Determination of dissociation constants of amino acids by capillary zone electrophoresis

Recent advances in amino acid analysis by capillary electrophoresis

“…Strong associations of the solute with the high generations dendrimers were observed [9]. pK a values of underivatized amino acids, deduced from the pH values, as well as the corresponding electrophoretic mobilities were measured using a specially developed program [10]. Indirect UV detection was also used for the analysis of phospho amino acids [11] and amino acids in plants [12] (Fig.…”

Analysis and characterization of phosphinic pseudopeptides by capillary zone electrophoresis

“…Equation (18) was experimentally verified to be valid for uni-univalent aqueous solutions of electrolytes over an ionic strength interval up to 75 mM [23]. Thus, Eq.…”

“…CZE is a suitable method for dissociation constant determination [12][13][14][15][16][17][18] since this technique offers several advantages over commonly used methods such as potentiometric titration and ultraviolet spectroscopy. CZE is capable to overcome drawbacks, such as low solubility in water or purity of compounds, which one can meet by the above-mentioned techniques.…”

Semi-empirical relationships between effective mobility, charge, and molecular weight of pharmaceuticals by pressure-assisted capillary electrophoresis: Applications in drug discovery

“…In the past decade, a number of methods utilizing CE for determination of negative log of the dissociation constant (pK a ) have been reported [1][2][3][4][5][6][7][8][9][10][11]. Since 1998, CE has been employed to determine the pK a s of many different types of molecules including amino acids, pharmaceuticals, metabolites, and radiopharmaceuticals [12][13][14][15][16][17][18][19][20][21][22][23][24][25]. Recently, the major limitation in utilizing CE for rapid determination of pK a , lack of electroosmotic flow (EOF) at low pH, has been overcome using pressure-assisted capillary electrophoresis (PACE) [15,[24][25].…”