Determination of Chlorogenic Acid, Baicalin and Forsythin in Shuanghuanglian Preparations by HPLC-Dad

Li, Yang; Yan, Qinghua; Mo, Haizhen; Li, Xiaomin; Wang, Qing

doi:10.4067/s0717-97072012000400006

Cited by 8 publications

(5 citation statements)

References 3 publications

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“…In the MS spectra, peak 1 corresponded to a pseudomolecular ion [M-H] − at m/z 353 and a fragment ion at m/z 191 formed from deprotonated quinic acid. This peak was identified as chlorogenic acid (Chen et al 2014;Yang et al 2012). Peak 2 indicated a pseudomolecular ion [M-H] − atm/z 179, identified as caffeic acid (Chen et al 2014;Bravo et al 2007).…”

Section: Identification Of Phenolic Compounds Of the Hydromethanolic mentioning

confidence: 99%

Effect of cytokinins on shoots proliferation and rosmarinic and salvianolic acid B production in shoot culture of Dracocephalum forrestii W. W. Smith

et al. 2018

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The current study estimates the effect of different cytokinins on shoot proliferation and biosynthesis of caffeic acid derivatives in Dracocephalum forrestii in vitro culture. The shoots were grown on Murashige and Skoog (MS) agar medium with 1 µM indole-3-acetic acid (IAA) and different content of 6-benzyloaminopurine (BAP), zeatin, kinetin (1, 2, 4, 8, 18 µM) or thidiazuron (TDZ) (0.1, 0.2, 0.5, 1, 2 µM). The highest multiplication rate (about seven shoots and/or buds per explant) was obtained after 4 weeks of culture on MS medium with 1 µM IAA and 8 or 16 µM BAP. Optimal biomass of plant material was also received on the same media. The identity of the compounds present in the hydromethanolic extracts from D. forrestii shoots grown on cytokinin-supplemented media was confirmed using UPLC-PDA-ESI-MS method. The analysis revealed the presence of nine metabolites recognized as caffeic acid derivatives. The content of the predominant phenolic acids in the extracts, i.e. rosmarinic acid (RA) and salvianolic acid B (SAB), was determined with UHPLC. The highest yield of RA was found in shoots cultivated in the medium containing 1 µM IAA and 2 µM BAP (18.7 mg/g DW). The highest level of SAB (5.3-5.9 mg/g DW) was identified in multiple shoots grown in the presence of 1 µM IAA and 0.5-1 µM TDZ or 2 µM BAP.

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Section: Identification Of Phenolic Compounds Of the Hydromethanolic mentioning

confidence: 99%

Effect of cytokinins on shoots proliferation and rosmarinic and salvianolic acid B production in shoot culture of Dracocephalum forrestii W. W. Smith

et al. 2018

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“…Various methods for the determination of CGA have been developed, namely, near-infrared spectroscopy [ 6 – 8 ], capillary electrophoresis [ 9 , 10 ], nano-liquid chromatography-electrospray ionization mass spectrometry [ 11 ], high-performance liquid chromatography [ 12 – 18 ], ultra-performance liquid chromatography [ 19 ], liquid chromatography-mass spectrometry [ 20 , 21 ], chemiluminescence [ 22 , 23 ], and electrochemical methods [ 24 – 27 ]. Among these reported methods, near-infrared spectroscopy requires expensive equipment and resource-intensive calibrations.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric determination of chlorogenic acid in pharmaceutical products using poly(aminosulfonic acid) modified glassy carbon electrode

Chao

2014

Journal of Food and Drug Analysis

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In this work, a poly(aminosulfonic acid) modified glassy carbon electrode was fabricated and the electrochemical behavior of chlorogenic acid (CGA) was studied by cyclic voltammetry. Compared with a bare glassy carbon electrode, the modified electrode exhibits excellent catalytic effect on the electrochemical redox of CGA. Utilizing this catalytic effect, a sensitive and selective electrochemical method for the determination of CGA was developed. The analytical parameters were optimized. Under the optimized conditions, the oxidation peak current is linearly proportional to the concentration of CGA in the range from 4.00 × 10 to 1.20 × 10 mol/L and the detection limit is 4.00 × 10 mol/L. Further, the performance of the proposed method has been validated in terms of linearity (r = 0.9995), recovery (96.3-102.8%), reproducibility (RSD ＜ 4.0%, n = 6) and robustness. The developed method has been successfully applied for the determination of CGA in a variety of pharmaceutical products.

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“…Although quantitative analysis of single and a few compounds in this formula has been reported by capillary electrophoresis with electrochemical detection (CE-ECD) [ 5 ], high performance liquid chromatography with diode array detection (HPLC-DAD) [ 6 ], HPLC with mass spectrometry (HPLC-MS) [ 7 ], HPLC with evaporative light scattering detection (HPLC-ELSD) [ 8 ], and HPLC-DAD-ECD [ 9 ], a simultaneous analysis of flavonoids, lignans and phenylpropanoids in this prescription is still missing through UPLC-ESI-MS/MS based on multiple reaction monitoring (MRM) for speedy quality control.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Multiple Classes of Hydrophilic and Lipophilic Components in Shuang-Huang-Lian Oral Liquid Formulations by UPLC-Triple Quadrupole Linear Ion Trap Mass Spectrometry

2017

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The Shuang-Huang-Lian (SHL) oral liquid is a combined herbal prescription used in the treatment of acute upper respiratory tract infection, acute bronchitis and pneumonia. Multiple constituents are considered to be responsible for the therapeutic effects of SHL. However, the quantitation of the multi-components from multiple classes is still unsatisfactory because of the high complexity of constituents in SHL. In this study, an accurate, rapid, and specific UPLC-MS/MS method was established for simultaneous quantification of 18 compounds from multiple classes in SHL oral liquid formulations. Chromatographic separation was performed on a HSS T3 (1.8 μm, 2.1 mm × 100 mm) column, using a gradient mobile phase system of 0.1% formic acid in acetonitrile and 0.1% formic acid in water at a flow rate of 0.2 mL·min−1; the run time was 23 min. The MS was operated in negative electrospray ionization (ESI−) for analysis of 18 compounds using multiple reaction monitoring (MRM) mode. UPLC-ESI−-MRM-MS/MS method showed good linear relationships (R2 > 0.999), repeatability (RSD < 3%), precisions (RSD < 3%) and recovery (84.03–101.62%). The validated method was successfully used to determine multiple classes of hydrophilic and lipophilic components in the SHL oral liquids. Finally, principal component analysis (PCA) was used to classify and differentiate SHL oral liquid samples attributed to different manufacturers of China. The proposed UPLC-ESI−-MRM-MS/MS coupled with PCA has been elucidated to be a simple and reliable method for quality evaluation of SHL oral liquids.

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Determination of Chlorogenic Acid, Baicalin and Forsythin in Shuanghuanglian Preparations by HPLC-Dad

Cited by 8 publications

References 3 publications

Effect of cytokinins on shoots proliferation and rosmarinic and salvianolic acid B production in shoot culture of Dracocephalum forrestii W. W. Smith

Effect of cytokinins on shoots proliferation and rosmarinic and salvianolic acid B production in shoot culture of Dracocephalum forrestii W. W. Smith

Voltammetric determination of chlorogenic acid in pharmaceutical products using poly(aminosulfonic acid) modified glassy carbon electrode

Simultaneous Determination of Multiple Classes of Hydrophilic and Lipophilic Components in Shuang-Huang-Lian Oral Liquid Formulations by UPLC-Triple Quadrupole Linear Ion Trap Mass Spectrometry

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