Determination of degree of methylation of food pectins by chromatography

Luzio, Gary A.; Cameron, Randall G.

doi:10.1002/jsfa.6061

Cited by 8 publications

(6 citation statements)

References 34 publications

(60 reference statements)

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“…In a first experiment, OGs were analyzed in the hydrolysis products of commercial citrus pectins (degree of methylesterification [DM] 60) (18) digested for 20 h with Aspergillus aculeatus PG. OGs were separated using hydrophilic interaction liquid chromatography (HILIC) (19)(20)(21), and characterized using high-resolution mass spectrometry (HR-MS) in negative mode.…”

Section: Development Of a Sensitive Separative Methods For Og Charactementioning

confidence: 99%

Oligogalacturonide production upon Arabidopsis thaliana – Botrytis cinerea interaction

Voxeur

Habrylo

Guénin

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

122

123

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Despite an ever-increasing interest for the use of pectin-derived oligogalacturonides (OGs) as biological control agents in agriculture, very little information exists—mainly for technical reasons—on the nature and activity of the OGs that accumulate during pathogen infection. Here we developed a sensitive OG profiling method, which revealed unsuspected features of the OGs generated during infection of Arabidopsis thaliana with the fungus Botrytis cinerea. Indeed, in contrast to previous reports, most OGs were acetyl- and methylesterified, and 80% of them were produced by fungal pectin lyases, not by polygalacturonases. Polygalacturonase products did not accumulate as larger size OGs but were converted into oxidized GalA dimers. Finally, the comparison of the OGs and transcriptomes of leaves infected with B. cinerea mutants with reduced pectinolytic activity but with decreased or increased virulence, respectively, identified candidate OG elicitors. In conclusion, OG analysis provides insights into the enzymatic arms race between plant and pathogen and facilitates the identification of defense elicitors.

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Section: Development Of a Sensitive Separative Methods For Og Charactementioning

confidence: 99%

Oligogalacturonide production upon Arabidopsis thaliana – Botrytis cinerea interaction

Voxeur

Habrylo

Guénin

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

122

123

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“…Luzio and R.G. Cameron's method for improvement, shortening the sample pretreatment and injection time on this basis [24]. Then, de-esterified pectin was precipitated by adding CuSO 4 or isopropanol, as in the literature [17].…”

Section: Determination Of Dm and Dac By Hplcmentioning

confidence: 99%

“…Additionally, 1 H-NMR has been used in determining the DM and DAc of pectin, but this method is seldom used because of its higher cost [21,22]. There were some reports concentrated on the determination of DM by different methods and discussed advantages and limitations of the applied methods [17,[23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Analytical Methods for Determining Methylesterification and Acetylation of Pectin

Wang

Liu

et al. 2021

Applied Sciences

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The esterification of galacturonic acid with methanol and/or acetic acid is important for the structural analysis of pectin. Although several methods have been reported for determining the degree of methylesterification (DM) and acetylation (DAc), the present study compares and optimizes three methods (titration, FT-IR and HPLC) using commercial citrus pectin (CP). Our results showed that the DM of CP was 47.0%, 47.6% and 48.0% as determined by titration, FT-IR and HPLC, respectively, suggesting that DM determination is nearly identical using any of these methods. However, the titration approach requires more sample than the other two. HPLC showed that the DAc of CP was 1.6%, an approach that can be used to determine the DM and DAc of pectin simultaneously. Here, we simplified and optimized sample treatment for HPLC analysis and compared it with the reported literature. Our results provide useful information for choosing appropriate methods for determining the DM and DAc of pectin based on various sample properties and experimental conditions.

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“…DM was computed via the ratio of conductivity/RI voltage. Reference pectin DM values were determined by a modified chromatography method (Luzio & Cameron, 2013).…”

Section: Dm and Mw By Sec-conductivity-malls-rimentioning

confidence: 99%

Pectin homogalacturonans: Nanostructural characterization of methylesterified domains

et al. 2015

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Determination of degree of methylation of food pectins by chromatography

Abstract: The chromatography procedure described in this paper provides an alternative method for determination of DM of food pectins for accurate and efficient analysis when confronted with limited quantities or large numbers of samples.

Cited by 8 publications

References 34 publications

Oligogalacturonide production upon Arabidopsis thaliana – Botrytis cinerea interaction

Oligogalacturonide production upon Arabidopsis thaliana – Botrytis cinerea interaction

Comparison of Analytical Methods for Determining Methylesterification and Acetylation of Pectin

Pectin homogalacturonans: Nanostructural characterization of methylesterified domains

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