Determination of citrate in plasma protein solutions by UV-visible spectrophotometry and ion chromatography

Holden, Alexis J.; Littlejohn, David; Fell, Gordon S.

doi:10.1016/0731-7085(95)01671-6

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…Previous columns have required concentrated hydroxide eluents to elute the strongly retained citrate from the column. For example, earlier reports using a "hydroxide-selective" IonPac ® AS5 column required 75-100 mM NaOH to elute citrate [3,13]. Rapid advances in column technology for anion exchangers specifically designed for use with hydroxide eluents (i.e., "hydroxide-selective") have allowed the separation of strongly retained anions, such as citrate, at lower hydroxide concentrations and shorter retention times.…”

Section: Introductionmentioning

confidence: 98%

“…Citric acid and citrate have been assayed by ion-exchange chromatography [3,4], ion-exclusion chromatography [5,6], and reversed-phase chromatography [7] in a wide range of sample matrices, including those of pharmaceutical and biological importance. The common detection method is indirect UV [2,[8][9][10]; however, conductivity and refractive index detection have also been used.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of an assay for citric acid/citrate and phosphate in pharmaceutical dosage forms using ion chromatography with suppressed conductivity detection

DeBorba¹,

Rohrer²,

Bhattacharyya

2004

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Development and validation of an assay for citric acid/citrate and phosphate in pharmaceutical dosage forms using ion chromatography with suppressed conductivity detection

DeBorba¹,

Rohrer²,

Bhattacharyya

2004

Journal of Pharmaceutical and Biomedical Analysis

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Citric Acid Chemistry

Apelblat

2014

Citric Acid

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Electrocatalytic behaviour of citric acid at a cobalt phthalocyanine-modified screen-printed carbon electrode and its application in pharmaceutical and food analysis

2010

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Cobalt phthalocyanine-modified screen-printed carbon electrodes (CoPC-SPCEs) have been investigated as disposable sensors for the measurement of citric acid. The analyte was found to undergo an electrocatalytic oxidation process involving the Co(2+)/Co(3+) redox couple. Calibration plots were found to be linear in the range 2 mM to 2.0 M; replicate determinations of a 5.2 mM citric acid (n = 4) solution gave a coefficient of variation of 1.43%. Additions of metal ions, such as Ag(+), Pb(2+), Cu(2+), Fe(3+) and Ca(2+), were found not to interfere. The effects of hesperidin, cysteine, ethylenediaminetetraacetic acid (EDTA), ascorbic, formic, malic, malonic, tartaric, oxalic and trichloroacetic acids on the determination of citric acid were examined and, under the conditions employed, only oxalic acid and EDTA were found to give any significant interference. The sensors were evaluated by carrying out citric acid determinations on spiked and unspiked samples of an acid citrate dextrose (ACD) formulation, lime flesh and juice. For lime juice, recoveries were calculated to be 96.8% (% CV = 2.7%) for a sample fortified with 5% citric acid and for ACD 99.4% (%CV = 2.6%) when fortified at 2.30% citric acid. Further studies showed the possibility of determining citric acid concentrations in lime juice and fruit directly, without the need for an added electrolyte. These performance characteristics indicate that reliable data may be obtained for citric acid measurements in such samples. To our knowledge, this is the first report on the electrocatalytic oxidation of citric acid and its application using a CoPC-SPCE.

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Determination of citrate in plasma protein solutions by UV-visible spectrophotometry and ion chromatography

Cited by 8 publications

References 17 publications

Development and validation of an assay for citric acid/citrate and phosphate in pharmaceutical dosage forms using ion chromatography with suppressed conductivity detection

Development and validation of an assay for citric acid/citrate and phosphate in pharmaceutical dosage forms using ion chromatography with suppressed conductivity detection

Citric Acid Chemistry

Electrocatalytic behaviour of citric acid at a cobalt phthalocyanine-modified screen-printed carbon electrode and its application in pharmaceutical and food analysis

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