Determination of D-Glucose in Corn Sirups

Abstract

There has heen need for a quantitative method for the determination of D-glucose in the presence of other sugars, and particularly for the determination of Dglucose in corn sirups. The present work shows that glucose dehydrogenase can be conveniently employed for the direct stoichiometric determination of D-glucose in a variety of corn sirups.

Physiological Disposition and Metabolic Fate of Infused Pentoses in Man

“…In order to determine accurately the concentrations of glucose and of pentose or other hexose when glucose and a second sugar were present simultaneously in blood or urine, we have adapted an industrial method designed to determine true glucose in mixtures of sugars (20). This method employs glucose oxidase to oxidize glucose selectively.…”

“…This method employs glucose oxidase to oxidize glucose selectively. Glucose can be measured by titration of the product, gluconic acid (20), by measurement of oxygen consumption (21), or by change in reducing power of the mixture, as in the present paper. Furthermore,-once glucose is destroyed, pentose can be determined by the standard orcinol reaction without glucose interference.…”

Analytical Methods for Carbohydrates

“…316 The consumption of oxygen can be measured manometrically. The lactone may be converted into D-gluconic acid, which is titrated with standard alkali.…”

Sapid Components in Carrot

“…Determination of total reducing sugars were made with Bertrand's method. Determination of glucose and maltose were made with glucose oxidase method by the procedure of Whistler et al (1953).…”