Determination of endogenous inflammation-related lipid mediators in ischemic stroke rats using background subtracting calibration curves by liquid chromatography-tandem mass spectrometry

Yang, Yang; Zhong, Qisheng; Mo, Canlong; Zhang, Hao; Zhou, Ting; Tan, Wen

doi:10.1007/s00216-017-0600-7

Cited by 13 publications

(10 citation statements)

References 31 publications

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“…PGs are crucial bioactive mediators contributing to the process of acute inflammation (Stanimirovic & Satoh, 2000). Our previous study presented a significant increase in PGs in ischemic rats, and found that the levels of PGs in rats with STV-Na treatment could be significantly decreased toward normal levels (Yang et al, 2017). In this work, the level of AA in the model group was significantly increased, whereas the AA level was significantly decreased after the intervention of STV-Na.…”

Section: Changes In Aa Metabolismsupporting

confidence: 51%

The investigation of protective effects of isosteviol sodium on cerebral ischemia by metabolomics approach using ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

Yang

Zhang

et al. 2018

Biomedical Chromatography

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Cerebral ischemia remains a major cause of mortality and a long-term disability with limited therapies. Isosteviol sodium (STV-Na) was proved to exert significant protective effects on cerebral ischemia, but the protective mechanism was not understood. In this study, the protective effects of STV-Na on cerebral ischemia were investigated by the metabolomics approach based on ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry technique. The models of ischemic rats were established and the brain tissues were employed for metabolomics analyses. The principal component analysis showed that the model group was clearly separated from the sham group, while both STV-Na and edaravone groups were located between the sham and the model groups, which indicated that STV-Na as well as edaravone had protective effects on cerebral ischemia. Eighteen differential metabolites which had significant differences between the sham and the model groups were screened and identified. After the administration of STV-Na, all 18 differential metabolites were regulated to the levels between the sham and the model groups, and 12 of them presented significant differences between the model and STV-Na groups. The pathway analysis indicated that the protective effects of STV-Na on cerebral ischemia might be associated with the regulation of several metabolic pathways, i.e. glycerophospholipid metabolism, arachidonic acid metabolism and linoleic acid metabolism.

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Section: Changes In Aa Metabolismsupporting

confidence: 51%

The investigation of protective effects of isosteviol sodium on cerebral ischemia by metabolomics approach using ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

Yang

Zhang

et al. 2018

Biomedical Chromatography

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“…Sample storage is also critical due to the high oxidative susceptibility of lipid mediators at room temperature or the enzymatic residual activity. Then, samples are usually stored at -80ºC and AEDT to inhibit PLA 2 activity [174] or antioxidants like BHT are added [124,125,175].…”

Section: Sample Preparation Methodologies For Lipid Mediatorsmentioning

confidence: 99%

“…It provides a fast and proficient extraction with greater selectivity, major capacity for concentrating analytes, less solvent spending and reduced sample handling steps. Both methodologies can be combined to maximize the purification of particularly complex samples and reduce matrix effects in the subsequent LC-MS analysis [105,124,125,[183][184][185][186][187]. SPE stage is also critical for some lipid mediators which are highly unstable like hydroperoxy lipids.…”

Section: Sample Preparation Methodologies For Lipid Mediatorsmentioning

confidence: 99%

“…Other studies suggested the ratio between epoxides/di-hydroxy lipids as a marker of inflammation in CDV diseases [120][121][122]. ARA prostaglandins and leukotrienes have been associated with hypercholesterolemia [48], myocardial reperfusion [50], increased hypertension, obesity [52], heart attacks [123], or ischemic strokes [124]. On the contrary, resolvins and protectins showed beneficial antiinflammatory effectiveness in cardiovascular health [29].…”

Section: Lipid Mediators In Inflammatory Diseases and Metabolic Disordersmentioning

confidence: 99%

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Lipidomic methodologies for biomarkers of chronic inflammation in nutritional research: ω-3 and ω-6 lipid mediators

Dasilva

Medina

2019

Free Radical Biology and Medicine

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The evolutionary history of hominins has been characterized by significant dietary changes, which include the introduction of meat eating, cooking, and the changes associated with plant and animal domestication. The Western pattern diet has been linked with the onset of chronic inflammation, and serious health problems including obesity, metabolic syndrome, and cardiovascular diseases. Diets enriched with ω-3 marine PUFAs have revealed additional improvements in health status associated to a reduction of proinflammatory ω-3 and ω-6 lipid mediators. Lipid mediators are produced from enzymatic and non-enzymatic oxidation of PUFAs. Interest in better understanding the occurrence of these metabolites has increased exponentially as a result of the growing evidence of their role on inflammatory processes, control of the immune system, cell signaling, onset of metabolic diseases, or even cancer. The scope of this review has been to highlight the recent findings on: a) the formation of lipid mediators and their role in different inflammatory and metabolic conditions, b) the direct use of lipid mediators as antiinflammatory drugs or the potential of new drugs as a new therapeutic option for the synthesis of antiinflammatory or resolving lipid mediators and c) the impact of nutritional interventions to modulate lipid mediators synthesis towards antiinflammatory conditions. In a second part, we have summarized methodological approaches (Lipidomics) for the accurate analysis of lipid mediators. Although several techniques have been used, most authors preferred the combination of SPE with LC-MS. Advantages and disadvantages of each method are herein addressed, as well as the main LC-MS difficulties and challenges for the establishment of new biomarkers and standardization of experimental designs, and finally to deepen the study of mechanisms involved on the inflammatory response.

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“…The LLE shows good recoveries for EETs, HETEs and PGs; however, it exhibited lower recoveries for hydrophilic analytes such as tetranor-PGEM and leukotrienes (LTs) [20]. Various SPE materials and protocols are employed and it is inconclusive which material and protocol is the best for extracting oxylipins from biological matrices [24]. …”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of oxylipins in human plasma using reversed-phase liquid chromatography-triple quadrupole mass spectrometry with heatmap-assisted selection of transitions

Chen

Zhang

2018

Anal Bioanal Chem

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Oxylipins, a subclass of lipid mediators, are metabolites of various polyunsaturated fatty acids with crucial functions in regulation of systemic inflammation. Elucidation of their roles in pathological conditions requires accurate quantification of their levels in biological samples. We refined an ultra-performance liquid chromatography-multiple reaction monitoring-mass spectrometry (UPLC-MRM-MS)-based workflow for comprehensive and specific quantification of 131 endogenous oxylipins in human plasma, in which we optimized LC mobile phase additives, column and gradient conditions. We employed heatmap-assisted strategy to identify unique transitions to improve the assay selectivity and optimized solid phase extraction procedures to achieve better analyte recovery. The method was validated according to FDA guidelines. Overall, 94.4% and 95.7% of analytes at tested concentrations were within acceptable accuracy (80–120%) and precision (CV<15%), respectively. Good linearity for most analytes was obtained with R2 > 0.99. The method was also validated using a standard reference material – SRM 1950 frozen human plasma to demonstrate inter-lab compatibility.

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Determination of endogenous inflammation-related lipid mediators in ischemic stroke rats using background subtracting calibration curves by liquid chromatography-tandem mass spectrometry

Cited by 13 publications

References 31 publications

The investigation of protective effects of isosteviol sodium on cerebral ischemia by metabolomics approach using ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

The investigation of protective effects of isosteviol sodium on cerebral ischemia by metabolomics approach using ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry

Lipidomic methodologies for biomarkers of chronic inflammation in nutritional research: ω-3 and ω-6 lipid mediators

Comprehensive analysis of oxylipins in human plasma using reversed-phase liquid chromatography-triple quadrupole mass spectrometry with heatmap-assisted selection of transitions

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