Determination of effective diffusion coefficients — an important parameters for the efficiency of immobilized biocatalysts

Grünwald, Peter

doi:10.1016/0307-4412(89)90018-6

Cited by 38 publications

(17 citation statements)

References 3 publications

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“…Kinetic parameters for the free PGA and intrinsic kinetic parameters for the immobilized biocatalysts were determined by linearization of initial reaction rate data at Effective diffusion coefficients (D eff ) for penicillin G, PAA and 6-APA were determined through effusion experiments from glyoxyl-agarose particles, by non-linear regression to Equation 1 (Grünwald, 1989):…”

Section: Determination Of Intrinsic Kinetics and Mass Transfer Paramementioning

confidence: 99%

Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading

Illanes¹,

González²,

Gómez³

et al. 2010

Electro Journal of Biotech

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factors at different bulk substrate concentrations were determined for all biocatalysts, values ranging from 0.78 for small particle size at 25 mM penicillin G to 0.15 for large particle size at 2 mM penicillin G. Enzyme protein loading had a strong impact on the effectiveness factors of immobilized penicillin G acylase, being it more pronounced in the case of large particle size biocatalysts. At conditions in which 6-aminopenicillanic acid is industrially produced, all biocatalysts tested were mass-transfer limited, being this information valuable for reactor design and performance evaluation.

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Section: Determination Of Intrinsic Kinetics and Mass Transfer Paramementioning

confidence: 99%

Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading

Illanes¹,

González²,

Gómez³

et al. 2010

Electro Journal of Biotech

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“…The external mass-transfer coefficient, k L , is correlated to external flow velocity and fluid properties by empirical equations (Noordsij and Rotte, 1967). D aq was calculated by an equation (Grunwald, 1989;Zihnioglu and Telefoncu, 1995). The viscosity of the solution was measured by viscometer (TV2000/AKV, Tamson, Holland).…”

Section: Determination Of the Effective Diffusivity Coefficient (D E mentioning

confidence: 99%

Evaluation of effective diffusion coefficient and intrinsic kinetic parameters on azo dye biodegradation using PVA‐immobilized cell beads

Chen

Yang

et al. 2003

Biotech & Bioengineering

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An immobilized mixed culture (Aeromonas hydrophila, Comamonas testosteroni, and Acinetobacter baumannii) was prepared by entrapment into phosphorylated polyvinyl alcohol (PVA) gel beads. The unsteady-state diffusion mechanism in a gel bead was applied to estimate the effective diffusion coefficients (D(e)) and the partition coefficients (K(p)) of azo dye. In addition, a simple method was developed to determine the intrinsic kinetic parameters of immobilized cells from observed reaction rates and the intrinsic kinetic parameters were then verified by fitting the experimental data into the reaction-diffusion model in a batch reactor running at a well-stirred state. The calculated effectiveness factor (eta(cal)) approached unity at Thiele modulus (Phi) < 0.3 (i.e., d(p) < 0.475 mm). The experimental effectiveness factor (eta(exp)) was in the range of 0.71-0.45 for a corresponding sphere diameter (d(p)) of 1.91 +/- 0.16 to 4.43 +/- 0.07 mm at an initial dye concentration of 200 mg/L. The results show that intraparticle diffusion resistance has a significant effect on the azo dye biodegradation rate.

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“…This is therefore in agreement with 2À and Na + ions from activated carbon (650 g/ton of Pt, Pd and Au each) after pre-treatment with a caustic cyanide solution (2 wt% NaCN, 0.6 wt% NaOH) at 80°C. (2) as per Grunwald (1989). b Bas ßtug and Kuyucak (2005).…”

Section: Platinum Mass Balancementioning

confidence: 98%

Determination of the equilibrium and film diffusion constants of the platinum cyanide anions during the elution from activated carbon

Snyders

Bradshaw

Akdogan

et al. 2015

Minerals Engineering

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Determination of effective diffusion coefficients — an important parameters for the efficiency of immobilized biocatalysts

Cited by 38 publications

References 3 publications

Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading

Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading

Evaluation of effective diffusion coefficient and intrinsic kinetic parameters on azo dye biodegradation using PVA‐immobilized cell beads

Determination of the equilibrium and film diffusion constants of the platinum cyanide anions during the elution from activated carbon

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