Determination of diuretics in human urine using HPLC coupled with magnetic solid‐phase extraction based on a metal–organic framework

Abstract

Magnetic solid‐phase extraction (MSPE) employing a metal–organic framework (Fe3O4@UiO‐66‐OH) combined with high‐performance liquid chromatography was developed for the determination of trace diuretics in urine. The structure and properties of Fe3O4@UiO‐66‐OH were investigated using X‐ray diffraction, infrared spectroscopy, scanning electron microscopy and vibrating sample magnetometry. Magnetic solid‐phase extraction conditions, such as adsorbent amount and solution pH, were optimized using response surface methodology. Under the optimal conditions, the method resulted in excellent linearity with a high correlation coefficient (r > 0.99), satisfactory intraday repeatability (1.78–2.99%), low limits of detection (0.08–0.23 ng/ml), and good recoveries in urine samples (between 93.5 and 103%). Fe3O4@UiO‐66‐OH based on MSPE is a novel pretreatment technique for the detection of trace diuretics in urine.

Metal-Organic Frameworks in Bioanalysis: Extraction of Small Organic Molecules

“…Liu et al [70] synthesized magnetic UiO-66-OH (Fe 3 O 4 @UiO-66-OH) using 2hydroxyterephthalic acid as organic linker and zinc as metal ion. The nanocomposite was employed for the MSPE of diuretics from urine.…”