Determination of chlorothalonil in water and agricultural products by a magnetic particle-based enzyme immunoassay

Lawruk, Timothy S.; Gueco, Adrian M.; Jourdan, Scott W.; Scutellaro, Adele M.; Fleeker, James R.; Herzog, David P.; Rubio, Fernando

doi:10.1021/jf00053a052

Cited by 32 publications

(14 citation statements)

References 19 publications

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“…Immunoassay-based techniques such as enzyme-linked immunosorbent assay (ELISA), can be simpler than conventional chromatographic analysis and have been used to detect 2,4,5-TP in primary matrixes. A commercial magnetic particle-based kit for detecting residues of 2,4,5-TP is available from Strategic Diagnostics; the lowest detectable quantities are 1.4 µg L -1 and 20 µg L -1 in water and soil, respectively [15].…”

Section: Introductionmentioning

confidence: 99%

Assessment of enzyme-linked immunosorbent assay for the determination of 2,4,5-TP in water and soil

et al. 2002

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A highly sensitive and specific indirect enzyme-linked immunosorbent assay is described for Silvex, 2-(2,4,5 trichlorophenoxy)propionic acid, (2,4,5-TP). One specific feature of the immunoassay is the use of simple chemical activation of chlorophenoxy acids to prepare both the immunizing and coating conjugates. The assay is based on the use of polyclonal antibodies raised against 2,4,5-TP, and a peroxidase-labeled secondary antibody for colorimetric detection. The effect of different chemical conditions (pH, and salt and detergent concentration) on immunoassay performance has been studied. Under the best conditions the least detectable dose and the sensitivity (IC(50)) for 2,4,5-TP were 0.05 micro g L(-1) and 0.80 micro g L(-1), respectively. The optimized immunoassay was also highly specific, showing little (6.9% for 2,4,5-T) or no cross-reactivity with other similar herbicides. The assay was used to determine 2,4,5-TP in water and soils. The excellent recoveries obtained (mean values ranging between 89% and 104%) make this immunoassay a suitable screening method for either environmental monitoring or laboratory quantification of 2,4,5-TP.

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Section: Introductionmentioning

confidence: 99%

Assessment of enzyme-linked immunosorbent assay for the determination of 2,4,5-TP in water and soil

et al. 2002

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“…Lawruk et al also reported that their polyclonal antibody reacted with quintozene and chlorothalonil but not pentachlorophenol 15) . This might be due to the presence of the acidic hydroxyl group in pentachlorophenol.…”

Section: Cross-reactivity Of Prepared Moabsmentioning

confidence: 93%

“…A magnetic particle-based ELISA was developed for chlorothalonil analysis in water and vegetable samples 15) . However, the method used polyclonal antibody (PoAb) which may show variable reactivity.…”

Section: )-8)mentioning

confidence: 99%

野菜に残留する殺菌剤クロロタロニルを測定するための直接競合elisaの開発

Okazaki

Hirakawa²,

Yamaguchi-Murakami³

et al. 2014

Journal of the Food Hygienics Society of Japan

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A direct competitive (dc)-ELISA was developed for rapid and simple determination of chlorothalonil residue in vegetables. A carboxylic acid derivative of pentachlorophenol was used to prepare an anti-chlorothalonil monoclonal antibody (MoAb) that showed adequate reactivity for dc-ELISA. Before homogenization of vegetable samples, phosphoric acid was added (vegetable-10 phosphoric acid (2 : 1, w/v)) to block enzymatic decomposition of chlorothalonil. The use of phosphate buffer (100 mmol/L, pH 7.0) minimized the influence of phosphoric acid on competitive reaction in the dc-ELISA. Working range was 0.10 to 6.0 ng/mL in the optimized dc-ELISA. The recovery of chlorothalonil spiked in cucumber and eggplant was 97.1 to 125 . The results correlated well with those obtained by HPLC analysis. The dc-ELISA could rapidly determine chlorothalonil after a simple sample preparation procedure.

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“…Good correlations have been found using ELISA for the determination of chlorothanil as compared with GC-FID methods [34], and for carbendazim (or benomyl after tra n s fo rm ation into carbendazim) as compare d with LC determinations [35]. Oubina et al [25] evaluated an ELISA for the determination of chlorpyrifos-ethyl and compared the results given by IA with those obtained by automated on-line solid-phase extraction followed by LC-DAD in spiked estuarine waters in the range 0 -4 µg/L.…”

Section: Application To Environmental Monitoring: Data Interpretationmentioning

confidence: 97%

Applications and validation of immunoassays for pesticides analysis

Hennion¹

1998

Analusis

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T he time and expense involved in classical analytical methods (i.e., sampling, sample preparation, and laboratory analysis) limit the number of samples that can be analyzed in environmental surveys. There is a real need for developing fast, easy to use, robust, sensitive, cost-effect ive and fi e l d -a n a lytical techniques. Immu n o a s s ays (IAs) meet these requirements and many pesticides can be analyzed and monitored at regulatory levels without any sample preparation method. They are increasingly used and a few IAs have been recently accepted as screening methods by the US EPA and one for the total determination of triazines.Current research includes the development of new assay form at s , mu l t i -residue assay s , and fl ow-injection immu n o a ss ays. An eva l u ation of the perfo rmances of commerc i a l ly available IAs for pesticides analysis as well as a selection of recent applications in monitoring programs are presented here.I m mu n o a s s ay techniques provide a simple, powerful and inexpensive method for pesticide analysis which has been widely used in monitoring programs especially in the US. Their rapid and recent development witnesses of their increasing acceptance and is the result from the demonstration of quality and validity compared to more traditional techniques.

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Determination of chlorothalonil in water and agricultural products by a magnetic particle-based enzyme immunoassay

Cited by 32 publications

References 19 publications

Assessment of enzyme-linked immunosorbent assay for the determination of 2,4,5-TP in water and soil

Assessment of enzyme-linked immunosorbent assay for the determination of 2,4,5-TP in water and soil

野菜に残留する殺菌剤クロロタロニルを測定するための直接競合elisaの開発

Applications and validation of immunoassays for pesticides analysis

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