Determination of Copper and Zinc in Biological Material

Parker, Mary M.; Humoller, Fred L.; Mahler, Delmar J.

doi:10.1093/clinchem/13.1.40

Cited by 268 publications

(29 citation statements)

References 4 publications

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“…Samples of blood were collected for measurement of serum zinc concentration by atomic absorption spectrophotometry. Hepatic zinc concentration was measured in acid-digested samples by atomic absorption spectrophotometry (9). The experiments followed the criteria set by our institution for the care and use of laboratory animals.…”

Section: Methodsmentioning

confidence: 99%

Influence of dietary zinc on hepatic collagen and prolyl-hydroxylase activity in alcoholic rats

Giménez¹,

Parés²,

Caballería³

et al. 1990

Journal of Hepatology

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Section: Methodsmentioning

confidence: 99%

Influence of dietary zinc on hepatic collagen and prolyl-hydroxylase activity in alcoholic rats

Giménez¹,

Parés²,

Caballería³

et al. 1990

Journal of Hepatology

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“…Arsenic was estimated using a Hydride Vapor Generation System (Perkin Elmer model MHS-10, Norwalk, CT, USA) fitted with an atomic absorption spectrophotometer (AAS, Perkin Elmer model AAnalyst 100, Norwalk, CT, USA). Zinc, copper and magnesium contents were also measured in the digested tissue samples using AAS [18].…”

Section: Chemicalsmentioning

confidence: 99%

Monoisoamyl 2, 3‐Dimercaptosuccinic Acid (MiADMSA) Demonstrates Higher Efficacy by Oral Route in Reversing Arsenic Toxicity: A Pharmacokinetic Approach

Flora

Bhadauria

Pachauri

et al. 2011

Basic Clin Pharma Tox

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Monoisoamyl DMSA (MiADMSA), a lipophilic chelating agent has emerged as a promising drug for the treatment of arsenic. The present study aimed at exploring the optimum dose and route of administration for achieving maximum arsenic elimination with minimal side effects. We also carried out a pharmacokinetic analysis of this drug to support arsenic chelation. Rats were exposed to arsenic (25 ppm) for 6 months and later received MiADMSA (50 or 100 mg ⁄ kg) orally and via i.p. route for 5 days. Oxidative stress parameters and arsenic levels in soft tissues, liver function test and histopathology of liver and kidney were performed. Plasma kinetic of MiADMSA (plasma-free drug and total drug) at 50 and 100 mg ⁄ kg p.o. was carried out. Arsenic exposure resulted in significant oxidative stress and hepatotoxicity. MiADMSA at 50 mg ⁄ kg dose administered orally provided about 45% and 75% protection against oxidative stress and in lowering body arsenic burden, respectively, against 25% and 40% via i.p. route. Pharmacokinetic analysis supported prolonged availability of the drug through oral administration. Collectively, these findings led us to conclude that oral administration of MiADMSA was more effective than intraperitoneal administration and that the minimum effective dose with least side effects was 50 mg ⁄ kg.Chelation therapy has been the basis for the medical therapy for metal poisoning. Chelating agent decreases metal toxicity by depleting the body burden of toxic metal by enhancing their excretion in addition to preventing these metals from binding to cellular targets [1]. The efficiency of chelating agent depends on multiple factors such as tissue distribution, time delay between metal intoxication and administration of the chelating agent, level of exposure and the dose ⁄ route administered. It has been known that DMSA can be used as an antidote for the treatment of arsenic toxicity; however, its efficacy against chronic arsenicosis is very limited [2]. Investigations have shown monoalkyl esters of DMSA to be far superior than DMSA, in mobilizing lead [3], cadmium [4,5] and mercury [6]. The efficiency increased with the number of carbon atoms in the alkyl group of DMSA monoester, and the best results were obtained with Monoisoamyl DMSA (MiADMSA). As treatment with DMSA is an accepted means for reducing the body burden of arsenic, the newer and more efficient monoesters, especially MiADMSA, deserve further attention [7]. MiADMSA is a water-soluble, lipophilic chelating agent. It is capable of chelating intracellular arsenic because of extensive cellular distribution. A large number of reports are available on the therapeutic efficacy of MiADMSA. Pande et al. [8] found that MiADMSA was effective in prevention and treatment of acute lead intoxication. Walker et al. [3] reported that MiADMSA reduced brain lead concentration by 75% when compared to 35% with DMSA.

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“…Arsenic was estimated using a Hydride Vapour Generation System (Perkin Elmer model MHS-10) fitted with an atomic absorption spectrophotometer (AAS, Perkin Elmer model AAnalyst 100). Zinc, copper and magnesium contents were also measured in the digested tissue samples using AAS (Parker et al, 1967).…”

Section: Metal Estimationmentioning

confidence: 99%

Concomitant administration of Moringa oleifera seed powder in the remediation of arsenic‐induced oxidative stress in mouse

Gupta

Dubey

Kannan

et al. 2007

Cell Biology International

119

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Contamination of ground water by arsenic has become a cause of global public health concern. In West Bengal, India, almost 6 million people are endemically exposed to inorganic arsenic by drinking heavily contaminated groundwater through hand-pumped tube wells. No safe, effective and specific preventive or therapeutic measures for treating arsenic poisoning are available. We recently reported that some of the herbal extracts possess properties effective in reducing arsenic concentration and in restoring some of the toxic effects of arsenic in animal models. Moringa oleifera Lamarack (English: Horseradish-tree, Drumstick-tree, Hindi: Saijan, Sanskrit: Shigru) belongs to the Moringaceae family, is generally known in the developing world as a vegetable, a medicinal plant and a source of vegetable oil. The objective of the present study was to determine whether Moringa oleifera (M. oleifera) seed powder could restore arsenic induced oxidative stress and reduce body arsenic burden. Exposure to arsenic (2.5 mg/kg, intraperitoneally for 6weeks) led to a significant increase in the levels of tissue reactive oxygen species (ROS), metallothionein (MT) and thiobarbituric acid reactive substance (TBARS) which were accompanied by a decrease in the activities in the antioxidant enzymes such as superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) in mice. Arsenic exposed mice also exhibited liver injury as reflected by reduced acid phosphatase (ACP), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) activities and altered heme synthesis pathway as shown by inhibited blood delta-aminolevulinic acid dehydratase (delta-ALAD) activity. Co-administration of M. oleifera seed powder (250 and 500 mg/kg, orally) with arsenic significantly increased the activities of SOD, catalase, GPx with elevation in reduced GSH level in tissues (liver, kidney and brain). These changes were accompanied by approximately 57%, 64% and 17% decrease in blood ROS, liver metallothionein (MT) and lipid peroxidation respectively in animal co-administered with M. oleifera and arsenic. Another interesting observation has been the reduced uptake of arsenic in soft tissues (55% in blood, 65% in liver, 54% in kidneys and 34% in brain) following administration of M. oleifera seed powder (particularly at the dose of 500 mg/kg). It can thus be concluded from the present study that concomitant administration of M. oleifera seed powder with arsenic could significantly protect animals from oxidative stress and in reducing tissue arsenic concentration. Administration of M. oleifera seed powder thus could also be beneficial during chelation therapy with a thiol chelator.

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Determination of Copper and Zinc in Biological Material

Cited by 268 publications

References 4 publications

Influence of dietary zinc on hepatic collagen and prolyl-hydroxylase activity in alcoholic rats

Influence of dietary zinc on hepatic collagen and prolyl-hydroxylase activity in alcoholic rats

Monoisoamyl 2, 3‐Dimercaptosuccinic Acid (MiADMSA) Demonstrates Higher Efficacy by Oral Route in Reversing Arsenic Toxicity: A Pharmacokinetic Approach

Concomitant administration of Moringa oleifera seed powder in the remediation of arsenic‐induced oxidative stress in mouse

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