Determination of desmosine, isodesmosine, and other amino acids by liquid chromatography with electrochemical detection following precolumn derivatization with naphthalenedialdehyde/cyanide

Lunte, Susan M.; Mohabbat, Tariq; Wong, Osborne S.; Kuwana, Theodore

doi:10.1016/0003-2697(89)90380-1

Cited by 38 publications

(15 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This reagent reacts with primary amines in the presence of cyanide to produce cyano[f]benzoisoindole (CBI) derivatives. Generally, these derivatives are more stable than their OPA counterparts and are both fluorescent and electroactive [32,33]. In CE utilizing NDA-derivatives, fluorescence detection is often used.…”

Section: Introductionmentioning

confidence: 99%

Analysis of amino acids by capillary zone electrophoresis with electrochemical detection

2002

View full text Add to dashboard Cite

The precapillary derivatization of 20 amino acids with naphthalene-2,3-dicarboxaldehyde (NDA) and CN(-) was investigated. All these derivatized amino acids could be oxidized on the carbon fiber microdisk bundle electrode except proline. Capillary zone electrophoresis with electrochemical detection was employed for the analysis of 19 amino acids. The optimum conditions of separation and detection were borate, pH 9.48, for the electrolyte, 18 kV for the separation voltage and 1.15 V versus a saturated calomel electrode for the detection potential. Limits of detection of concentration or mass for individual amino acids were between 1.7 x 10(-7) and 1.8 x 10(-6) mol/L or 84 and 893 amol (according to the signal-to-noise ratio of 3) for the injection voltage of 6 kV and injection time of 10 s. The relative standard deviations were between 0.80 and 2.3% for the migration times and 1.4 and 6.4% for the electrophoretic peak currents. From a mixture of 19 amino acids, 10 amino acids (Arg, Lys, Orn, Try, Ser, Ala, Gly, Cys, Glu, Asp) could be well separated. The other 9 amino acids appeared on three electrophoretic peaks. From the samples, in which the nine amino acids do not exist simultaneously, some of them could also be detected. The method was applied to the determination of amino acids in beer by the standard addition method. The recovery for the amino acids in beer was 91-109%.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of amino acids by capillary zone electrophoresis with electrochemical detection

2002

View full text Add to dashboard Cite

show abstract

“…Using 0.1 M phosphate buffer-ACN containing 20 mM SDS (pH 4.5) the detection limit of these amino acids was 0.1 lg/mL. Particularly interesting was the method developed by Lunte et al [72] who derivatized desmosines with napthalenedialdheyde/cyanide (NDA/CN) using electrochemical detection to determine the cyano[f]benzoisoindole (CBI) derivatives. The combination of this derivatization with electrochemical detection was found to be linear over three orders of magnitude and detection limit was 100 fmol.…”

Section: Hplcmentioning

confidence: 99%

Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

Viglio

Annovazzi

Luisetti

et al. 2007

J of Separation Science

View full text Add to dashboard Cite

Desmosines are crosslinking amino acids unique to mature elastin in humans. Owing to this unicity, they have been discussed as potentially attractive indicators of connective tissue disorders whose clinical manifestations are mostly the result of elastin degradation. This review covers advances in immunochemical, chromatographic, and electrophoretic procedures applied in the last 25 years to detect and quantitate these crosslinksin a variety of biological samples. Recent applications of CE with LIF detection (CE-LIF) for investigating the content of desmosines in different fluids will also be discussed.

show abstract

“…It is still completely unknown whether these pericliondrialperiosteal elastic fibres contribute to the mechanical competence of the collagen network in resisting to both physiological and pathological loading [2,5,50], and in regulating long bone growth [6,12,22,31,33]. Some recent observations of a dramatic reduction of perichondrial-periosteal elastic fibres in human and bovine Marfan syndromes led us to hypothesize that skeletal overgrowth, typical of this disorder, may be related to alterations of elastic fibres and of their microfibrillar component [19,20,27], the Marfan syndrome being a genetric disorder caused by mutations of the fibrillin-1 gene, a major constituent of elastin-associated microfibrils [ 131.…”

Section: Biochemistrymentioning

confidence: 99%

“…Extensive attention has been given to the latter, i.e., vascular supply, perichondrial-periosteal connective tissue and muscular activity [3,6,23,25,32,33,35,49,51,53]. It has been shown that experimental interference with the subchondral vascularisation has no effect on longitudinal skeletal growth; moreover, the legation of nutrient artery has only limited curbing effect on bones [31].…”

Section: Introductionmentioning

confidence: 99%

“…The thick perichondriumperiosteum of young bones has an inherent elasticity and probably exerts a constant mechanical restraint at both ends [14]. It has been hypothesized that the biomechanical mechanism of longitudinal bone growth consists of the growth plate chondrocytes proliferation placing tension upon the perichondrium-periosteum [23, 25,33,491. In fact, observations show that release of perichondrial-periosteal tension by transverse section of the periosteum [12,14,23,25,32.35] or by amputation of growing long bone [49] might remove the restraint, allowing the plates to grow more rapidly.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

DL‐penicillamine induced alteration of elastic fibers of periosteum‐perichondrium and associated growth inhibition: an experimental study

Gigante

Chillemi

Quaglino

et al. 2001

Journal Orthopaedic Research

View full text Add to dashboard Cite

Perichondrium-periosteum, being of collagen and elastic fiber, is regarded as a bone growth regulating factor. The aim of the present study was to investigate the role of collagen and elastic fibers on bone growth, by interfering with the fiber assembly in growing chicks upon administration of oL-penicillamine ( DL-PNA).Our findings demonstrated that DL-PNA determined relevant modifications in the perichondrium-periosteum, as shown by histochemical, histomorphometrical.biochemica1 and ultrastructural analysis. This chemical has been shown to inhibit the formation of desmosine cross-links in elastin and to induce an increase of elastin associated microfibrils. On the contrary, the collagen network and the biochemical collagen markers were not affected. These changes resulted in a dramatically reduced growth of long bones in comparison with control. Perichondrial-periosteal regulation of bone growth may be mediated by mechanical and biological factors. This study demonstrates a microstructural change in the perichondriurn-periosteum with decreased elastin and increased elastic microfibrils content in penicillamine treated chicks. The mechanism linking changes in the perichondrium-periosteum with altered growth still needs to be elucidated.

show abstract

Determination of desmosine, isodesmosine, and other amino acids by liquid chromatography with electrochemical detection following precolumn derivatization with naphthalenedialdehyde/cyanide

Cited by 38 publications

References 16 publications

Analysis of amino acids by capillary zone electrophoresis with electrochemical detection

Analysis of amino acids by capillary zone electrophoresis with electrochemical detection

Progress in the methodological strategies for the detection in real samples of desmosine and isodesmosine, two biological markers of elastin degradation

DL‐penicillamine induced alteration of elastic fibers of periosteum‐perichondrium and associated growth inhibition: an experimental study

Contact Info

Product

Resources

About