Determination of dimethyl sulfoxide and dimethyl sulfone in urine by gas chromatography–mass spectrometry after preparation using 2,2‐dimethoxypropane

Abstract

A method for routinely determination of dimethyl sulfoxide (DMSO) and dimethyl sulfone (DMSO(2)) in human urine was developed using gas chromatography-mass spectrometry. The urine sample was treated with 2,2-dimethoxypropane (DMP) and hydrochloric acid for efficient removal of water, which causes degradation of the vacuum level in mass spectrometer and shortens the life-time of the column. Experimental DMP reaction parameters, such as hydrochloric acid concentration, DMP-urine ratio, reaction temperature and reaction time, were optimized for urine. Hexadeuterated DMSO was used as an internal standard. The recoveries of DMSO and DMSO(2) from urine were 97-104 and 98-116%, respectively. The calibration curves showed linearity in the range of 0.15-54.45 mg/L for DMSO and 0.19-50.10 mg/L for DMSO(2). The limits of detection of DMSO and DMSO(2) were 0.04 and 0.06 mg/L, respectively. The relative standard deviations of intra-day and inter-day were 0.2-3.4% for DMSO and 0.4-2.4% for DMSO(2). The proposed method may be useful for the biological monitoring of workers exposed to DMSO in their occupational environment.

Chamber simulation of photooxidation of dimethyl sulfide and isoprene in the presence of NO<sub>x</sub>

“…DMSO 2 in solution using GC-ITMS has been presented by Takeuchi et al (2010). The GC temperature profile in our study is 70 • C for 1 min; ramp to 90 • C at 5 • C min −1 ; ramp to 280 • C at 20 • C min −1 and hold for 8 min.…”

Direct methyl esterification with 2,2-dimethoxypropane for the simultaneous determination of urinary metabolites of toluene, xylene, styrene, and ethylbenzene by gas chromatography-mass spectrometry

“…We adopted a sample preparation using DMP to overcome these disadvantages based on our previous study for the determination of urinary dimethyl sulfoxide and dimethyl sulfone . The reaction scheme for urinary metabolites with DMP is presented in Figure .…”

Methylsulfonylmethane: Applications and Safety of a Novel Dietary Supplement

“…Urine is the most common form of excretion as MSM has been detected in urine of rats [63,67], rabbits [4,5], bobcats [68], cheetahs [69], dogs [70], monkeys [66], and humans [4,62,71,72]. Additionally, excretion of MSM can be contained in feces [63,64] or several other biofluids including cow’s milk [54,73], red deer tail gland secretion [74], and human saliva [75].…”