Determination of cholesterol using o-phthalaldehyde

Rudel, L L; Morris, Manford D.

doi:10.1016/s0022-2275(20)36896-6

Cited by 979 publications

(69 citation statements)

References 3 publications

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“…The potential of the LAB strains to assimilate cholesterol in MRS broth with and without bile salts was measured using published methods 27 , 43 . Briefly, LAB strains were incubated at 37 °C for 20 h in MRS broth supplemented with sodium tauroglycocholate (6 mM) and cholesterol (70 μg/ml).…”

Section: Methodsmentioning

confidence: 99%

Rhizospheric Lactobacillus plantarum (Lactiplantibacillus plantarum) strains exhibit bile salt hydrolysis, hypocholestrolemic and probiotic capabilities in vitro

Singhal

Singh

Mohanty

et al. 2021

Sci Rep

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Lactobacillus plantarum (renamed as Lactiplantibacillus plantarum) has been isolated from many sources but very rarely from rhizospheric soil. This is the first report on isolation and assessment of probiotic capabilities of L. plantarum strains isolated from rhizospheric soil. The isolates were confirmed by 16S rRNA gene sequencing and named as NS14, NS16 and NGG. All the isolates were evaluated for bile salt hydrolysis, hypocholestrolemic potential and probiotic attributes. Our results indicated that all the strains harboured bsh and showed in vitro cholesterol assimilation capabilities which increased when bile salts were also present in the culture medium. Also, all the strains remained viable at high temperatures and in the presence of NaCl, lysozyme, simulated gastric juice, bile salts and, exhibited auto- and co-aggregation capabilities. Additionally, L. plantarum strain NS14 survived in the presence of phenols, acidic environment (pH 2–3) and was resistant to many clinically relevant antibiotics. Since, L. plantarum NS14 exhibited most of the desirable and essential characteristics of a probiotic it should be further investigated as a potent probiotic with an additional benefit as a hypocholesterolemic biotherapeutic. Moreover, rhizosphere can be explored as a useful ecological niche for isolating microorganisms with biotechnological and probiotic potential.

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Section: Methodsmentioning

confidence: 99%

Rhizospheric Lactobacillus plantarum (Lactiplantibacillus plantarum) strains exhibit bile salt hydrolysis, hypocholestrolemic and probiotic capabilities in vitro

Singhal

Singh

Mohanty

et al. 2021

Sci Rep

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“…Total lipids were extracted from liver, spleen, and small intestine by the Folch method (Folch et al 1957;Du et al 1998a). TG and cholesterol amounts were measured by chemical analysis (Rudel and Morris 1973;Du et al 2001a).…”

Section: Tissue Lipid Analysesmentioning

confidence: 99%

The Role of Mannosylated Enzyme and the Mannose Receptor in Enzyme Replacement Therapy

Levine²,

Ganesa³

et al. 2005

The American Journal of Human Genetics

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Lysosomal acid lipase (LAL) is the critical enzyme for the hydrolysis of triglycerides (TGs) and cholesteryl esters (CEs) in lysosomes. LAL defects cause Wolman disease (WD) and CE storage disease (CESD). An LAL null (lal-/-) mouse model closely mimics human WD/CESD, with hepatocellular, Kupffer cell and other macrophage, and adrenal cortical storage of CEs and TGs. The effect on the cellular targeting of high-mannose and complex oligosaccharide-type oligosaccharide chains was tested with human LAL expressed in Pichia pastoris (phLAL) and CHO cells (chLAL), respectively. Only chLAL was internalized by cultured fibroblasts, whereas both chLAL and phLAL were taken up by macrophage mannose receptor (MMR)-positive J774E cells. After intraperitoneal injection into lal-/- mice, phLAL and chLAL distributed to macrophages and macrophage-derived cells of various organs. chLAL was also detected in hepatocytes. Ten injections of either enzyme over 30 d into 2- and 2.5-mo-old lal-/- mice produced normalization of hepatic color, decreased liver weight (50%-58%), and diminished hepatic cholesterol and TG storage. Lipid accumulations in macrophages were diminished with either enzyme. Only chLAL cleared lipids in hepatocytes. Mice double homozygous for the LAL and MMR deficiences (lal-/-;MMR-/-) showed phLAL uptake into Kupffer cells and hepatocytes, reversal of macrophage histopathology and lipid storage in all tissues, and clearance of hepatocytes. These results implicate MMR-independent and mannose 6-phosphate receptor-independent pathways in phLAL uptake and delivery to lysosomes in vivo. In addition, these studies show specific cellular targeting and physiologic effects of differentially oligosaccharide-modified human LALs mediated by MMR and that lysosomal targeting of mannose-terminated glycoproteins occurs and storage can be eliminated effectively without MMR.

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“…The meat cholesterol was determined using the method [13]. About one gram crushed sample was homogenized with 2 mL 50% (w/v) potassium hydroxide and 3 mL 95% ethanol and incubated at 60ºC in a water bath for 10 min and followed by cooling at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Effect of the Dietary Fat Sources on Performance, Liver Fatty Acid Composition and Meat Cholesterol Content in Broiler

Khatun¹,

Loh²,

Akit³

et al. 2018

IJET

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A study was conducted to evaluate the effect of feeding diet containing palm oil (PO) and sunflower oil (SO) and their combination on bird performance, liver fatty acid profile and meat cholesterol content of broiler. A total of 144 day-old broiler chicks (Cobb) randomly assigned into four dietary treatments and fed for six weeks. The dietary treatments were T1, 6% PO (control); T2, 4% PO + 2% SO; T3, 2% PO + 4% SO; and T4, 6% SO. The body weight gain increased and feed conversion ratio (FCR) reduced with the increasing level of unsaturated fatty acid sources oil (SO) compared to control group (PO). Significantly higher (P<0.05) overall daily body weight gain and lower FCR were found in broilers fed dietary treatments T3 compared to other treatments. However, no differences (P<0.05) were found in daily body weight gain and FCR among the two combination of oil and SO alone. Dietary treatments influence the fatty acid composition of liver and increasing the dietary SO decreased the level of C16:0, C18:0, C18:1n-9, SFA and MUFA and increased the C18:2n-6, UFA: PUFA, UFA: SFA and PUFA: SFA. Birds fed PO had higher cholesterol concentration in meat which was decreased by supplementation of SO in broiler diet. However, cholesterol concentration was not differed in meat among the dietary SO and combination of SO and PO. It could be concluded that supplementation of a combination of PO and SO in broiler diet increased performance, altered the fatty acid composition of liver tissue and decreased cholesterol content of meat which may have a favorable impact on consumer’s health.

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Determination of cholesterol using o-phthalaldehyde

Cited by 979 publications

References 3 publications

Rhizospheric Lactobacillus plantarum (Lactiplantibacillus plantarum) strains exhibit bile salt hydrolysis, hypocholestrolemic and probiotic capabilities in vitro

Rhizospheric Lactobacillus plantarum (Lactiplantibacillus plantarum) strains exhibit bile salt hydrolysis, hypocholestrolemic and probiotic capabilities in vitro

The Role of Mannosylated Enzyme and the Mannose Receptor in Enzyme Replacement Therapy

Effect of the Dietary Fat Sources on Performance, Liver Fatty Acid Composition and Meat Cholesterol Content in Broiler

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