Determination of essential and potentially toxic elements and their estimation of bioaccessibility in honeys

Oliveira, Sönia Sales de; Alves, Camilla Nascimento; Morte, Elane S. Boa; Júnior, Aníbal de Freitas Santos; Araújo, Rennan Geovanny Oliveira; Santos, Daniele Cristina Muniz Batista dos

doi:10.1016/j.microc.2019.104221

Cited by 24 publications

(14 citation statements)

References 34 publications

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“…Flame atomic absorption spectrometry (F-AAS) has been often employed for the low-cost and rapid determination of high concentrations of metals [ 21 , 22 , 28 ]. To control the quality of honey and other beehive products in terms of contamination by heavy metals, many sensitive techniques are required, including graphite furnace atomic absorption spectrophotometry (GF-AAS) [ 17 , 19 ], electrothermal atomic absorption spectrometry (ET-AAS) [ 29 , 30 ], microwave plasma technique atomic emission spectrometry (MP-AES) [ 31 ], inductively coupled plasma optical emission spectrometry (ICP-OES) [ 14 , 16 , 24 , 32 , 33 , 34 ], and inductively coupled plasma mass spectrometry (ICP-MS) [ 14 , 18 , 20 , 35 , 36 , 37 , 38 , 39 ]. In addition, other atomic techniques, such as atomic analyzer mercury (AMA), hydride generation-atomic absorption spectrometry (HG-AAS) and cold vapor atomic fluorescence spectrometry (CV-AFS), have been employed in environmental studies for the determination of Hg, As and Se [ 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

“…Generally, among all beehive products, only honey [ 14 , 20 , 21 , 23 , 32 , 34 , 51 , 52 , 53 ] or honey and pollen [ 17 , 24 , 35 ] are considered in the optimization approach for elemental determination with ICP techniques. To our knowledge, in the literature, few studies are focused on the elemental content of other beehive products such as propolis [ 19 , 31 , 38 ] or royal jelly and beeswax [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness of Different Sample Treatments for the Elemental Characterization of Bees and Beehive Products

et al. 2020

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Bee health and beehive products’ quality are compromised by complex interactions between multiple stressors, among which toxic elements play an important role. The aim of this study is to optimize and validate sensible and reliable analytical methods for biomonitoring studies and the quality control of beehive products. Four digestion procedures, including two systems (microwave oven and water bath) and different mixture reagents, were evaluated for the determination of the total content of 40 elements in bees and five beehive products (beeswax, honey, pollen, propolis and royal jelly) by using inductively coupled plasma mass and optical emission spectrometry. Method validation was performed by measuring a standard reference material and the recoveries for each selected matrix. The water bath-assisted digestion of bees and beehive products is proposed as a fast alternative to microwave-assisted digestion for all elements in biomonitoring studies. The present study highlights the possible drawbacks that may be encountered during the elemental analysis of these biological matrices and aims to be a valuable aid for the analytical chemist. Total elemental concentrations, determined in commercially available beehive products, are presented.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effectiveness of Different Sample Treatments for the Elemental Characterization of Bees and Beehive Products

et al. 2020

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“…To avoid the matrix effect and maximize the signal-to-noise ratio, great attention was paid to the optimization of the sample pre-treatment step [ 44 , 45 , 46 , 47 ]. Although the sample amounts and the instrumental conditions depend on the specific features of the microwave system, according to the recent method proposed by Astolfi et al [ 42 ], HNO 3 and H 2 O 2 were chosen as the acidic/oxidizing mixture due the fact of their proven capability to minimize the formation of the interfering polyatomic ions in the plasma and their efficiency in organic matter decomposition.…”

Section: Resultsmentioning

confidence: 99%

“…From an analytical viewpoint, microwave-assisted digestion [ 41 , 42 ] and inductively coupled plasma spectrometry (ICP-MS) [ 41 , 43 ] are the preferred techniques for sample pre-treatment and for elemental analysis, respectively. Among the former ones, microwave-assisted digestion ensures high efficiency and good performances [ 44 , 45 , 46 , 47 ]. On the other hand, ICP-MS allows to achieve great results in trace and ultra-trace analysis and to perform a reliable investigation for food authentication and traceability [ 13 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Multi-Elemental Analysis as a Tool to Ascertain the Safety and the Origin of Beehive Products: Development, Validation, and Application of an ICP-MS Method on Four Unifloral Honeys Produced in Sardinia, Italy

et al. 2022

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Despite unifloral honeys from Sardinia, Italy, being appreciated worldwide for their peculiar organoleptic features, their elemental signature has only partly been investigated. Hence, the principal aim of this study was to measure the concentration of trace and toxic elements (i.e., Ag, As, Ba, Be, Bi, Cd, Co, Cr, Cu, Fe, Hg, Li, Mn, Mo, Ni, Pb, Sb, Sn, Sr, Te, Tl, V, and Zn) in four unifloral honeys produced in Sardinia. For this purpose, an original ICP-MS method was developed, fully validated, and applied on unifloral honeys from asphodel, eucalyptus, strawberry tree, and thistle. Particular attention was paid to the method’s development: factorial design was applied for the optimization of the acid microwave digestion, whereas the instrumental parameters were tuned to minimize the polyatomic interferences. Most of the analytes’ concentration ranged between the relevant LoDs and few mg kg−1, while toxic elements were present in negligible amounts. The elemental signatures of asphodel and thistle honeys were measured for the first time, whereas those of eucalyptus and strawberry tree honeys suggested a geographical differentiation if compared with the literature. Chemometric analysis allowed for the botanical discrimination of honeys through their elemental signature, whereas linear discriminant analysis provided an accuracy level of 87.1%.

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“…Most of the results showed good data dispersion; however, some standard deviation values were of the same order of magnitude as the intercept data, generating a large statistical uncertainty on these data. The t -test at a 95% confidence level was used to evaluate possible significant differences between the angular coefficients of the calibration curves, in accordance with previous studies [ 25 , 37 , 56 ]. There were no apparent matrix effects between the aqueous and standard addition calibration curves.…”

Section: Resultsmentioning

confidence: 99%

An Analytical Method for the Biomonitoring of Mercury in Bees and Beehive Products by Cold Vapor Atomic Fluorescence Spectrometry

et al. 2021

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Bees and their products are useful bioindicators of anthropogenic activities and could overcome the deficiencies of air quality networks. Among the environmental contaminants, mercury (Hg) is a toxic metal that can accumulate in living organisms. The first aim of this study was to develop a simple analytical method to determine Hg in small mass samples of bees and beehive products by cold vapor atomic fluorescence spectrometry. The proposed method was optimized for about 0.02 g bee, pollen, propolis, and royal jelly, 0.05 g beeswax and honey, or 0.1 g honeydew with 0.5 mL HCl, 0.2 mL HNO3, and 0.1 mL H2O2 in a water bath (95 °C, 30 min); samples were made up to a final volume of 5 mL deionized water. The method limits sample manipulation and the reagent mixture volume used. Detection limits were lower than 3 µg kg−1 for a sample mass of 0.02 g, and recoveries and precision were within 20% of the expected value and less than 10%, respectively, for many matrices. The second aim of the present study was to evaluate the proposed method’s performances on real samples collected in six areas of the Lazio region in Italy.

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Determination of essential and potentially toxic elements and their estimation of bioaccessibility in honeys

Cited by 24 publications

References 34 publications

Effectiveness of Different Sample Treatments for the Elemental Characterization of Bees and Beehive Products

Effectiveness of Different Sample Treatments for the Elemental Characterization of Bees and Beehive Products

Multi-Elemental Analysis as a Tool to Ascertain the Safety and the Origin of Beehive Products: Development, Validation, and Application of an ICP-MS Method on Four Unifloral Honeys Produced in Sardinia, Italy

An Analytical Method for the Biomonitoring of Mercury in Bees and Beehive Products by Cold Vapor Atomic Fluorescence Spectrometry

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