Determination of eleutheroside E and eleutheroside B in rat plasma and tissue by high-performance liquid chromatography using solid-phase extraction and photodiode array detection

Feng, Shi; Hu, Fang; Zhao, Jun; Liu, Xi; Li, Yun

doi:10.1016/j.ejpb.2005.09.007

Cited by 20 publications

(13 citation statements)

References 3 publications

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“…However, relevance of this in vitro model to the results of clinical studies is supported by pharmacokinetic studies of salidroside, tyrosol, eleutheroside E, and schizandrin. The half-life ( t 1/2 ) was 4.662 h for eleutheroside E (Feng et al, 2006), 4.5 h for salidroside in SHR-5 extract (Panossian et al, 2010), 2 h for tyrosol (Panossian et al, 2009), and 4.6 h for schizandrin (Wang et al, 2008). Pharmacokinetic curve of schizandrin has three maximums of concentration s in blood at 1, 3, and 8 h and it is still detectable in blood at least for 20 h (Wang et al, 2008; Liang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Synergy and Antagonism of Active Constituents of ADAPT-232 on Transcriptional Level of Metabolic Regulation of Isolated Neuroglial Cells

Panossian¹,

Hamm²,

Kadioglu³

et al. 2013

Front. Neurosci.

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Gene expression profiling was performed on the human neuroglial cell line T98G after treatment with adaptogen ADAPT-232 and its constituents – extracts of Eleutherococcus senticosus root, Schisandra chinensis berry, and Rhodiola rosea root as well as several constituents individually, namely, eleutheroside E, schizandrin B, salidroside, triandrin, and tyrosol. A common feature for all tested adaptogens was their effect on G-protein-coupled receptor signaling pathways, i.e., cAMP, phospholipase C (PLC), and phosphatidylinositol signal transduction pathways. Adaptogens may reduce the cAMP level in brain cells by down-regulation of adenylate cyclase gene ADC2Y and up-regulation of phosphodiesterase gene PDE4D that is essential for energy homeostasis as well as for switching from catabolic to anabolic states and vice versa. Down-regulation of cAMP by adaptogens may decrease cAMP-dependent protein kinase A activity in various cells resulting in inhibition stress-induced catabolic transformations and saving of ATP for many ATP-dependant metabolic transformations. All tested adaptogens up-regulated the PLCB1 gene, which encodes phosphoinositide-specific PLC and phosphatidylinositol 3-kinases (PI3Ks), key players for the regulation of NF-κB-mediated defense responses. Other common targets of adaptogens included genes encoding ERα estrogen receptor (2.9–22.6 fold down-regulation), cholesterol ester transfer protein (5.1–10.6 fold down-regulation), heat shock protein Hsp70 (3.0–45.0 fold up-regulation), serpin peptidase inhibitor (neuroserpin), and 5-HT3 receptor of serotonin (2.2–6.6 fold down-regulation). These findings can be reconciled with the observed beneficial effects of adaptogens in behavioral, mental, and aging-associated disorders. Combining two or more active substances in one mixture significantly changes deregulated genes profiles: synergetic interactions result in activation of genes that none of the individual substances affected, while antagonistic interactions result in suppression some genes activated by individual substances. These interactions can have an influence on transcriptional control of metabolic regulation both on the cellular level and the level of the whole organism. Merging of deregulated genes array profiles and intracellular networks is specific to the new substance with unique pharmacological characteristics. Presumably, this phenomenon could be used to eliminate undesirable effects (e.g., toxic effects) and increase the selectivity of pharmacological intervention.

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Section: Discussionmentioning

confidence: 99%

Synergy and Antagonism of Active Constituents of ADAPT-232 on Transcriptional Level of Metabolic Regulation of Isolated Neuroglial Cells

Panossian¹,

Hamm²,

Kadioglu³

et al. 2013

Front. Neurosci.

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“…Based on the obtained results it appeared that the glycoproteins fraction showed higher antimetastatic activity and higher stimulation of the proliferation of splenocytes compared to the water extract [17]. In turn, the performed studies with the use of other cell lines, such [2,19,6]. Other studies showed that the water extract from the stem bark of A. senticosus induced apoptosis in human stomach cancer KATO III cells [4].…”

Section: Discussionmentioning

confidence: 99%

“…The chemical analysis performed by the authors has shown that the investigated species contain eleutherosides B and E, whose content is higher than the requirements of pharmacopoeia. Our earlier studies have shown that eleutherosides B, E and E1 are in all roots and fruits, but not in the leaves [1,2,17].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic activity of ethanolic extracts of Eleutherococcus species cultivated in Poland on HL60 leukemia cell line

Załuski

Smolarz

Bogucka‐Kocka

2014

Current Issues in Pharmacy and Medical Sciences

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The Eleutherococcus species including 40 species native to Asia are medicinal plants widely used in traditional medicine. Some of these species are cultivated at the botanical gardens in Europe. On the basis on our earlier studies it was concluded that the extracts of analyzed species act as antioxidants, inhibitors of MMPs, and cytotoxic against Jurkat 45 leukemia cell line. In this study, the anti-leukemic potential of roots and leaves from six Eleutherococcus species cultivated in Poland was determined. The in vitro cytotoxic activity towards human promyelotic leukemia cell line HL60 using trypan blue assay was evaluated. The induction of apoptosis in stimulated leukemia cells was determined by AnnexinV method. Morphological changes in treated cells were observed by microscopic investigations. The results showed that ethanolic extracts from the roots and the leaves of E. senticosus, E. setchuensis, E. sessiliflorus, E.gracilistylus, E. henryi and E. divaricatus exhibit cytotoxic effect towards leukemic HL60 cells. The received IC50 values for roots ranged from 49-208 µg/mL and for the leaves from 116-518 µg/mL. The ethanol extract from the roots of E. divaricatus showed the highest cytotoxic and proapoptotic effect on HL60 human lymphoid leukemia cell line.

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“…Consequently, it was necessary to set up a method to detect the main indicators of Harpagophytum administration. Apart from recent studies such as those of Seger et al (2005), Baranska et al (2005) and Feng et al (2006), there was very little information in the literature on the analysis and extraction of these compounds from vegetal media and biological fluids. An LC/MS n method was developed at the LCH to detect harpagoside (HS), harpagide (HG) and 8-para-coumaroyl harpagide (8PCHG), which are the major constituents of Harpagophytum procumbens and zeyheri (Colas et al, 2006(Colas et al, , 2008.…”

Section: Introductionmentioning

confidence: 98%

Analysis of iridoids from Harpagophytum and eleutherosides from Eleutherococcus senticosus in horse urine

Colas¹,

Popot²,

Garcia³

et al. 2008

Biomedical Chromatography

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LC/ESI-MS n methods have been previously set up to detect the administration of (i) Harpagophytum and (ii) preparations containing a plant capable of anti-stress properties: Eleutherococcus senticosus. Harpagoside has been found to be the main indicator of Harpagophytum administration in the horse. These methods have been applied to a large number of horse urine samples of various origins. Regarding the detection of Harpagophytum administration, harpagoside, harpagide and 8-para-coumaroyl harpagide were detected together in only one sample out of 317. Eleutheroside E was found to be the main indicator of Eleutherococcus senticosus administration. It was detected in post-administration samples collected from two horses having received a feed supplement containing Eleutherococcus senticosus for several days. Out of the 382 samples tested, eleutheroside E was found in an unexpected large number of urine samples (39%) of various origins and its presence cannot be only due to the sole use of herbal dietary supplements.

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Determination of eleutheroside E and eleutheroside B in rat plasma and tissue by high-performance liquid chromatography using solid-phase extraction and photodiode array detection

Cited by 20 publications

References 3 publications

Synergy and Antagonism of Active Constituents of ADAPT-232 on Transcriptional Level of Metabolic Regulation of Isolated Neuroglial Cells

Synergy and Antagonism of Active Constituents of ADAPT-232 on Transcriptional Level of Metabolic Regulation of Isolated Neuroglial Cells

Cytotoxic activity of ethanolic extracts of Eleutherococcus species cultivated in Poland on HL60 leukemia cell line

Analysis of iridoids from Harpagophytum and eleutherosides from Eleutherococcus senticosus in horse urine

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