but may suffer from nonselectivity. Solid-phase isolation and extraction procedures may increase selectivity and improve the limit of quantitation. Although LC provides specific and accurate results, clinical laboratories may prefer to use the less specific methods for therapeutic drug monitoring. In this case, microbiological, enzymatic, and fluorimetric methods offer improved sample throughput but less specificity. However, these methods should not be used for drugs that may have a low margin of safety or if the patient is on multiple-antibiotic therapy. Future methods may involve incorporating solid-phase isolation columns to enhance the specificity of chromatographic, microbiological, enzymatic, and fluorescence methods. Advancements in microbore column technology may allow improvements in the selectivity and sensitivity of LC methods. Many investigators prefer to use simple protein precipitation procedures for sample preparation because of sample throughput constraints. However, advances in robotic sample preparation may allow the more cumbersome solid-phase isolation or extraction techniques to be used to improved sample throughput and specificity.

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“…Plasma and urine samples were stored at -20°C until analysis. Preliminary studies have shown that cefotiam is stable in these conditions for several months (Lecaillon et al, 1982).…”

Section: Dosing Schedule and Samplingmentioning

confidence: 99%

“…Plasma and urinary concentrations of cefotiam were determined by reversed-phase liquid chromatography (Lecaillon et al, 1982). The limits of quantitation of the method were 0-4 uxnol/1 of plasma and 10 umol/1 of urine.…”

Section: Assay Proceduresmentioning

confidence: 99%