Determination of dexamethasone acetate in cream by HPLC

Garcia, Cássia Virgínia; Breier, Ana Rita; Steppe, Martin; Schapoval, Elfrides E.S.; Oppe, Tércio Paschke

doi:10.1016/s0731-7085(02)00695-7

Cited by 36 publications

(12 citation statements)

References 5 publications

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“…[12][13][14] The chromatographic conditions 15,16 were determined using a column C18 (Spherisorb ® ). The limits of detection and quantification were 0.5 µg/mL and 1.25 µg/mL, respectively.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

Cannabinoid derivate-loaded PLGA nanocarriers for oral administration: formulation, characterization, and cytotoxicity studies

Martín-Banderas¹,

Álvarez-Fuentes²,

Durán‐Lobato³

et al. 2012

IJN

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CB13 (1-Naphthalenyl[4-(pentyloxy)-1-naphthalenyl]methanone)-loaded poly(lactic-co-glycolic acid) nanoparticles (NPs) were produced by nanoprecipitation and tested for their in vitro release behavior and in vitro cytotoxicity assays. The effects of several formulation parameters such as polymer type, surfactant concentration, and initial drug amount were studied. NPs had a particle size 90-300 nm in diameter. Results obtained show that the main influence on particle size was the type of polymer employed during the particle production: the greater the hydrophobicity, the smaller the particle size. In terms of encapsulation efficiency (%), high values were achieved (∼68%-90%) for all formulations prepared due to the poor solubility of CB13 in the external aqueous phase. Moreover, an inverse relationship between release rate and NP size was found. On the other hand, low molecular weight and low lactide content resulted in a less hydrophobic polymer with increased rates of water absorption, hydrolysis, and erosion. NPs showed no cytotoxicity and may be considered to be appropriate for drug-delivery purposes.

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Section: Chromatographic Conditionsmentioning

confidence: 99%

Cannabinoid derivate-loaded PLGA nanocarriers for oral administration: formulation, characterization, and cytotoxicity studies

Martín-Banderas¹,

Álvarez-Fuentes²,

Durán‐Lobato³

et al. 2012

IJN

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“…The extracted and bound sample is increased and analyzed through a variety of methods, which involve the detection of a chromophore of the bound agent. HPLC analysis is superior to other forms of UV/Vis quantification due to the process ability to clearly quantitate material as well as remove interference problems inherent in other processes [1]. Despite this distinct advantage, HPLC is a very difficult process with variables such as temperature, column choice, mobile phase composition and mobile phase pH all being essential to consider [2].…”

Section: Hplcmentioning

confidence: 99%

“…Dexamethasone: Dexamethasone is a steroid of the glucocorticoid family [1]. These drugs have exhibited effectiveness with treatment of many ocular ailments [11].…”

Section: Clindamycinmentioning

confidence: 99%

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Development and Utilization of Reversed Phase High Performance Liquid Chromatography Methods for a Series of Therapeutic Agents

Corbett¹

2013

Mod Chem Appl

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Capability to quantify drug release is vital in many areas of pharmaceutical research. Accurate, rapid and repeatable detection of pharmaceutical agents allows for comparisons between drug delivery constructs prior to in vivo studies as well as identification of therapeutic levels in samples both in vitro and in vivo. Perhaps the foremost scientific method for monitoring of therapeutics is High Performance Liquid Chromatography. This process allows for clear and simple quantification of therapeutic presence in a sample, with an inherent or induced chromophore, through the detection of bound specimen to the utilized column. Multiple drugs can be used through this process and exemplary methods are presented for the pharmaceutics; cefuroxime, clindamycin, dexamethasone, dicloxacillin, doxycycline, metronidazole, oxymetazoline, paclitaxel, tobramycin, and vancomycin. Each of these drugs is analyzed using Reversed-Phase High Performance Liquid Chromatography utilizing a hydrophobic column. Independent, repeatable methods are developed for each drug. Where necessary a pre-column derivatization is used to allow for visualization of otherwise undetectable tobramycin. In the case of each drug, a standard curve is presented to show the linearity of response for the proposed method within a range of absorbance. This work shows the ability to analyze multiple drugs with a single simple, quick, cost effective system and detection methods are evaluated for each drug with a R 2 value of greater than 0.99.

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“…4 Many methods have been employed to assay dexamethasone in biological materials such as plasma, serum, urine, tears, and hair [5][6][7][8][9] as well as pharmaceutical formulations. [10][11][12][13][14][15][16][17] Besides, methods for the quantification of dexamethasone in association to other drugs have also been reported. 16,[18][19][20] The predominant method in pharmaceutical steroid analyses is the reversed phase HPLC with UV detection, but chromatographic techniques are time consuming, costly and require expertise.…”

Section: Introductionmentioning

confidence: 99%

Validation of a simple and rapid UV spectrophotometric method for dexamethasone assay in tablets

Friedrich¹,

Ravanello²,

Cichota³

et al. 2009

Quím. Nova

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Recebido em 20/5/08; aceito em 29/10/08; publicado na web em 5/2/09This work reports the validation of an analytical UV spectrophotometric method to assay dexamethasone in tablets (assay and dissolution studies). The method was linear in the range between 1 and 30 µg mL -1 presenting a good correlation coefficient (r = 0.9998, n = 7). Precision and accuracy analysis showed low relative standard deviation (< 2.00%) and good percentual recoveries (95-105%). The procedure was linear, accurate, precise, and robust. The method is simple, and it has low cost. It does not use polluting reagents and can be applied in dissolution studies, being an adequate alternative to assay dexamethasone in tablets.

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Determination of dexamethasone acetate in cream by HPLC

Cited by 36 publications

References 5 publications

Cannabinoid derivate-loaded PLGA nanocarriers for oral administration: formulation, characterization, and cytotoxicity studies

Cannabinoid derivate-loaded PLGA nanocarriers for oral administration: formulation, characterization, and cytotoxicity studies

Development and Utilization of Reversed Phase High Performance Liquid Chromatography Methods for a Series of Therapeutic Agents

Validation of a simple and rapid UV spectrophotometric method for dexamethasone assay in tablets

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