Determination of daily dietary intake of chromium by duplicate diet sampling:In vitroavailability study

Abstract

Intake of chromium was estimated using a duplicate diet sampling method of 108 meals (36 breakfasts, 36 lunches and 36 dinners) from the restaurant of the Hospital of Motril (S.E. Spain), corresponding to 36 consecutive days. Total and dialyzable Cr levels were measured by a validated electro-thermal atomic absorption spectrometry (ETAAS) method. A mean Cr fraction of 26 +/- 12 microg meal (-1) was found. The Cr uptake from meals was directly and significantly (p < 0.001) correlated with their macronutrient (carbohydrates, fibre and protein) content. Cereals and cereal by-products, legumes, dry fruits, meat, potatoes, dairy products and seafood are the primary sources of Cr. The mean Cr fraction dialyzed through dialysis tubing was 1.2 +/- 1.1 microg meal(-1) (4.6 +/- 3.8% as mean Cr dialysability). Cr intake for breakfasts was significantly lower (p < 0.001). A correlation between the logarithmic data of total and dialyzable fraction of Cr in meals (p = 0.020) was found and dialysis ratio enhancement and, therefore, bioavailability increased with total Cr. The dialysed element content present in meals was significantly correlated with fibre, protein, Fe, Na, I, F, sodium, ascorbic acid and vitamin A levels (p < 0.05). At Fe contents in meals higher than congruent with7.5 mg meal(-1) the net absorption of Cr decreased significantly. The mean Cr daily dietary intake (DDI) was 77 +/- 17 microg day (-1) which indicates that no adverse effects in relation to Cr nutrition (deficiency or toxicity) should occur in individuals from the area.

Iron Dialyzability from Hospital Duplicate Meals: Daily Intake

“…In the present study, total chromium levels of duplicate meals (27) were correlated with dialyzable iron fractions. The positive effect reported could be related with the fact that, although the chromium shares some similarities with the iron absorption pathway (2), at low chromium levels (26 μg/meal in analyzed meals), this element stimulates the iron dialysis.…”

“…The technique measures the iron fraction dialyzed from a sample under simulated gastrointestinal conditions and, therefore, available for absorption (27)(28).…”

“…The simulated gastrointestinal digestion procedure and the in vitro absorption estimation were done according to that previously reported (27)(28). The technique measures the iron fraction dialyzed from a sample under simulated gastrointestinal conditions and, therefore, available for absorption (27)(28).…”

“…The estimation of contents and corresponding DDIs of 51 nutrients and energy in analyzed meals was done as reported elsewhere (27).…”

Dialysability of Magnesium and Calcium from Hospital Duplicate Meals: Influence Exerted by Other Elements

“…The detailed description of the composition and previous treatment to the Mg and Ca analysis of the analysed duplicate meals consumed daily was reported elsewhere [30]. The treatment of duplicate meals as a previous step to obtain the analytical dissolution for the Mg and Ca determination by FAAS was published elsewhere [30,31].…”