Determination of diclofenac in plasma by high-performance liquid chromatography with electrochemical detection

Chmielewska, Agnieszka; Konieczna, Lucyna; Plenis, Alina; Bieniecki, Michał; Lamparczyk, H.

doi:10.1002/bmc.537

Cited by 48 publications

(30 citation statements)

References 22 publications

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“…Literature survey reveals that many analytical methods are reported for determination of DDEA like HPLC [5][6][7] and spectrophotometry.…”

Section: Figurementioning

confidence: 99%

Untitled

2013

IJPSR

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Simple, sensitive and convenient spectrophotometric methods (method A and method B) are developed for the determination of diclofenac diethylamine (DDEA) in pure form and in formulations. Method A is based on the oxidation of drug by Fe(III) followed by the complex formation between reduced form of Fe(III) with 1,10-phenantroline. The resulting red colored chromogen is measured at 510 nm. Method B is based on the formation of charge transfer complex between DDEA (n-donor) and pchloroanilic acid (π-acceptor) in 1,4-dioxane. The resulting violet colored chromogen is measured at 526 nm. Beer's law obeyed in the concentration range of 2.00-8.00 and 20.00-120.00 µg mL -1 for the method A and B, respectively. The molar absorptivity and Sandell's sensitivity are found to be 0.98 × 10 4

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“…Literature survey reveals that many analytical methods are reported for determination of DDEA like HPLC [5][6][7] and spectrophotometry.…”

Section: Figurementioning

confidence: 99%

Untitled

2013

IJPSR

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“…Liquid chromatography using various detection modes has been widely applied. Examples of these reports are HPLC with ultraviolet (UV) detection [4,5], HPLC with electrochemical detection [6], and HPLC with mass spectrometric detection [7]. In addition, other analytical techniques involved the use of potentiometric membrane sensors [8], differential-pulse voltammetry [9], spectrophotometry [10], spectrofluorimetry [11], Raman spectroscopy [12], gas chromatography-mass spectrometry (GC-MS) [13], high-performance thin-layer chromatography (HPTLC) [14], and capillary electrophoresis (CE) [15].…”

Section: Introductionmentioning

confidence: 99%

Validated Selective HPLC-DAD Method for the Simultaneous Determination of Diclofenac Sodium and Lidocaine Hydrochloride in Presence of Four of Their Related Substances and Potential Impurities

Belal¹,

Bedair²,

Gazy³

et al. 2015

Acta Chromatographica

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Summary. This study presents a selective high-performance liquid chromatography (HPLC) with diode array detection (DAD) method for the simultaneous estimation of diclofenac sodium and lidocaine hydrochloride in presence of four of their related substances and potential impurities, namely, 2,6-dimethylaniline (DMA), 2,6-dichloroaniline (DCA), N-phenyl-2,6-dichloroaniline (PDCA), and N-chloroacetyl-N-phenyl-2,6-dichloroaniline (CPDCA). Some of these related substances are reported as degradation products as well. Effective chromatographic separation was achieved using Waters Symmetry C18 column, (3.9 × 150 mm, 5 μm particle size) with gradient elution of the mobile phase composed of 0.05 M orthophosphoric acid and acetonitrile. The gradient elution started with 5% (by volume) acetonitrile, ramped up linearly to 65% in 5 min then kept constant till the end of the run. The mobile phase was pumped at a flow rate of 1.5 mL min −1 . The multiple wavelength detector was set at 220 nm, and quantification of both drugs was based on measuring their peak areas. The retention times for lidocaine and diclofenac were about 5.5 and 9.5 min, respectively. The reliability and analytical performance of the proposed HPLC procedure were statistically validated with respect to system suitability, linearity, ranges, precision, accuracy, specificity, robustness, detection, and quantification limits. Calibration curves were linear in the ranges of 10-200 μg mL −1 for both drugs with correlation coefficients not less than 0.9998. The proposed method proved to be selective by resolution of the two drugs from their related substances and potential impurities. The validated HPLC method was successfully applied to the analysis of this binary mixture in the combined formulation (ampoules dosage form), and the assay results were favorably compared with a previously reported HPLC method. The proposed method made use of DAD as a tool for peak identity and purity confirmation.

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“…They include spectrophotometric [5][6][7][8], fluorometry [9], and chromatography [10][11][12]. However, most of these techniques are time-consuming, laborious to perform or require chemical reagents, thus electrochemical methods have attracted interest, because of the fast response, large sensitivities, simple operation, and the possibilities of miniaturization [12][13][14][15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of Diclofenac Sodium in Aqueous Solution on Cu‐Doped Zeolite‐Expanded Graphite‐Epoxy Electrode

et al. 2010

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The electrochemical oxidation and determination of diclofenac sodium (DCF) at Cu-doped zeolite-modified expanded graphite-epoxy composite (CuZEGE) electrode was evaluated for a new alternative of quantitative determination of sodium diclofenac in aqueous solutions. Cyclic voltammetry was used to characterize the electrochemical behaviour of the electrode in the presence of diclofenac sodium in a 0.1 M NaOH supporting electrolyte. This modified electrode exhibited electrocatalytic effect towards sodium diclofenac oxidation, allowing its determination in aqueous solution. The linear dependence of the current versus diclofenac concentration was obtained using cyclic voltammetry, chronoamperometry, differential-pulsed voltammetry. The limit of detection for DCF reached by direct analysis on CuZEGE is from 2 10 À5 to 3 10 À7 M in relation with used technique and the potential value. Substantial enhancement of the electroanalytical parameters, e.g. the limit of detection of 5 10 À8 M. for the determination of DCF at CuZEGE electrode was reached by applying a chemical preconcentration step prior to voltammetric quantification.

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Determination of diclofenac in plasma by high-performance liquid chromatography with electrochemical detection

Cited by 48 publications

References 22 publications

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Validated Selective HPLC-DAD Method for the Simultaneous Determination of Diclofenac Sodium and Lidocaine Hydrochloride in Presence of Four of Their Related Substances and Potential Impurities

Electrochemical Determination of Diclofenac Sodium in Aqueous Solution on Cu‐Doped Zeolite‐Expanded Graphite‐Epoxy Electrode

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