Determination of Coxiella burnetii in bovine foetuses using PCR and immunohistochemistry

Özkaraca, Mustafa; Çeribaşı, Songül; Çeribaşı, Ali Osman; Kiliç, Ayşe; Altun, Serap Kılıç; Çomaklı, Selim; Öngör, Hasan

doi:10.17221/138/2015-vetmed

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…Compatible with these fi ndings, the lesions detected in our study were mild. We found interstitial lymphocyte and mononuclear cell infi ltrations foci [11,14] and necrosis of hepatocytes in the liver [25] and a mild interstitial pneumonia in the lungs [24,25].…”

Section: Discussionmentioning

confidence: 77%

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Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods

et al. 2018

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Brucellosis and Q fever, two zoonoses, are important causes of abortion in ruminants, as well as economically significant diseases caused by a gram-negative bacterium. Determination of these diseases is therefore of great importance. In this study, the organs of 35 naturally infected and aborted ovine fetuses were examined for the presence of changes resulting from infections by Brucella melitensis and Coxiella burnetii, according to macroscopic, bacteriological, histopathological and immunohistochemical methods. B. melitensis was observed in 21 cases, and C. burnetii was observed in 8 cases of the aborted ovine fetuses, and these were determined with immunohistochemical methods. Brucellosis was observed in 18 of the aborted ovine fetuses, and this was determined by microbiological methods. Negative (-) results were found for all of the other fetuses. The Brucella antigen was determined to be localized as intracytoplasmic in mainly alveolar macrophages, bronchi, bronchioles, glandular epithelial cells around bronchial glands, neutrophils, hepatocytes and Kupffer cells. The Coxiella antigen was found to be localized in the alveolar macrophages in the lungs, bronchi, bronchioles and alveolus, and in the cytoplasms of bronchial gland epithelial cells, and in the cytoplasms of hepatocytes and Kupffer cells in the liver. Immunohistochemical and microbiological diagnoses of brucellosis and coxiellosis were compared; it was concluded that immunohistochemical methods were more safely applied than microbiological methods.

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Section: Discussionmentioning

confidence: 77%

“…Also, due to frequently occurring cross-reactions, serologic tests may yield false-negative results. Consequently, in cases of infected abortus, the determination of Brucella and Coxiella antigens with the highly sensitive and specifi c immunohistochemical methods is preferred [10][11][12][13][14][15].…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods

et al. 2018

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“…The tissues were homogenized at a speed of three meters per second (3 m/s) for 90 s (s). DNA extraction from the homogenates was conducted using the Qiagen DNeasy ® blood and tissue kit as previously described by [16]. The homogenates were centrifuged for 15 min at 4000 rpm and 200 µL of the supernatant transferred to 2 mL centrifuge tubes.…”

Section: Dna Extraction and Pcr For Detection Of C Burnetiimentioning

confidence: 99%

Q Fever: Seroprevalence, Risk Factors in Slaughter Livestock and Genotypes of Coxiella burnetii in South Africa

et al. 2021

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Q fever is a neglected zoonosis in South Africa, causing significant losses in livestock and game animals through reproductive disorders. However, there are limited studies on the extent of Coxiella burnetii infections in livestock in South Africa. Further, there is also lack of knowledge about the types of C. burnetii strains that are currently circulating in the country. Therefore, a cross-sectional, abattoir-based study was conducted to determine the seroprevalence of C. burnetii and associated risk factors, and to characterize C. burnetii strains from slaughter livestock at red meat abattoirs in Gauteng, South Africa. Of the 507 animals tested, 6.9% (95% CI: 4.9–9.5%) were positive for antibodies against C. burnetii. The seroprevalence was 9.4% (31/331) in cattle, 4.3% (3/69) in sheep, and 0.9% (1/107) in pigs. Out of the 63 tissue samples from 35 seropositive animals including material from two sheep aborted fetuses from Mangaung district (Free State province), 12.7% (8/63) tested positive by IS1111 PCR. Genotyping of the eight PCR-positive tissues from eight animals by MLVA revealed two novel genotypes, not available in Coxiella MLVA databases. It is concluded that slaughter animals pose a risk of exposing abattoir and farm workers to C. burnetii in South Africa.

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Ruminant Abortus Vakalarında Coxiella burnetii’nin Real Time PCR ile Araştırılması

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2018

Harran Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Q fever dünyanın birçok ülkesinde görülen önemli zoonotik infeksiyonlardan biridir. Etkenin kültürünün zor ve uzun zaman alıcı olmasının yanında Biyogüvenlik seviyesi-3 (Biosafety level-3) BSL-3 koşullarında gerçekleştirilmesi gerekliliği, hastalığın teşhisinin geniş oranda moleküler ve serolojik olmasını sağlamıştır. Bu çalışmada, ruminantlarda abortusa neden olan bu önemli patojenin rutin teşhiste Real-Time PCR ile hızlı ve doğru bir şekilde saptanması ve Şanlıurfa yöresinde hastalığın varlığının belirlenmesi amaçlandı. Çalışmada Harran Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalına teşhis amaçlı getirilmiş olan ve -80 ºC'de saklanan toplam 202 adet sığır, keçi ve koyun atık materyali ve çalışma döneminde gelen 25 atık materyali Real-Time PCR tekniği ile incelendi. Bu amaçla içinde pozitif kontrol içeren Coxiella burnetii Real-Time PCR kiti kullanıldı. Ayrıca test edilen C. burnetii suşları arasında son derece korumalı bir bölge olan C. burnetii com 1 geninin74-bp lik bir fragmentini amplifiye eden primer çiftleri kullanılarak klasik PCR yapıldı ve elde edilen amplikonlar agaroz jelde görüntülendi. Çalışmada incelenen toplam 227 adet atık materyalinden 4 adedi (%1,8) hem Real-Time PCR hem de klasik PCR ile pozitif bulundu. Çalışmanın sonucunda önemli bir zoonoz olan bu ajanın hızlı ve güvenli bir şekilde tespit edilebildiği ve gerekli kontrol önlemlerinin alınmasına katkıda bulunacağı kanaatine varıldı.

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Determination of Coxiella burnetii in bovine foetuses using PCR and immunohistochemistry

Cited by 3 publications

References 25 publications

Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods

Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods

Q Fever: Seroprevalence, Risk Factors in Slaughter Livestock and Genotypes of Coxiella burnetii in South Africa

Ruminant Abortus Vakalarında Coxiella burnetii’nin Real Time PCR ile Araştırılması

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