Determination of diisopropylfluorophosphate in rat plasma and brain tissue by headspace solid‐phase microextraction gas chromatography/mass spectrometry

Xu, Meng; Terry, Alvin V.; Bartlett, Michael G.

doi:10.1002/rcm.3706

Cited by 7 publications

(2 citation statements)

References 27 publications

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“…DFP concentrations in rat plasma and brain tissue were determined by headspace solid-phase microextraction gas chromatography/mass spectrometry as we have described in detail previously (Xu et al, 2008). Briefly, plasma or brain homogenate samples were added to sample vials that had 10 mg of solid sodium fluoride previously added.…”

Section: Methodsmentioning

confidence: 99%

Repeated, intermittent exposures to diisopropylfluorophosphate in rats: protracted effects on cholinergic markers, nerve growth factor-related proteins, and cognitive function

et al. 2011

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Organophosphates (OPs) pose a constant threat to human health due to their widespread use as pesticides and their potential employment in military and terrorist attacks. The acute toxicity of OPs has been extensively studied; however, the consequences of prolonged or repeated exposure to levels of OPs that produce no overt signs of acute toxicity (i.e., subthreshold levels) are poorly understood. Further, there is clinical evidence that such repeated exposures to OPs lead to prolonged deficits in cognition, although the mechanism for this effect is unknown. In this study, the behavioral and neurochemical effects of repeated, intermittent, and subthreshold exposures to the alkyl OP, diisopropylfluorophosphate (DFP) were investigated. Rats were injected with DFP subcutaneously (dose range, 0.25-1.0 mg/kg) every other day over the course of 30 days, and then given a two week, DFP-free washout period. In behavioral experiments conducted at various times during the washout period, dose dependent decrements in a water maze hidden platform task and a spontaneous novel object recognition (NOR) procedure were observed, while prepulse inhibition of the acoustic startle response was unaffected. There were modest decreases in open field locomotor activity and grip strength (particularly during the DFP exposure period); however, rotarod performance and water maze swim speeds were not affected. After washout, DFP concentrations were minimal in plasma and brain, however, cholinesterase inhibition was still © 2010 IBRO. Published by Elsevier Ltd. All rights reserved.Corresponding Author: Alvin V. Terry Jr., Ph.D., Professor of Pharmacology and Toxicology, CB-3618, Medical College of Georgia, 1120 Fifteenth Street, Augusta, Georgia 30912-2450, Phone 706-721-9462, Fax 706-721-2347.edu. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptNeuroscience. Author manuscript; available in PMC 2012 March 10.Published in final edited form as: Neuroscience. 2011 March 10; 176: 237-253. doi:10.1016/j.neuroscience.2010.031. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript detectable in the brain. Moreover, the 1.0 mg/kg dose of DFP was associated with (brain regiondependent) alterations in nerve growth factor-related proteins and cholinergic markers. The results of this prospective animal study thus provide evidence to support two novel hypotheses: 1) that intermittent, subthreshold exposures to alkyl OPs can lead to protracted deficits in specific domains of cognition and 2) that such cognitive deficits may be related to persistent func...

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Section: Methodsmentioning

confidence: 99%

Repeated, intermittent exposures to diisopropylfluorophosphate in rats: protracted effects on cholinergic markers, nerve growth factor-related proteins, and cognitive function

et al. 2011

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“…The advantages provided by SPME made it an important tool for sample preparation. SPME techniques reported in the literature employed different sorbent materials such as polydimethylsiloxane, 4,5 polydimethylsiloxane-divinylbenzene, 6,7 polyacrylate, 8,9 and carboxen-polydimethylsiloxane 10,11 to achieve optimal performance for various compounds. However, the main problem associated with these materials is the lack of selectivity, thus preventing the determination of target analytes at trace levels especially in complex biological samples.…”

Section: Introductionmentioning

confidence: 99%

Microwave-assisted preparation of monolithic molecularly imprinted polymeric fibers for solid phase microextraction

Zhang

Sun

et al. 2013

Analyst

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In this work, a novel microwave-assisted method was developed for fast and batch fabrication of monolithic molecularly imprinted polymer (MIP) fibers. It was based on in situ polymerization in a flexible capillary mold under microwave irradiation. The proposed method greatly shortened the preparation time (about 20-fold) in comparison with the conventional heating. The model template, bisphenol A (BPA) imprinted MIP fiber, was prepared and used for solid phase microextraction (SPME) prior to high performance liquid chromatography (HPLC) determination of BPA and its analogues, i.e., diethylstilbestrol (DES) and hexestrol (HEX). Under the established optimum conditions, the limits of detection for BPA, DES and HEX were 2.0 ng mL(-1), 5.1 ng mL(-1), and 3.3 ng mL(-1), respectively. The fiber-to-fiber extraction (n = 5) was reproducible with the relative standard deviation in the range of 6.6-9.5%. The MIP fiber was used for selective extraction of BPA, DES and HEX in tap water, sewage and urine samples, and the relative recoveries for MIP-SPME-HPLC analysis of BPA, DES and HEX ranged from 82.5% to 103.8%. The advantages provided by this microwave-assisted method will make it a useful tool for preparation of MIP fibers with good SPME performance.

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Current Awareness in Drug Testing and Analysis

2009

Drug Testing and Analysis

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of drug testing and analysis. Each bibliography is divided into 18 sections: 1 Reviews; 2 Sports doping ‐ General; 3 Steroids; 4 Peptides; 5 Diuretics; 6 CNS agents; 7 Equine; 8 Recreational drugs ‐ General; 9 Stimulants; 10 Hallucinogens; 11 Narcotics; 12 Forensics; 13 Alcohol; 14 Tobacco; 15 Homeland security; 16 Workplace; 17 Product authenticity; 18 Techniques. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

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Determination of diisopropylfluorophosphate in rat plasma and brain tissue by headspace solid‐phase microextraction gas chromatography/mass spectrometry

Cited by 7 publications

References 27 publications

Repeated, intermittent exposures to diisopropylfluorophosphate in rats: protracted effects on cholinergic markers, nerve growth factor-related proteins, and cognitive function

Repeated, intermittent exposures to diisopropylfluorophosphate in rats: protracted effects on cholinergic markers, nerve growth factor-related proteins, and cognitive function

Microwave-assisted preparation of monolithic molecularly imprinted polymeric fibers for solid phase microextraction

Current Awareness in Drug Testing and Analysis

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