Determination of cephalosporins in raw bovine milk by high-performance liquid chromatography

Sørensen, Lambert K.; Snor, Lena

doi:10.1016/s0021-9673(99)01317-5

Cited by 83 publications

(55 citation statements)

References 6 publications

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“…However, a number of methods for the determination of cefquinome were reported for milk and animal tissues. These methods include high-performance liquid chromatography with UV detector (HPLC-UV) (17,18,21), HPLC with diode array detector (5), liquid chromatography combined with tandem mass spectrometry (1), and screening methods (7,8,13,14,18). To the authors' knowledge, only two published papers involving analytical methods for the determination of cefquinome in plasma, one employing liquid chromatography combined with electrospray tandem mass spectrometry (LC-ESI-MS-MS) (11) and one using HPLC-UV (9), reported complete validation procedures.…”

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confidence: 99%

Development and Validation of a High-Performance Liquid Chromatography Method for Determination of Cefquinome Concentrations in Sheep Plasma and Its Application to Pharmacokinetic Studies

Üney

Altan

Elmas

2011

Antimicrob Agents Chemother

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Cefquinome has a broad spectrum of antibacterial activity and was developed especially for use in animals. A simple and sensitive high-performance liquid chromatography (HPLC) method with UV-visible detection for quantification of cefquinome concentrations in sheep plasma was developed and validated. Separation of cefquinome from plasma components was achieved on a Phenomenex Gemini C 18 column (250 mm by 4.6 mm; internal diameter [i.d.], 5 m). The mobile phase consisted of acetonitrile and 0.1% trifluoroacetic acid in water and was delivered at a rate of 0.9 ml/min. A simple and rapid sample preparation involved the addition of methanol to 200 l of plasma to precipitate plasma proteins followed by direct injection of 50 l of supernatant into the high-performance liquid chromatography system. The linearity range of the proposed method was 0.02 to 12 g/ml. The intraday and interday coefficients of variation obtained from cefquinome were less than 5%, and biases ranged from ؊3.76% to 1.24%. Mean recovery based on low-, medium-, and high-quality control standards ranged between 92.0 and 93.9%. Plasma samples were found to be stable in various storage conditions (freeze-thaw, postpreparative, short-term, and long-term stability). The method described was found to be readily available, practicable, cheap, rapid, sensitive, precise, and accurate. It was successfully applied to the study of the pharmacokinetics of cefquinome in sheep. This method can be very useful and an alternate to performing pharmacokinetic studies in the determination of cefquinome for clinical use.

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confidence: 99%

Development and Validation of a High-Performance Liquid Chromatography Method for Determination of Cefquinome Concentrations in Sheep Plasma and Its Application to Pharmacokinetic Studies

Üney

Altan

Elmas

2011

Antimicrob Agents Chemother

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“…Determination of CEZ concentration in the manure and seed sludge sample was carried out with HPLC [12] after solid phase extraction (SPE) of a sample using a modified method described by Sørensen et al [13]. A diluted sample was centrifuged and supernatant was loaded to a tC18 SPE cartridge (Sep-Pak tC18, Waters, Milford, MA, USA).…”

Section: Analytical Methods Of Cezmentioning

confidence: 99%

Effect of Seed Sludge on Survival of Antibiotic-Resistant Bacteria and Fate of Bacteria Resistant to Different Classes of Antibiotics during Anaerobic Digestion of Dairy Manure

Takemura

Ihara

Kitazono

et al. 2016

J. of Wat. & Envir. Tech.

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Livestock manure is one of main pollution reservoirs of antibiotic-resistant bacteria (ARBs) due to excessive use of veterinary antibiotics. For preventing the environmental spread, an efficient process is required to reduce ARBs in livestock manure. In this work, we investigated the survival of ARB during mesophilic anaerobic digestion of dairy cattle manure. Effects of seed sludge and the antibiotic class were investigated for reduction of ARBs by batch digestion tests. The numbers of all ARBs were 6.34×10 4 to 2.08×10 6 before anaerobic digestion. The numbers of oxytetracycline, chlortetracycline, gentamicin and kanamycin -resistant bacteria decreased close to the detection limit after 20-day anaerobic digestion. However, reduction effects of cefazolin and streptomycin -resistant bacteria were small. The seed sludge had little influence on the survival of ARBs during the anaerobic digestion. The results showed that the ARB reduction effect was influenced by the types of antibiotics, and mostly not by seed sludge.

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“…Octadecyl (C18)-bonded silica is the most widely employed SPE sorbent [37][38][39][40], although other solid phase materials such as LiChrolut EN [41], Isolut ENV1 [42,43] and even weak cation- [3], and strong anionexchangers [44] have also been reported in the literature for this purpose. The elution of the antibiotics retained by these sorbents is usually performed with aqueous methanol or ACN in some cases modified with a buffer.…”

Section: Factors Affecting Spe Preconcentration Of â-Lactam Antibioticsmentioning

confidence: 99%

Use of SDS micelles for improving sensitivity, resolution, and speed in the analysis of β‐lactam antibiotics in environmental waters by SPE and CE

Serrano

Silva

2007

Electrophoresis

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This study dealt with the potential of MEKC with LIF detection involving derivatization with sulfoindocyanine succinimidyl ester (Cy5) for the separation and determination of beta-lactam antibiotics (ampicillin, amoxicillin, cephradine, and cephalexin) in environmental water samples. Water samples of 50 mL were enriched by SPE by passage through a weak base-cation Amberlite(R) IRA-93 exchange column. SDS micelles play important roles in the whole analytical process by improving the yield (sensitivity) and the kinetics of the labeling reaction, the elution of the retained antibiotics from the SPE preconcentration system and the electrophoretic resolution of their Cy5-derivatives. The optimum procedure includes a derivatization step of the antibiotics at 25 degrees C for 10 min and direct injection for MEKC analysis, which is conducted within about 15 min using 15 mM SDS in the running buffer (35 mM sodium borate at pH 9.3). LODs from 30 to 45 ng/L and RSDs (within-day precision) from 3.5 to 5.9% were obtained for the antibiotics in water samples with average recoveries ranging from 96.4 to 99.4%. These results indicate that the method proposed is a straightforward and sensitive tool for the determination of these antibiotics in environmental water samples providing similar quantitative results to those using more expensive equipment like LC-electrospray MS/MS.

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Determination of cephalosporins in raw bovine milk by high-performance liquid chromatography

Cited by 83 publications

References 6 publications

Development and Validation of a High-Performance Liquid Chromatography Method for Determination of Cefquinome Concentrations in Sheep Plasma and Its Application to Pharmacokinetic Studies

Development and Validation of a High-Performance Liquid Chromatography Method for Determination of Cefquinome Concentrations in Sheep Plasma and Its Application to Pharmacokinetic Studies

Effect of Seed Sludge on Survival of Antibiotic-Resistant Bacteria and Fate of Bacteria Resistant to Different Classes of Antibiotics during Anaerobic Digestion of Dairy Manure

Use of SDS micelles for improving sensitivity, resolution, and speed in the analysis of β‐lactam antibiotics in environmental waters by SPE and CE

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