Determination of cell mass in an insoluble lignocellulosic substrate in submerged fermentation

Schaffeld, G.; Illanes, Andrés

doi:10.1007/bf00141983

Cited by 11 publications

(1 citation statement)

References 4 publications

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“…The term "crude protein" is applied to the total nitrogen multiplied by 6.25. The ammonium nitrogen was evaluated by distillation of samples to which NaOH solution was previously added and ammonium evaluated by volumetry (Schaffeld et al 1982). The microelements solution has the following composition (mg per liter): FeSOn.7H20 5.0, MnSO4.H20 1.6, ZnSO4.7 H20 1.4 and CoC1-6 H20 3.7 (Mandels et al 1969) Crude fat was determined by extraction with petroleum ether (60~176 fraction) in a soxhlet apparatus (AOAC 1970), total carbohydrates with the anthrone method (Herbert et al 1971) and crude fiber by a gravimetric method (Jenkins et al 1981).…”

Section: Methodsmentioning

confidence: 99%

Microbial biomass product from apple pomace in batch and fed batch cultures

Hours

Massucco

Ertola

1985

Appl Microbiol Biotechnol

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Apple pomace, a solid waste from apple processing industries, was used as the raw material for production of a protein enriched product. The experiments were conducted on a small scale in Erlenmeyer flasks or 4-1 fermentors in batch and fed-batch processes using Saccharomycopsis lipolytica and Trichoderma reesei in single and mixed cultures. The results obtained indicate the technical feasibility of the process for obtaining products containing 13% to 15% protein, on dry basis, which is adequate for cattle feeding.

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Section: Methodsmentioning

confidence: 99%

Microbial biomass product from apple pomace in batch and fed batch cultures

Hours

Massucco

Ertola

1985

Appl Microbiol Biotechnol

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Sequential acid and enzymic hydrolysis of sugar beet pulp

Schaffeld

Illanes

Mejías

et al. 1987

J of Chemical Tech & Biotech

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Fermentable sugars from beet pulp were obtained in a two‐stage hydrolytic process. The first stage involved mild acid treatment to hydrolyze the hemicellulose, producing a pentose‐rich syrup and a cellulose‐rich fraction. The second stage was the enzymic saccharification of the cellulose fraction with fungal cellulases to produce a glucose‐rich syrup. The acid hydrolysis stage was evaluated to optimize the type of acid, acid concentration, temperature and reaction time. Solubilization was markedly temperature‐dependent, whereas the specificity of hemicellulose degradation depended on the type of acid utilized. The effects of different reaction times, enzyme‐to‐substrate ratios and particle sizes on the enzymic stage were studied. Both saccharification rate and the extent of final conversion to glucose were markedly affected by the enzyme‐to‐substrate ratio. Sugars recovered from the enzymic degradation of cellulose were partially fermented by Saccharomyces cerevisiae ATCC 4126.

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Challenges in Bioconversion of Cellulosic and Partially Soluble Plant Materials in Submerged Culture

Sukan¹

1988

Developments in Food Microbiology—3

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Determination of cell mass in an insoluble lignocellulosic substrate in submerged fermentation

Cited by 11 publications

References 4 publications

Microbial biomass product from apple pomace in batch and fed batch cultures

Microbial biomass product from apple pomace in batch and fed batch cultures

Sequential acid and enzymic hydrolysis of sugar beet pulp

Challenges in Bioconversion of Cellulosic and Partially Soluble Plant Materials in Submerged Culture

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