Determination of cysteine and glutathione in plasma and blood by liquid chromatography with electrochemical detection using a chemically modified electrode containing cobalt phthalocyanine

Halbert, Mary Kim; Baldwin, Richard P.

doi:10.1016/0378-4347(85)80133-x

Cited by 77 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The electrocatalytic oxidation of cysteine at a phthalocyanine tetrasulfonate mediated graphite electrode was reported by Zagal et al, [19]. Halbert and Baldwin developed carbon paste electrode containing cobalt phthalocyanine for electrocatalysis and amperometric detection following liquid chromatography of cysteine, N-acetyl cysteine and homocysteine [20,21]. Cysteine and reduced glutathione were electrocatalytically oxidized at graphite electrodes modified with tetracynao-p-quinodimethanetetrathiafulvalene, and 1,1 0 -dimethyl ferrocene [22].…”

Section: Introductionmentioning

confidence: 96%

Electrocatalytic Oxidation of Sulfhydryl Compounds at Ruthenium(III) Diphenyldithiocarbamate Modified Carbon Paste Electrode

Nalini

Narayanan

1998

Electroanalysis

View full text Add to dashboard Cite

An aqueous insoluble new redox mediator ruthenium(III)-diphenyldithiocarbamate has been used as a modifier to prepare a carbon paste electrode. The modified electrode was characterized by cyclic voltammetry by varying the pH of the medium and also the scan rate. The electrode was scanned between 0.0 V and þ0.8 V in 0.1 M KNO 3 medium. Anodic and cathodic peaks were observed at þ0.38 V and þ0.22 V, respectively, for a scan rate of 100 mV/s. The modified electrode is found to oxidize electrocatalytically cysteine and glutathione. Conditions such as pH and scan rate have been optimized for the determination of both compounds. Linear response for cysteine is in the range of 0.00-15.20 mg/L, with a correlation coefficient (r), of 0.9992. Standard deviation for 10 repetitive determinations was found to be 0.0756. The linear range for glutathione is 0.00-30.40 mg/L, with a value of 0.9995 for r, and the standard deviation is 0.1449. The stability and reproducibility of the electrode for the determination of cysteine and glutathione were also discussed. Hydrodynamic voltammogram was also recorded for the electrocatalytic oxidation of the sulfhydryl compounds. Amperometric determination and studies on the stability of the electrode for a period of 10 days were also carried out.

show abstract

Section: Introductionmentioning

confidence: 96%

Electrocatalytic Oxidation of Sulfhydryl Compounds at Ruthenium(III) Diphenyldithiocarbamate Modified Carbon Paste Electrode

Nalini

Narayanan

1998

Electroanalysis

View full text Add to dashboard Cite

show abstract

“…The phthalocyanine reduction peak (IcPTHAL) was found to be unaffected by the solution conditions studied here. reported by Halbert and Baldwin [33]. The resulting plot of H202 concentration versus current shows that it is linearly related to the concentration of the analyte over the range studied.…”

Section: Voltammetric Studies On H202 At Unmodified and Copc-spcesmentioning

confidence: 73%

Voltammetric and photoelectron spectral elucidation of the electrocatalytic oxidation of hydrogen peroxide at screen‐printed carbon electrodes chemically modified with cobalt phthalocyanine

et al. 1995

View full text Add to dashboard Cite

The interplaying mechanisms involved in the electrocatalytic oxidation of hydrogen peroxide (H202) have been studied using cyclic and differential-pulse voltammetry and X-ray photoelectron spectroscopy (XPS). Cobalt phthalocyanine (CoPC) modified screen-printed carbon electrodes (SPCEs) were used as base transducers in the voltammetric studies, and for XPS experiments a mixture of the printing ink containing CoPC was coated onto a steel stub. These investigations have shown that CoPC is initially in the Co2+ oxidation state, and that in plain supporting electrolytes it is subsequently electrochemically oxidized to Co3+. A postulated sequence of events for the mediated oxidation of H202 at CoPC-SPCEs has been derived from the XPS and voltammetric data generated.

show abstract

“…Another interesting approach is to add them directly to the paste as precursors for metal film electrodes which renders plating with a solution of the metal obsolete (e.g., [134]). Metal complexes: Similarly to metal oxides, these compounds can be exploited for electrocatalytic (mediating) actions to facilitate electron transport between analyte and electrode surface [135][136][137]. Ferrocenes, …”

Section: Types Of Modifiersmentioning

confidence: 99%

Carbon Paste Electrodes

Švancara

Kalcher

2015

Advances in Electrochemical Sciences and Engineering

View full text Add to dashboard Cite

Determination of cysteine and glutathione in plasma and blood by liquid chromatography with electrochemical detection using a chemically modified electrode containing cobalt phthalocyanine

Cited by 77 publications

References 17 publications

Electrocatalytic Oxidation of Sulfhydryl Compounds at Ruthenium(III) Diphenyldithiocarbamate Modified Carbon Paste Electrode

Electrocatalytic Oxidation of Sulfhydryl Compounds at Ruthenium(III) Diphenyldithiocarbamate Modified Carbon Paste Electrode

Voltammetric and photoelectron spectral elucidation of the electrocatalytic oxidation of hydrogen peroxide at screen‐printed carbon electrodes chemically modified with cobalt phthalocyanine

Carbon Paste Electrodes

Contact Info

Product

Resources

About