Determination of Diphenoxylate in Rat Plasma by Gradient Elution Lc-Esi-MS

Wang, Zhiyi; Wang, Zhibin; Chen, Chan; Wang, Jinjin; Chen, Xiaole; Sun, Wei; Ma, Jianshe; Wang, Xianqin

doi:10.1080/10826076.2012.673211

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…The area under curve from zero hour to last hour measurable concentration (AUC 0–t ) and area under curve from zero hour to infinity (AUC 0–α ) for esomeprazole were 8181.486 ng h/mL and 8191.343 ng h/mL, for rabeprazole 1749.192 ng h/mL and 1753.775 ng h/mL and for levosulpiride 1014.206 ng h/mL and 1015.472 ng h/mL. These values were in close proximity when compared with earlier reported values [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] . Fig.…”

Section: Resultssupporting

confidence: 76%

“…As per literature, several liquid chromatography tandem mass spectrometry (LC–MS/MS) methods are reported for the estimation of esomeprazole [15] , [16] , [17] , rabeprazole [18] , [19] , [20] , [21] , [22] and levosulpiride [23] , [24] , [25] individually in biological samples. Till date, there is no ultra pressure liquid chromatography–mass spectrometry (UPLC–MS/MS) method available for the estimation of esomeprazole, rabeprazole and levosulpiride simultaneously in human plasma.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a high throughput UPLC–MS/MS method for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma

Chunduri

Dannana

2016

Journal of Pharmaceutical Analysis

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A high throughput ultra pressure liquid chromatography–mass spectrometry (UPLC–MS/MS) method with good sensitivity and selectivity has been developed and validated for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma using lansoprazole as internal standard (IS). The extraction method based on liquid–liquid extraction technique was used to extract the analytes and IS from of 50 µL of human plasma using methyl tert-butyl ether:ethyl acetate (80:20, v/v), which offers a high recovery. Chromatographic separation of analytes and IS was achieved on a Hypersil gold C18 column using gradient mobile phase consisting of 2 mM ammonium formate/acetonitrile. The flow rate was set at 0.5 mL/min to elute all the analytes and IS within 1.00 min runtime. Detection of target compounds was performed on a triple quadruple mass spectrometer by multiple reaction monitoring (MRM) mode via positive electrospray ionization (ESI). Method validation results demonstrated that the developed method has good precision and accuracy over the concentration ranges of 0.1–2000 ng/mL for each analyte. Stability of compounds was established in a battery of stability studies, i.e., bench top, autosampler, dry extract and long-term storage stability as well as freeze-thaw cycles. The validated method has been successfully applied to analyze human plasma samples for application in pharmacokinetic studies.

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Section: Resultssupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Development and validation of a high throughput UPLC–MS/MS method for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma

Chunduri

Dannana

2016

Journal of Pharmaceutical Analysis

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Chemiluminescence data modeling using parallel factor analysis for the simple and sensitive determination of diphenoxylate in human plasma and tablets

Koohsarian,

Mokhtari,

Hooshmand

2024

Luminescence

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In this study, a chemiluminescence (CL) method was developed to determine diphenoxylate in tablets and human plasma. This is the first CL method proposed to determine diphenoxylate. Creating three‐dimensional data caused the parallel factor analysis algorithm (PARAFAC) to be used for the first time in CL methods. The method is based on the fact that diphenoxylate enhances the weak CL produced in the reaction of Ru(phen)32+ and acidic Ce(IV), and the concentration of Ce(IV) solution has a different effect on the CL response of diphenoxylate and the blank plasma. The calibration curve was linear from 4.0 × 10−8 to 1.6 × 10−6 mol L−1 (R2 = 0.9954), and the detection limit was 1.3 × 10−8 mol L−1 (S/N = 3). The sampling rate was about 30 samples per hour, and the % RSD for 10 repeated measurements of 4 × 10−7 mol L−1 diphenoxylate was 5.4%. The interference effects of some ions, amino acids, and common additives were also investigated. The CL method was successfully used to determine diphenoxylate in tablets, and the results were statistically confirmed by the reference method. The proposed CL method and the PARAFAC algorithm were successfully used to determine the concentration of diphenoxylate in human blood plasma samples.

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A metal organic framework, UiO-66-NH₂, based on a molybdenum Schiff base complex for the efficient electrochemical determination of diphenoxylate

Saeednia,

Rezaeinasab,

Iranmanesh

et al. 2025

Anal. Methods

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Determination of Diphenoxylate in Rat Plasma by Gradient Elution Lc-Esi-MS

Cited by 3 publications

References 13 publications

Development and validation of a high throughput UPLC–MS/MS method for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma

Development and validation of a high throughput UPLC–MS/MS method for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma

Chemiluminescence data modeling using parallel factor analysis for the simple and sensitive determination of diphenoxylate in human plasma and tablets

A metal organic framework, UiO-66-NH₂, based on a molybdenum Schiff base complex for the efficient electrochemical determination of diphenoxylate

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Determination of Diphenoxylate in Rat Plasma by Gradient Elution Lc-Esi-MS

Cited by 3 publications

References 13 publications

Development and validation of a high throughput UPLC–MS/MS method for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma

Development and validation of a high throughput UPLC–MS/MS method for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma

Chemiluminescence data modeling using parallel factor analysis for the simple and sensitive determination of diphenoxylate in human plasma and tablets

A metal organic framework, UiO-66-NH2, based on a molybdenum Schiff base complex for the efficient electrochemical determination of diphenoxylate

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A metal organic framework, UiO-66-NH₂, based on a molybdenum Schiff base complex for the efficient electrochemical determination of diphenoxylate