Determination of Dose Rate of Florfenicol in Feed for Control of Mortality in Channel Catfish <i>Ictalurus punctatus</i> (Rafinesque) Infected with <i>Edwardsiella ictaluri</i>, Etiological Agent of Enteric Septicemia

Gaunt, Patricia S.; Endris, R. G.; Khoo, Lester H.; Howard, R. W.; McGinnis, Anissa L.; Santucci, Timothy D.; Katz, Terry

doi:10.1111/j.1749-7345.2004.tb01083.x

Cited by 30 publications

(29 citation statements)

References 23 publications

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“…In agreement with previously conducted studies with catfish fingerlings, there were no visible gross lesions associated with the FFC treatment (Gaunt et al 2003;Gaunt et al 2004). These comparative mortality data demonstrate that florfenicol administered to catfish infected with F. columnare is safe, significantly reduces mortality, and should be valuable for control of columnaris disease in farmed catfish.…”

Section: Discussionsupporting

confidence: 92%

Efficacy of Florfenicol for Control of Mortality Caused by Flavobacterium columnare Infection in Channel Catfish

Gaunt

Gao

Sun³

et al. 2010

J Aqua Anim Hlth

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The studied in channel catfish Ictalurus punctatus fingerlings held in 80-L aquaria. Nonabraded fish were challenged by immersion on day 0. Thirty 80-L tanks were randomly assigned in equal numbers to two treatment groups, one in which fish were fed a commercial diet without florfenicol (unmedicated feed) and one in which they were fed a diet containing 10 mg of florfenicol/kg of body weight (medicated feed) for ten consecutive days. Mortality was monitored during the treatment period and a 14-d posttreatment observation period. At the end of the posttreatment period, all fish were euthanized, examined for gross lesions, and cultured for F. columnare. Significantly fewer fish fed the medicated diet died (8.0%) than fish fed the unmedicated diet (54.2%). Flavobacterium columnare was cultured from 15.0% of the medicated fish, compared with 68.9% of the unmedicated fish. The gross lesions in the fish were consistent with columnaris disease, and F. columnare was cultured from 99.5% of the dead fish. No differences were observed in weight gain and appetence between the medicated and unmedicated groups. For the F. columnare strain used in this study, the minimal inhibitory concentration of florfenicol ranged from 0.5 to 1.0 mg/mL in the 30 bacterial cultures obtained from infected fish, and the mean disk diffusion zone of inhibition was 40 mm. There were no adverse effects among the medicated fish. Administration of florfenicol at a dosage of 10 mg/kg body weight for 10 d was efficacious and safe for the control of mortality from F. columnare infection in channel catfish.

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Section: Discussionsupporting

confidence: 92%

Efficacy of Florfenicol for Control of Mortality Caused by Flavobacterium columnare Infection in Channel Catfish

Gaunt

Gao

Sun³

et al. 2010

J Aqua Anim Hlth

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“…Therefore, mortality was minimal in the FFC‐medicated fish and ranged only from 0 to 1.25%. In an aquarium dose confirmation study (Gaunt et al 2004), there was a higher percentage of mortalities in FFC‐medicated fish (0.8–2.5%) because treatment was delayed for 2 d after exposure to E. ictaluri . A decrease in appetite was observed, and the fish were overcome by infection and succumbed.…”

Section: Discussionmentioning

confidence: 99%

Field Efficacy of Florfenicol for Control of Mortality in Channel Catfish, Ictalurus punctatus (Rafinesque), Caused by Infection With Edwardsiella ictaluri

Gaunt¹,

McGinnis²,

Santucci³

et al. 2006

J World Aquaculture Soc

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A field study to assess the efficacy of florfenicol (FFC) against enteric septicemia of catfish (ESC) was conducted with pond‐reared channel catfish fingerlings held in 0.1‐acre earthen ponds. Fish were challenged with Edwardsiella ictaluri in a natural pond outbreak or by cohabitation with E. ictaluri‐infected fish held in netpens. Fourteen ponds were assigned in equal number to two treatment groups, that is, either treated (with 10 mg FFC/kg body weight in medicated feed) or not treated (control) for 10 consecutive d. The threshold for enrollment into the study was 0.3% cumulative mortality attributed to ESC. Treatment was initiated on different dates for each pond because each pond was enrolled when 33 fish/pond were diagnosed with ESC based on clinical signs, lesions, or positive cultures. Mortality was monitored during the 10‐d treatment period and during a 14‐d posttreatment observation period. At the end of the 14‐d posttreatment observation period, all fish were euthanized, and 20 fish from each pond were examined by gross necropsy and evaluated for the presence of E. ictaluri by bacterial culture. The odds of a mortality in the control group were 2.20 times the odds of a mortality in the FFC‐treated group. Significantly fewer (P≤ 0.05) FFC‐medicated catfish died in comparison to unmedicated catfish. The minimum inhibitory concentration of FFC for this strain of E. ictaluri was 0.25 μg/mL in all fish that were assayed. The mean zone of inhibition (Kirby Bauer) was 36.8 mm from E. ictaluri isolates of test fish. There were no FFC treatment‐related lesions seen on gross pathology. FFC was efficacious and safe for control of mortality from E. ictaluri infection in catfish.

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“…Fish were hand-fed once per day, and feeding activity was recorded following published methods (Gaunt et al 2004). A numerical score of 2 was assigned to a tank if approximately 50-100% food was consumed.…”

Section: Experimental Designmentioning

confidence: 99%

Efficacy of florfenicol for control of mortality associated with Francisella noatunensis subsp. orientalis in Nile tilapia, Oreochromis niloticus (L.)

Soto

Kidd

Gaunt

et al. 2012

Journal of Fish Diseases

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Francisella noatunensis subsp. orientalis (Fno) (syn. F. asiatica) is an emergent Gram-negative facultative intracellular bacterium. Although it is considered one of the most pathogenic bacteria in fish, there are no commercially available treatments or vaccines. The objective of this project was to determine the most efficacious concentration of florfenicol (FFC) [10, 15 or 20 mg FFC kg(-1) body weight (bw) per days for 10 days] administered in feed to control experimentally induced infections of Fno in Nile tilapia, Oreochromis niloticus (L.), reared in a recirculating aquaculture system. The cumulative mortality of fish that received 0, 10, 15 or 20 mg FFC kg(-1) bw per day was 60, 37, 14 and 16%, respectively. Francisella noatunensis subsp. orientalis genome equivalents were detected in water from all challenged groups with slight reduction in the concentration in the florfenicol-treated groups 4 days after treatment. The mean LOG of CFU Fno mg(-1) spleen was 3-5 and was present in all challenged groups at necropsy 11 days after treatment (21 days after challenge). Results show that florfenicol administered at doses of 15 and 20 mg FFC kg(-1) bw per days for 10 days significantly reduced mortality associated with francisellosis in Nile tilapia.

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Determination of Dose Rate of Florfenicol in Feed for Control of Mortality in Channel Catfish Ictalurus punctatus (Rafinesque) Infected with Edwardsiella ictaluri, Etiological Agent of Enteric Septicemia

Cited by 30 publications

References 23 publications

Efficacy of Florfenicol for Control of Mortality Caused by Flavobacterium columnare Infection in Channel Catfish

Efficacy of Florfenicol for Control of Mortality Caused by Flavobacterium columnare Infection in Channel Catfish

Field Efficacy of Florfenicol for Control of Mortality in Channel Catfish, Ictalurus punctatus (Rafinesque), Caused by Infection With Edwardsiella ictaluri

Efficacy of florfenicol for control of mortality associated with Francisella noatunensis subsp. orientalis in Nile tilapia, Oreochromis niloticus (L.)

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