Determination of clenbuterol in human urine by GC–MS–MS–MS: confirmation analysis in antidoping control

Amendola, Luca; Colamonici, Cristiana; Rossi, Francesca; Botrè, Francesco

doi:10.1016/s1570-0232(02)00141-1

Cited by 71 publications

(37 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, different analytical methods have been reported for the determination of β-agonists in many biological samples, including ultra high-performance liquid chromatographymass spectrometry [8], gas chromatography-mass spectrometry [9,10], conventional liquid 3 chromatography-mass spectrometry [11][12][13], surface-enhanced Raman scattering [14], capillary electrophoresis [15], enzyme-linked immunosorbent assay [16], and immunosensors [17].…”

Section: Introductionmentioning

confidence: 99%

Copper nanoparticles applied to the preconcentration and electrochemical determination of β-adrenergic agonist: An efficient tool for the control of meat production

Regiart

Escudero

Aranda

et al. 2015

Talanta

View full text Add to dashboard Cite

Copper nanoparticles applied to the preconcentration and electrochemical determination of β-adrenergic agonist: An efficient tool for the control of meat production, Talanta, http://dx.doi.org/ 10. 1016/j.talanta.2014.12.026 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractA novel method for preconcentration and electrochemical detection of zinterol in bovine urine samples was developed. In order to improve the limit of detection, the surface of a screenprinted carbon electrode was modified with electrodeposited metal copper nanoparticles. The experimental electrodeposition optimization was performed using a central composite design (CCD), involving the variables: precursor concentration, potential and time applied. Copper nanoparticles were characterized by transmission electron microscopy, scanning electron microscopy, cyclic voltammetry, and energy dispersive X-ray spectroscopy. Mesoporous 2 shuttle-like copper oxide nanoparticles were used for the preconcentration step to avoid interferences with many compounds present in the sample matrix. The optimal working conditions for the preconcentration approach were found by means of both two-level fractional factorial and CCD designs. The obtained enhancement factor for a sample volume of 30 mL was 35 fold. The calibration curve showed linearity between 0.5-45 ng mL -1 and the limit of detection was 0.16 ng mL -1 . The intra and inter assay coefficients of variability were below 4% and 5%; respectively.

show abstract

Section: Introductionmentioning

confidence: 99%

Copper nanoparticles applied to the preconcentration and electrochemical determination of β-adrenergic agonist: An efficient tool for the control of meat production

Regiart

Escudero

Aranda

et al. 2015

Talanta

View full text Add to dashboard Cite

show abstract

“…Most screening procedures applied to b 2 -agonists are based on GC/MS analysis after derivatization (Damasceno et al, , 2002Ramos et al, 1998;Henze et al, 2001;Ventura et al, 2000), even if an increasing number of protocols using LC/MS have been published (Polettini, 1996;Kolmonen et al, 2007). Multiple-MS detection, after GC or LC separation, has also been proposed in order to improve the instrumental sensitivity and selectivity, especially for confi rmation purposes (Amendola et al, 2002;Thevis et al, 2003;Marcos et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of β₂‐agonists in human urine by fast‐gas chromatography/mass spectrometry: method validation and clinical application

Corcia

Morra

Pazzi

et al. 2010

Biomedical Chromatography

View full text Add to dashboard Cite

A fast screening protocol was developed and validated for the simultaneous determination of 15 beta(2)-agonists in human urine (bambuterol, cimbuterol, clenbuterol, fenoterol, formoterol, isoproterenol, mapenterol, metaproterenol, procaterol, ractopamine, ritodrine, salbutamol, salmeterol, terbutaline, tulobuterol). The overall sample processing includes deconjugation with enzyme hydrolysis, liquid-liquid extraction, followed by derivatization of the extract and detection of beta(2)-agonists trimethylsilyl-derivatives by fast-gas chromatography/electron impact-mass spectrometry (fast-GC/EI-MS). Sample extraction and derivatization were optimized with the purpose of improving recoveries and reaction yields for a variety of analytes with different structures simultaneously, while keeping the procedure simple and reliable. Validation parameters were determined for each analyte under investigation, including selectivity, linearity, intra- and inter-assay precision, extraction recoveries and signal to noise ratio (S/N) at the lowest calibration level. Fast-GC/MS sequences, based on the use of short columns, high carrier-gas velocity and fast temperature ramping, allow considerable reduction of the analysis time (7 min), while maintaining adequate chromatographic resolution. The overall GC cycle time was less than 9 min, allowing a processing rate of 6 samples/h. High MS-sampling rate, using a benchtop quadrupole mass analyzer, resulted in accurate peak shape definition under both scan and selected ion monitoring modes, and high sensitivity in the latter mode. The method was successfully tested on real samples arising from clinical treatments. Copyright (c) 2009 John Wiley & Sons, Ltd.

show abstract

“…[1][2][3][4] At present, routine testing techniques in athletic sports are centred around multidimensional instrumental approaches for abused-drug analysis, such as gas chromatography-mass spectrometry (GC-MS), [5] highperformance liquid chromatography (HPLC), [6,7] capillary electrophoresis (CE), [8] and matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS), [9] and may incorporate tandem mass spectrometry (MS-MS) [10] approaches for enhanced sensitivity and/or identification. These current approaches clearly involve not only expensive instruments, but also a large number of separate analytical procedures, resulting in a more-complex, timeconsuming and laborious screening procedure.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Specific Stimulant Assay Based on Molecularly Imprinted Photonic Hydrogels

et al. 2008

Adv Funct Materials

124

View full text Add to dashboard Cite

Taking theophylline and (1R,2S)‐(−)‐ephedrine as template molecules, two imprinted photonic‐hydrogel films are prepared by a combination of colloidal‐crystal and molecular‐imprinting techniques. This paper shows a new approach for rapid and handy stimulant detection with high sensitivity and specificity. One film is proposed for analogous molecule assay, another one for chiral recognition. The key point of this approach is that the imprinted photonic polymer (IPP) consists of a three‐dimensional (3D), highly‐ordered and interconnected macroporous array with a thin hydrogel wall, where nanocavities complementary to analytes in shape and binding sites are distributed. This special, bicontinuous, hierarchical structure enables this polymer to report quickly, easily, sensitively and directly a molecular recognition event without any transducers and treatments for analytes (label‐free). The inherent affinity of the nanocavities, deriving from molecular imprinting, makes these sensors highly specific to analytes, even if in a competitive environment. Their sensitive and specific responses to stimulants in buffer are determined by Bragg diffractive shifts due to the lattice change of their 3D ordered macroporous arrays resulting from their preferential rebinding to the target molecules. The measurements show that the prepared hydrogel films exhibit high sensitivity in such a 0.1 fM concentration of analytes and specificity even in a competitive urinous buffer. The reported method provides a rapid and handy approach for stimulant assay and drug analysis in athletic sports.

show abstract

Determination of clenbuterol in human urine by GC–MS–MS–MS: confirmation analysis in antidoping control

Cited by 71 publications

References 12 publications

Copper nanoparticles applied to the preconcentration and electrochemical determination of β-adrenergic agonist: An efficient tool for the control of meat production

Copper nanoparticles applied to the preconcentration and electrochemical determination of β-adrenergic agonist: An efficient tool for the control of meat production

Simultaneous determination of β₂‐agonists in human urine by fast‐gas chromatography/mass spectrometry: method validation and clinical application

Ultrasensitive Specific Stimulant Assay Based on Molecularly Imprinted Photonic Hydrogels

Contact Info

Product

Resources

About

Determination of clenbuterol in human urine by GC–MS–MS–MS: confirmation analysis in antidoping control

Cited by 71 publications

References 12 publications

Copper nanoparticles applied to the preconcentration and electrochemical determination of β-adrenergic agonist: An efficient tool for the control of meat production

Copper nanoparticles applied to the preconcentration and electrochemical determination of β-adrenergic agonist: An efficient tool for the control of meat production

Simultaneous determination of β2‐agonists in human urine by fast‐gas chromatography/mass spectrometry: method validation and clinical application

Ultrasensitive Specific Stimulant Assay Based on Molecularly Imprinted Photonic Hydrogels

Contact Info

Product

Resources

About

Simultaneous determination of β₂‐agonists in human urine by fast‐gas chromatography/mass spectrometry: method validation and clinical application