Determination of cephalosporins and decomposition products by liquid chromatography with indirect electrochemical detection

Fabre, H.; Kok, Wim Th.

doi:10.1021/ac00153a008

Cited by 20 publications

(5 citation statements)

References 39 publications

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“…Chromatographic conditions. Chromatographic conditions were modified from the previously published procedure of Fabre and Kok (6). Separation was accomplished by using a C18 reverse-phase column (4.1 by 300 mm, 10 p) preceded by a C18 guard column (Alltech Associates, Inc., Deerfield, Ill.).…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of cefotaxime and desacetylcefotaxime in patients with liver disease

Sattler

Nichols

et al. 1991

Antimicrob Agents Chemother

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The dispositions of cefotaxime and its metabolite desacetylcefotaxime were investigated in patients with different forms of chronic parenchymal liver disease (CPLD). A total of 31 subjects (27 patients and 4 controls) received a single 2-g dose of cefotaxime by infusion, and serial blood samples were drawn. The area under the concentration-time curve ranged from 176 to 241 micrograms.h/ml, the apparent half-life ranged from 1.49 to 2.42 h, and clearance ranged from 2.06 to 3.10 ml/min/kg in patients with four different forms of CPLD. The area under the concentration-time curve and the apparent half-life of desacetylcefotaxime ranged from 72 to 128 micrograms.h/ml and 7.1 to 13.4 h, respectively. Pharmacokinetic parameters were significantly different in patients with CPLD compared with those in control subjects and were related to clinical indices of hepatic impairment. Modest accumulation of cefotaxime in patients with severe hepatic impairment is unlikely to produce toxicity because of its high therapeutic index, and dosing modifications may not be required.

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Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of cefotaxime and desacetylcefotaxime in patients with liver disease

Sattler

Nichols

et al. 1991

Antimicrob Agents Chemother

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“…A number of analytical methods have been reported for quantification of CF [1] , [2] , [3] , [4] , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] and CA [18] , [19] , [20] , [21] , [22] individually or with other drugs in biological samples. To the best of our information, only one method has been reported for the simultaneous determination of CF and CA [23] .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous pharmacokinetic assessment of cefadroxil and clavulanic acid in human plasma by LC–MS and its application to bioequivalence studies

Nagarajan¹,

Vimal²,

George³

et al. 2013

Journal of Pharmaceutical Analysis

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A simple, rapid and selective liquid chromatography–atmospheric pressure chemical ionization–mass spectrometry (LC–APCI–MS) assay method has been developed and fully validated for the simultaneous quantification of cefadroxil (CF) and clavulanic acid (CA) in human plasma. Analytes and internal standard (IS) were extracted from human plasma by solid-phase extraction (SPE) technique using Sam prep (3 mL, 100 mg) extraction cartridge. The extracted samples were chromatographed on a reverse phase C18 column using a mixture of methanol: acetonitrile: 2 mM ammonium acetate (pH 3.5) (25:25:50, v/v/v) as the mobile phase at a flow rate of 0.8 mL/min. Quantification of the analytes and IS were carried out using single quadrupole LC–APCI–MS through selected-ion monitoring (SIM) at m/z 362 and m/z 198, for CF and CA, respectively. Method validation was performed as per the FDA guidelines and the results met the acceptance criteria. Plasma concentration of CF and CA followed by the oral administration of CF/CA (500/125 mg) pill to healthy male volunteers (n=12) was measured. Area under plasma concentration–time curve from 0 to 12 h (AUC0–12 h) and 0 h extrapolated to infinity (AUC0−∞) were calculated. The ratio of AUC0–12 h/AUC0−∞ was found to be >85% for all the subjects, as recommended by the FDA guidelines.

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“…It is well known that cephalosporins, including cephalothin, are not stable under either alkaline or acidic conditions (25,(37)(38)(39)(40)(41). It appears that the base-acid-base treatment of cephalothin in the present study caused destruction of the cephalothin molecule.…”

Section: Discussionmentioning

confidence: 54%

A Polarographic Study of the Reactivity of Cephalothin with Alkaline Picrate

Shi

Blass

1996

Microchemical Journal

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Determination of cephalosporins and decomposition products by liquid chromatography with indirect electrochemical detection

Cited by 20 publications

References 39 publications

Pharmacokinetics of cefotaxime and desacetylcefotaxime in patients with liver disease

Pharmacokinetics of cefotaxime and desacetylcefotaxime in patients with liver disease

Simultaneous pharmacokinetic assessment of cefadroxil and clavulanic acid in human plasma by LC–MS and its application to bioequivalence studies

A Polarographic Study of the Reactivity of Cephalothin with Alkaline Picrate

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