Determination of Chromium(III) Picolinate Using High Performance Liquid Chromatography-Ultraviolet Spectrophotometry

Kim, Sung Il; Woo, Dong Jin; Lee, Myung Hee; Woo, Gun Jo; Kang, Dae‐Kyung; Won, Ki

doi:10.5012/bkcs.2003.24.10.1517

Cited by 8 publications

(1 citation statement)

References 9 publications

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“…Cr-Pic is o cial in the USP as Chromium Tripicolinate (C18H12N3O6Cr) in the dietary supplement section. Few published methods are available for its determination including; ow injection -electrospray ionization -tandem mass spectrometry [13],HPLC [14,15], HPLC-ICP-QQQ-MS[16], Spectrometry using electrothermal atomic absorption in combination with a ow injection system [17], capillary electrophoresis-inductively coupled plasma mass spectrometry [18].The approach was suitable for both chemical and biological applications due to its extraordinarily high sensitivity and consequently low quanti cation limits to HPLC methods. [19] Additionally, it requires considerably less time than other analytical methods.…”

Section: Introductionmentioning

confidence: 99%

A Novel Cyclic Voltamnetric Study for the Selective and Sensitive Electroanalytical Determination of Chromium Picolinate in Absence and Presence of Some Reagents in KBr Medium Using Gold Electrode. Application to Commercial Capsules

Salem,

Gomaa,

Sheribah

et al. 2024

Preprint

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This study aims to investigate the electrochemical behavior of chromium picolonate (Cr-Pic) in a KBr medium using cyclic voltammetry (CV) with an Au electrode. The determination of Cr-Pic is of great importance due to its potential health benefits and industrial applications. The impact of the presence of various reagents on the electrochemical response of Cr-Pic is also discussed. The study further explores the applicability of the developed method for the analysis of Cr-Pic in commercial capsules. Chromium ions gave a reduction wave above 2.0 V, mainly because of the evolution of gases in the medium. In this work, the catalytic chromium peaks have appeared, affected by the gold electrode. The results were analyzed in the presence or absence of the dye Diamond Fuchsial Basic Violet (1.0 10-2) M in order to assess how the dye and metal ions will interact in solution. We assess the values of the solvation and kinetic parameters (surface coverage, heterogeneous rate constant, diffusion coefficient, and amount of electricity) based on this research. Moreover, the impact of various reactant concentrations, scan rates, and temperatures Different concentrations of Cr-Pic were applied, and the redox peaks were analyzed. Oxidation and reduction waves of Cr-Pic were linearly dependent on its concentration, and calibration curves were linear in the range (0.662–3.23) µM with limits of detection (LOD) (0.215) or (0.053) µM and limits of quantitation (LOQ) (0.654) or (0.163) µM for the gold electrode reduction and oxidation waves, respectively. Adding different concentrations of the dye Diamond Fuchsial Basic Violet (1.0 ×10-2) M to Cr-Pic was necessary, and accordingly, the analysis data is presented in text. The method was successful in the analytical quantification of Cr-Pic in the pharmaceutical capsule. The results were evaluated in comparison with those attained by the comparative approach, and they revealed an acceptable level of agreement. The International Council of Harmonization (ICH) guidelines approved the method to be validated. The effect of Cr-Pic on oxygen waves was also studied, and it was found that a decrease in O2 waves was observed, proving the use of Cr-Pic as a useful antioxidant supplement. Conclusion: Therefore, with minimal risk to people or the environment, the new approach may be employed in the regular quality control investigation of Cr-Pic.

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Section: Introductionmentioning

confidence: 99%

A Novel Cyclic Voltamnetric Study for the Selective and Sensitive Electroanalytical Determination of Chromium Picolinate in Absence and Presence of Some Reagents in KBr Medium Using Gold Electrode. Application to Commercial Capsules

Salem,

Gomaa,

Sheribah

et al. 2024

Preprint

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Analytical Procedures for Speciation of Chromium, Aluminum, and Tin in Environmental and Biological Samples

Michalke

2016

Metallomics

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A validated method for analysis of chromium picolinate in nutraceuticals by reversed phase high performance liquid chromatography

Koll

Hoenen

Aboul‐Enein

2004

Biomedical Chromatography

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A validated high performance liquid chromatographic method was developed for the determination of chromium picolinate in pharmaceutical dosage forms. The analysis was performed at room temperature using a reversed-phase Supelcosil LC-18 (250 x 4.6 mm, 5 microm) column. The mobile phase consisted of acetonitrile:water (40:60 v/v) at a fl ow rate of 0.8 mL/min. The UV-detector was set at 264 nm. The developed method showed a good linear relationship in the concentration range from 0.125 to 12.5 microg/mL with a correlation coefficient from 0.999. The limit of detection and limit of quanti fi cation were 0.091 and 0.181 microg/mL, respectively.

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Determination of Chromium(III) Picolinate Using High Performance Liquid Chromatography-Ultraviolet Spectrophotometry

Cited by 8 publications

References 9 publications

A Novel Cyclic Voltamnetric Study for the Selective and Sensitive Electroanalytical Determination of Chromium Picolinate in Absence and Presence of Some Reagents in KBr Medium Using Gold Electrode. Application to Commercial Capsules

A Novel Cyclic Voltamnetric Study for the Selective and Sensitive Electroanalytical Determination of Chromium Picolinate in Absence and Presence of Some Reagents in KBr Medium Using Gold Electrode. Application to Commercial Capsules

Analytical Procedures for Speciation of Chromium, Aluminum, and Tin in Environmental and Biological Samples

A validated method for analysis of chromium picolinate in nutraceuticals by reversed phase high performance liquid chromatography

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