Determination of Constituents of Extract of Celtis tournefortii Lam. by LC-MS/MS, Investigation of Enzyme Inhibition, Antimicrobial and Anticancer Effects

Abstract

Phytochemicals found in extracts obtained from plants are very important bioactive constituents. In this study, phytochemicals in extract content obtained from Celtis tournefortii .Lam. tree (CT) leaves were determined by a LC-MS method. The constituents with the major concentrations was found rutin (2479.89 µg ml-1), coumarin (1241.68 µg ml-1), biochanin A (1026.42 µg ml-1), shikimic acid (477.32 µg ml-1), chlorogenic acid (300.76 µg ml-1). The suppressive effects of CT extract on the growth of pathogenic strains were studied by microdilution method. It was observed that it caused suppression on the strains in the concentration range of 2.00-8.00 µg ml-1. The inhibition effects of the extract on acetyl cholinesterase and glutathione-S transferase enzyme activities were investigated, and 50% inhibitory values of enzyme activity were found to be 13.58 and 13.86, respectively. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay method, the cell viability suppressive effects of CT leaf extract were studied in healthy skin fibroblast cells as well as ovarian, colon and brain cancer cells. It was observed that they created a 42%, 4.27%, and 14.29% suppression in cells, respectively.

A comprehensive account on ethnobotany, phytochemistry and pharmacological insights of genus Celtis

“… Flavonolignan Silibinin C. tournefortii Young twigs Methanol–dichloromethane extract HPLC-TOF/MS Turkey [ 114 ] 122. Isoflavone Biochanin A C. tournefortii Fruits, Leaves & Young twigs Methanol–dichloromethane extract HPLC-TOF/MS Turkey [ 114 ] C. tournefortii Leaves Methanol extract LC-MS/MS Mardin [ 125 ] 123. Isoflavone glycoside Genistin C. iguanaea Leaves Dichloromethane extract ESI-MS Brazil [ 121 ] Organic acids 124.…”

“… Aliphatic dicarboxylic acid Sebacic acid C. adolphi-friderici Roots Acetone extract EI-MS, H-NMR Cameroon [ 103 ] 130. Aliphatic dicarboxylic acid Shikimic Acid C. tournefortii Leaves Methanol extract LC-MS/MS Mardin [ 125 ] 131. Aliphatic dicarboxylic acid Succinic acid C. tessmannii Roots Methanol extract NMR, UV, IR, MS, GC-MS Cameroon [ 97 ] 132.…”

Green-Synthesized Characterization, Antioxidant and Antibacterial Applications of CtAC/MNPs-Ag Nanocomposites

“…Dardagan, also known by its scientific name Celtis tournefortii , is a species of flowering plant belonging to the Cannabaceae family. Native to the Middle East, particularly Iran and Iraq, the Dardagan is a small to medium-sized species that can reach heights of up to 15 m. It is known for its distinctive appearance, with dark green, oval leaves and small, round fruits that turn purplish-black when ripe [ 31 , 32 ].…”